Magnesium in PDB 7ktf: Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN):
2.7.7.7;

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN), PDB code: 7ktf was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.97 / 1.49
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.947, 68.515, 110.696, 90, 90, 90
R / Rfree (%)	16.7 / 18.7

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN) also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Magnesium atom in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN) (pdb code 7ktf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN), PDB code: 7ktf:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:27.5 occ:1.00 O11 A:PPV504 2.0 18.6 0.6 OD1 A:ASP330 2.1 28.0 1.0 O32 A:PPV504 2.1 28.5 0.6 OD2 A:ASP332 2.1 23.9 1.0 O A:HOH766 2.2 28.9 1.0 OP1 P:8OG5 2.4 25.2 1.0 P1 A:PPV504 3.1 22.4 0.6 CG A:ASP330 3.1 43.1 1.0 CG A:ASP332 3.2 23.4 1.0 O31 A:PPV504 3.3 33.3 0.8 P2 A:PPV504 3.4 22.6 0.4 MG A:MG502 3.5 22.7 1.0 OD2 A:ASP330 3.6 38.9 1.0 OD1 A:ASP332 3.6 23.0 1.0 OPP A:PPV504 3.6 27.2 0.8 P P:8OG5 3.7 27.3 1.0 O12 A:PPV504 4.0 44.2 1.0 O5' P:8OG5 4.0 23.3 1.0 O A:ASP330 4.1 21.5 1.0 O A:HOH831 4.1 45.0 1.0 C5' P:8OG5 4.2 23.0 1.0 N A:GLY320 4.3 12.8 1.0 C A:ASP330 4.3 21.9 1.0 O A:HOH613 4.3 17.5 1.0 O21 A:PPV504 4.4 23.7 0.9 OP2 P:8OG5 4.4 30.0 1.0 CB A:ASP330 4.4 27.6 1.0 CA A:GLY319 4.5 13.6 1.0 CB A:ASP332 4.5 14.9 1.0 O A:HOH605 4.6 31.0 1.0 O22 A:PPV504 4.6 41.1 0.7 CA A:ASP330 4.7 22.4 1.0 N A:ASP330 4.7 20.6 1.0 N A:VAL331 4.8 20.3 1.0 O A:HOH645 4.8 30.8 1.0 N A:ASP332 4.9 13.9 1.0 O3' P:DA4 5.0 27.4 1.0 C A:GLY319 5.0 11.8 1.0

Magnesium binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:22.7 occ:1.00 OD2 A:ASP330 1.7 38.9 1.0 OP1 P:8OG5 1.9 25.2 1.0 OD2 A:ASP418 2.1 21.7 1.0 OD1 A:ASP332 2.1 23.0 1.0 O A:HOH605 2.5 31.0 1.0 CG A:ASP330 2.7 43.1 1.0 P P:8OG5 2.8 27.3 1.0 O3' P:DA4 2.9 27.4 1.0 CG A:ASP332 3.1 23.4 1.0 OD1 A:ASP330 3.2 28.0 1.0 CG A:ASP418 3.2 17.2 1.0 OD2 A:ASP332 3.4 23.9 1.0 MG A:MG501 3.5 27.5 1.0 OP2 P:8OG5 3.7 30.0 1.0 CB A:ASP418 3.9 15.9 1.0 CB A:ASP330 4.1 27.6 1.0 C3' P:DA4 4.1 23.0 1.0 C5' P:DA4 4.1 22.1 1.0 OD1 A:ASP418 4.1 20.1 1.0 O5' P:8OG5 4.1 23.3 1.0 O A:HOH831 4.2 45.0 1.0 C4' P:DA4 4.2 19.0 1.0 C5' P:8OG5 4.4 23.0 1.0 CB A:ASP332 4.5 14.9 1.0 NH2 A:ARG416 4.5 18.3 1.0 O32 A:PPV504 4.7 28.5 0.6 OP1 P:DA4 4.7 20.4 1.0 O A:VAL331 4.7 16.4 1.0 O5' P:DA4 4.9 20.1 1.0 CZ3 A:TRP434 4.9 19.6 1.0 N A:ASP418 4.9 14.0 1.0 C A:VAL331 5.0 15.6 1.0

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (180MIN) To Be Published.