Magnesium in PDB 6n7i: Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
Enzymatic activity of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
All present enzymatic activity of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna):
3.6.4.12;
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
(pdb code 6n7i). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the
Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna), PDB code: 6n7i:
Jump to Magnesium binding site number:
1;
2;
3;
4;
Magnesium binding site 1 out
of 4 in 6n7i
Go back to
Magnesium Binding Sites List in 6n7i
Magnesium binding site 1 out
of 4 in the Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg701
b:37.8
occ:1.00
|
O1B
|
B:TTP700
|
2.2
|
38.3
|
1.0
|
O1G
|
B:TTP700
|
2.2
|
38.3
|
1.0
|
OG
|
B:SER319
|
2.2
|
34.0
|
1.0
|
OE1
|
B:GLN343
|
2.2
|
48.0
|
1.0
|
CD
|
B:GLN343
|
2.9
|
48.0
|
1.0
|
NE2
|
B:GLN343
|
2.9
|
48.0
|
1.0
|
CB
|
B:SER319
|
3.3
|
34.0
|
1.0
|
PB
|
B:TTP700
|
3.4
|
38.3
|
1.0
|
PG
|
B:TTP700
|
3.5
|
38.3
|
1.0
|
O2A
|
B:TTP700
|
3.7
|
38.3
|
1.0
|
O3B
|
B:TTP700
|
3.8
|
38.3
|
1.0
|
O3A
|
B:TTP700
|
4.1
|
38.3
|
1.0
|
N
|
B:SER319
|
4.2
|
34.0
|
1.0
|
CA
|
B:SER319
|
4.3
|
34.0
|
1.0
|
CG
|
B:GLN343
|
4.4
|
48.0
|
1.0
|
O2G
|
B:TTP700
|
4.4
|
38.3
|
1.0
|
O3G
|
B:TTP700
|
4.5
|
38.3
|
1.0
|
PA
|
B:TTP700
|
4.5
|
38.3
|
1.0
|
O2B
|
B:TTP700
|
4.6
|
38.3
|
1.0
|
OD2
|
B:ASP424
|
4.6
|
42.5
|
1.0
|
OD1
|
B:ASP424
|
4.8
|
42.5
|
1.0
|
CB
|
B:GLN343
|
5.0
|
48.0
|
1.0
|
|
Magnesium binding site 2 out
of 4 in 6n7i
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Magnesium Binding Sites List in 6n7i
Magnesium binding site 2 out
of 4 in the Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg701
b:25.9
occ:1.00
|
NE2
|
C:GLN343
|
2.2
|
34.8
|
1.0
|
O1G
|
C:TTP700
|
2.2
|
24.3
|
1.0
|
OG
|
C:SER319
|
2.3
|
23.2
|
1.0
|
OE1
|
C:GLN343
|
2.3
|
34.8
|
1.0
|
O1B
|
C:TTP700
|
2.3
|
24.3
|
1.0
|
CD
|
C:GLN343
|
2.5
|
34.8
|
1.0
|
CB
|
C:SER319
|
3.3
|
23.2
|
1.0
|
PB
|
C:TTP700
|
3.6
|
24.3
|
1.0
|
PG
|
C:TTP700
|
3.6
|
24.3
|
1.0
|
O2A
|
C:TTP700
|
3.9
|
24.3
|
1.0
|
O3B
|
C:TTP700
|
3.9
|
24.3
|
1.0
|
CG
|
C:GLN343
|
4.0
|
34.8
|
1.0
|
OD1
|
C:ASP424
|
4.1
|
31.5
|
1.0
|
O3A
|
C:TTP700
|
4.3
|
24.3
|
1.0
|
CG
|
C:LYS318
|
4.4
|
23.2
|
1.0
|
O2G
|
C:TTP700
|
4.4
|
24.3
|
1.0
|
CA
|
C:SER319
|
4.5
|
23.2
|
1.0
|
N
|
C:SER319
|
4.5
|
23.2
|
1.0
|
OD2
|
C:ASP424
|
4.6
|
31.5
|
1.0
|
O3G
|
C:TTP700
|
4.6
|
24.3
|
1.0
|
PA
|
C:TTP700
|
4.7
|
24.3
|
1.0
|
O2B
|
C:TTP700
|
4.7
|
24.3
|
1.0
|
CG
|
C:ASP424
|
4.7
|
31.5
|
1.0
|
CB
|
C:GLN343
|
4.9
|
34.8
|
1.0
|
CD
|
C:LYS318
|
5.0
|
23.2
|
1.0
|
|
