Magnesium in PDB 7kte: Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN):
2.7.7.7;

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN), PDB code: 7kte was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.96 / 1.48
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.94, 68.523, 110.605, 90, 90, 90
R / Rfree (%)	15.7 / 17.7

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN) also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Sodium	(Na)	1 atom

The binding sites of Magnesium atom in the Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN) (pdb code 7kte). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN), PDB code: 7kte:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:20.1 occ:1.00 OD2 A:ASP330 1.7 34.8 1.0 OP1 P:8OG5 1.8 15.5 0.6 OD2 A:ASP418 2.1 22.2 1.0 OD1 A:ASP332 2.1 22.0 1.0 O A:HOH602 2.1 35.5 1.0 O1A A:8DG506 2.7 19.4 0.4 CG A:ASP330 2.8 36.4 1.0 P P:8OG5 2.8 17.6 0.6 O3' P:DA4 2.9 29.9 0.6 O3' P:DA4 3.0 29.5 0.4 CG A:ASP332 3.1 22.5 1.0 OD1 A:ASP330 3.2 24.0 1.0 CG A:ASP418 3.2 17.1 1.0 OD2 A:ASP332 3.4 21.6 1.0 PA A:8DG506 3.5 20.0 0.4 MG A:MG502 3.5 23.4 1.0 OP2 P:8OG5 3.7 21.8 0.6 O2A A:8DG506 3.8 23.5 0.4 CB A:ASP418 3.9 15.0 1.0 O5' A:8DG506 3.9 22.1 0.4 C3' P:DA4 4.1 23.6 0.6 O5' P:8OG5 4.1 20.5 0.6 CB A:ASP330 4.1 30.2 1.0 C5' P:DA4 4.1 22.8 0.4 C5' P:DA4 4.1 23.1 0.6 C3' P:DA4 4.1 23.6 0.4 CE1 A:HIS329 4.2 26.1 0.5 OD1 A:ASP418 4.2 17.3 1.0 C4' P:DA4 4.2 21.3 0.4 C4' P:DA4 4.2 20.9 0.6 C5' A:8DG506 4.3 20.4 0.4 C5' P:8OG5 4.4 19.3 0.6 CB A:ASP332 4.4 13.9 1.0 NH2 A:ARG416 4.5 16.1 1.0 OP1 P:DA4 4.5 18.9 0.4 O3G A:8DG506 4.7 28.9 0.4 O12 A:PPV505 4.7 24.7 0.2 O A:VAL331 4.8 15.2 1.0 OP1 P:DA4 4.9 19.3 0.6 O5' P:DA4 4.9 20.0 0.6 O5' P:DA4 4.9 19.9 0.4 ND1 A:HIS329 4.9 29.4 0.5 CZ3 A:TRP434 5.0 20.1 1.0 O2B A:8DG506 5.0 25.1 0.4 N A:ASP418 5.0 12.5 1.0

Magnesium binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:23.4 occ:1.00 O1A A:8DG506 1.6 19.4 0.4 O2B A:8DG506 2.0 25.1 0.4 OD1 A:ASP330 2.0 24.0 1.0 O21 A:PPV505 2.1 19.1 0.4 OD2 A:ASP332 2.1 21.6 1.0 O A:HOH733 2.1 26.7 1.0 O12 A:PPV505 2.1 24.7 0.2 O3G A:8DG506 2.1 28.9 0.4 OP1 P:8OG5 2.6 15.5 0.6 PA A:8DG506 3.0 20.0 0.4 PB A:8DG506 3.1 27.0 0.4 CG A:ASP330 3.1 36.4 1.0 P1 A:PPV505 3.2 21.3 0.4 CG A:ASP332 3.2 22.5 1.0 O31 A:PPV505 3.3 20.7 0.4 O3A A:8DG506 3.4 26.6 0.4 PG A:8DG506 3.4 28.2 0.4 P2 A:PPV505 3.4 23.3 0.2 MG A:MG501 3.5 20.1 1.0 OD2 A:ASP330 3.5 34.8 1.0 OD1 A:ASP332 3.6 22.0 1.0 O3B A:8DG506 3.6 28.0 0.4 OPP A:PPV505 3.7 23.5 0.3 P P:8OG5 3.9 17.6 0.6 O A:ASP330 4.0 19.1 1.0 O2A A:8DG506 4.0 23.5 0.4 O1G A:8DG506 4.0 30.4 0.4 O5' A:8DG506 4.0 22.1 0.4 O32 A:PPV505 4.1 26.0 0.2 C5' A:8DG506 4.2 20.4 0.4 ND1 A:HIS329 4.2 29.4 0.5 O5' P:8OG5 4.2 20.5 0.6 C A:ASP330 4.2 21.6 1.0 N A:GLY320 4.3 11.0 1.0 O A:HOH621 4.3 16.1 1.0 C5' P:8OG5 4.3 19.3 0.6 CB A:ASP330 4.4 30.2 1.0 CE1 A:HIS329 4.4 26.1 0.5 O1B A:8DG506 4.5 22.0 0.4 O11 A:PPV505 4.5 18.0 0.4 CA A:GLY319 4.5 12.0 1.0 O A:HOH602 4.5 35.5 1.0 CB A:ASP332 4.5 13.9 1.0 O2G A:8DG506 4.6 31.8 0.4 O22 A:PPV505 4.6 27.4 0.2 OP2 P:8OG5 4.6 21.8 0.6 N A:ASP330 4.7 18.1 1.0 CA A:ASP330 4.7 20.6 1.0 N A:VAL331 4.7 19.8 1.0 O A:HOH770 4.7 29.7 1.0 N A:ASP332 4.8 13.1 1.0 C A:GLY319 5.0 10.9 1.0 C A:VAL331 5.0 14.4 1.0 CA A:VAL331 5.0 15.2 1.0

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:Ct Reaction State Ternary Complex, 50 Mm MG2+ (90MIN) To Be Published.