Magnesium in PDB 7ktg: Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN):
2.7.7.7;

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN), PDB code: 7ktg was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.07 / 1.45
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	60.07, 68.523, 110.738, 90, 90, 90
R / Rfree (%)	15.8 / 18.3

The structure of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN) also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Sodium	(Na)	1 atom

The binding sites of Magnesium atom in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN) (pdb code 7ktg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN), PDB code: 7ktg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:17.0 occ:0.76 O12 P:GOA101 2.1 26.2 0.9 OD1 A:ASP330 2.1 28.4 0.6 OD2 A:ASP330 2.1 35.7 0.5 OP1 P:8OG5 2.2 21.7 1.0 OD2 A:ASP332 2.2 25.7 1.0 O A:HOH604 2.3 31.2 1.0 OD1 A:ASP330 2.3 37.0 0.5 CG A:ASP330 2.5 32.6 0.5 C1 P:GOA101 3.1 29.1 0.9 CG A:ASP332 3.3 20.5 1.0 CG A:ASP330 3.3 36.8 0.6 C2 P:GOA101 3.4 37.0 0.9 MG A:MG503 3.4 18.5 1.0 P P:8OG5 3.4 20.4 1.0 OD1 A:ASP332 3.6 22.1 1.0 O5' P:8OG5 3.8 19.8 1.0 OD2 A:ASP330 3.9 29.2 0.6 O A:ASP330 4.0 20.2 0.6 C5' P:8OG5 4.0 22.2 1.0 O A:HOH869 4.0 36.0 1.0 CB A:ASP330 4.1 29.6 0.5 OP2 P:8OG5 4.2 23.8 1.0 O11 P:GOA101 4.3 24.5 0.9 C A:ASP330 4.3 21.6 0.6 N A:GLY320 4.4 12.0 1.0 O P:HOH202 4.5 24.9 1.0 CA A:GLY319 4.5 13.6 1.0 CB A:ASP330 4.5 27.7 0.6 O A:HOH773 4.5 38.1 1.0 O A:ASP330 4.5 19.2 0.5 O A:HOH618 4.5 17.9 1.0 C A:ASP330 4.5 22.0 0.5 O3' P:DA4 4.6 20.7 1.0 CB A:ASP332 4.6 15.0 1.0 CA A:ASP330 4.8 22.0 0.6 CA A:ASP330 4.9 22.1 0.5 N A:VAL331 4.9 20.4 1.0 O A:HOH811 4.9 20.7 0.5 N A:ASP332 5.0 13.4 1.0 N A:ASP330 5.0 19.1 0.6

Magnesium binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg503 b:18.5 occ:1.00 OD2 A:ASP330 1.7 29.2 0.6 OD2 A:ASP330 2.0 35.7 0.5 OD2 A:ASP418 2.1 22.1 1.0 OP1 P:8OG5 2.1 21.7 1.0 OD1 A:ASP332 2.1 22.1 1.0 O P:HOH202 2.4 24.9 1.0 CG A:ASP330 2.5 36.8 0.6 O A:HOH811 2.6 20.7 0.5 OD1 A:ASP330 2.7 28.4 0.6 CG A:ASP418 3.1 17.5 1.0 CG A:ASP332 3.1 20.5 1.0 P P:8OG5 3.1 20.4 1.0 O3' P:DA4 3.1 20.7 1.0 CG A:ASP330 3.2 32.6 0.5 OD2 A:ASP332 3.4 25.7 1.0 MG A:MG502 3.4 17.0 0.8 CB A:ASP418 3.8 15.5 1.0 CB A:ASP330 3.8 29.6 0.5 CB A:ASP330 3.9 27.7 0.6 OP2 P:8OG5 3.9 23.8 1.0 OD1 A:ASP418 4.0 18.9 1.0 C5' P:DA4 4.1 17.0 1.0 OD1 A:ASP330 4.2 37.0 0.5 C3' P:DA4 4.3 18.8 1.0 C4' P:DA4 4.3 16.5 1.0 O A:VAL331 4.4 16.8 1.0 O5' P:8OG5 4.4 19.8 1.0 NH2 A:ARG416 4.4 18.5 1.0 CB A:ASP332 4.5 15.0 1.0 C5' P:8OG5 4.7 22.2 1.0 C A:VAL331 4.7 15.3 1.0 N A:ASP418 4.7 13.7 1.0 OP1 P:DA4 4.8 18.7 1.0 N A:VAL331 4.8 20.4 1.0 CA A:ASP418 4.9 13.6 1.0 CA A:ASP332 4.9 12.8 1.0 CZ3 A:TRP434 4.9 17.8 1.0 C A:ASP330 4.9 21.6 0.6 N A:ASP332 5.0 13.4 1.0

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:Ct Product State Ternary Complex, 50 Mm MG2+ (960MIN) To Be Published.