Magnesium binding site 3 out
of 4 in 6n7i
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Magnesium Binding Sites List in 6n7i
Magnesium binding site 3 out
of 4 in the Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg701
b:27.3
occ:1.00
|
O2G
|
D:TTP700
|
2.3
|
24.3
|
1.0
|
O2B
|
D:TTP700
|
2.3
|
24.3
|
1.0
|
OE1
|
D:GLN343
|
2.5
|
37.6
|
1.0
|
OG
|
D:SER319
|
2.8
|
24.8
|
1.0
|
CD
|
D:GLN343
|
3.4
|
37.6
|
1.0
|
CB
|
D:SER319
|
3.4
|
24.8
|
1.0
|
OD1
|
D:ASP424
|
3.6
|
37.7
|
1.0
|
NE2
|
D:GLN343
|
3.6
|
37.6
|
1.0
|
PG
|
D:TTP700
|
3.7
|
24.3
|
1.0
|
PB
|
D:TTP700
|
3.7
|
24.3
|
1.0
|
O3B
|
D:TTP700
|
4.0
|
24.3
|
1.0
|
N
|
D:SER319
|
4.2
|
24.8
|
1.0
|
O2A
|
D:TTP700
|
4.2
|
24.3
|
1.0
|
NZ
|
D:LYS318
|
4.3
|
23.8
|
1.0
|
CA
|
D:SER319
|
4.4
|
24.8
|
1.0
|
CG
|
D:ASP424
|
4.4
|
37.7
|
1.0
|
OD2
|
D:ASP424
|
4.5
|
37.7
|
1.0
|
O3G
|
D:TTP700
|
4.5
|
24.3
|
1.0
|
O1B
|
D:TTP700
|
4.6
|
24.3
|
1.0
|
O1G
|
D:TTP700
|
4.6
|
24.3
|
1.0
|
O3A
|
D:TTP700
|
4.7
|
24.3
|
1.0
|
CG
|
D:GLN343
|
4.8
|
37.6
|
1.0
|
|
Magnesium binding site 4 out
of 4 in 6n7i
Go back to
Magnesium Binding Sites List in 6n7i
Magnesium binding site 4 out
of 4 in the Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna)
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Structure of Bacteriophage T7 E343Q Mutant GP4 Helicase-Primase in Complex with Ssdna, Dttp, Ac Dinucleotide and Ctp (GP4(5)-Dna) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Mg701
b:40.6
occ:1.00
|
O2B
|
E:TTP700
|
2.2
|
37.3
|
1.0
|
OE1
|
E:GLN343
|
2.3
|
49.4
|
1.0
|
O2G
|
E:TTP700
|
2.4
|
37.3
|
1.0
|
OG
|
E:SER319
|
2.6
|
36.1
|
1.0
|
CD
|
E:GLN343
|
3.0
|
49.4
|
1.0
|
NE2
|
E:GLN343
|
3.1
|
49.4
|
1.0
|
PB
|
E:TTP700
|
3.6
|
37.3
|
1.0
|
CB
|
E:SER319
|
3.6
|
36.1
|
1.0
|
PG
|
E:TTP700
|
3.7
|
37.3
|
1.0
|
OD2
|
E:ASP424
|
4.0
|
45.4
|
1.0
|
O3B
|
E:TTP700
|
4.0
|
37.3
|
1.0
|
OD1
|
E:ASP424
|
4.2
|
45.4
|
1.0
|
O3G
|
E:TTP700
|
4.3
|
37.3
|
1.0
|
O2A
|
E:TTP700
|
4.3
|
37.3
|
1.0
|
CG
|
E:GLN343
|
4.4
|
49.4
|
1.0
|
CG
|
E:ASP424
|
4.5
|
45.4
|
1.0
|
O1B
|
E:TTP700
|
4.5
|
37.3
|
1.0
|
N
|
E:SER319
|
4.5
|
36.1
|
1.0
|
O3A
|
E:TTP700
|
4.6
|
37.3
|
1.0
|
NZ
|
E:LYS318
|
4.7
|
35.6
|
1.0
|
O
|
D:HOH801
|
4.7
|
36.7
|
1.0
|
CA
|
E:SER319
|
4.7
|
36.1
|
1.0
|
O1G
|
E:TTP700
|
4.8
|
37.3
|
1.0
|
PA
|
E:TTP700
|
4.9
|
37.3
|
1.0
|
CB
|
E:GLN343
|
5.0
|
49.4
|
1.0
|
O1A
|
E:TTP700
|
5.0
|
37.3
|
1.0
|
|
Reference:
Y.Gao,
Y.Cui,
T.Fox,
S.Lin,
H.Wang,
N.De Val,
Z.H.Zhou,
W.Yang.
Structures and Operating Principles of the Replisome. Science V. 363 2019.
ISSN: ESSN 1095-9203
PubMed: 30679383
DOI: 10.1126/SCIENCE.AAV7003
Page generated: Tue Oct 1 12:34:29 2024
|