Magnesium in PDB 7kls: Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site

Enzymatic activity of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site

All present enzymatic activity of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site:
4.3.3.7;

Protein crystallography data

The structure of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site, PDB code: 7kls was solved by S.Saran, D.A.R.Sanders, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.70 / 2.59
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 85.08, 231.29, 201.54, 90, 90, 90
R / Rfree (%) 21.1 / 25.3

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site (pdb code 7kls). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site, PDB code: 7kls:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in 7kls

Go back to Magnesium Binding Sites List in 7kls
Magnesium binding site 1 out of 3 in the Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg302

b:50.6
occ:1.00
 O A:HOH438 2.2 45.9 1.0
O A:HOH436 2.3 46.7 1.0
OD2 A:ASP177 3.1 50.0 1.0
OD1 A:ASP177 3.2 46.8 1.0
CG A:ASP177 3.5 47.5 1.0
OD1 A:ASP150 4.4 48.1 1.0
CE1 A:HIS181 4.4 44.1 1.0
CG2 A:THR149 4.7 46.5 1.0
CB A:THR149 4.7 46.2 1.0
ND1 A:HIS181 4.8 45.3 1.0

Magnesium binding site 2 out of 3 in 7kls

Go back to Magnesium Binding Sites List in 7kls
Magnesium binding site 2 out of 3 in the Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg302

b:44.1
occ:1.00
 O C:HOH415 2.1 43.2 1.0
OD2 C:ASP227 2.4 42.6 1.0
CG C:ASP227 3.4 43.2 1.0
OD1 C:ASP227 3.9 47.8 1.0
CB C:ASP227 4.7 44.1 1.0

Magnesium binding site 3 out of 3 in 7kls

Go back to Magnesium Binding Sites List in 7kls
Magnesium binding site 3 out of 3 in the Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Dihydrodipicolinate Synthase (Dhdps) From C.Jejuni, H59A Mutant with Pyruvate Bound in the Active Site and L-Lysine Bound at the Allosteric Site within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Mg302

b:48.9
occ:1.00
 OD2 E:ASP227 2.0 44.0 1.0
CG E:ASP227 2.9 41.3 1.0
OD1 E:ASP227 3.0 41.9 1.0
CB E:ASP227 4.3 42.4 1.0

Reference:

S.Saran, Y.Skovpen, M.Majdi Yazdi, D.R.J.Palmer, D.A.R.Sanders. H59 Plays the Most Vital Role in the Transmission of the Allosteric Inhibition Signals in Cj.Dhdps Enzyme To Be Published.
Page generated: Wed Oct 2 22:23:47 2024

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