Magnesium in PDB 7lwa: Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg

The structure of Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg, PDB code: 7lwa was solved by C.L.Tsai, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.52 / 2.85
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.512, 84.722, 95.034, 90, 90, 90
R / Rfree (%)	20.3 / 25.1

The structure of Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg also contains other interesting chemical elements:

Iron

(Fe)

4 atoms

The binding sites of Magnesium atom in the Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg (pdb code 7lwa). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg, PDB code: 7lwa:

Magnesium binding site 1 out of 1 in the Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human Exonuclease 5 Crystal Structure (T88E) in Complex with Ssdna and Mg within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:113.7 occ:1.00 O B:HOH101 2.1 83.5 1.0 O A:HOH501 2.1 79.2 1.0 OE2 A:GLU196 2.1 74.3 1.0 OD2 A:ASP182 2.1 82.1 1.0 OE1 A:GLU196 2.2 60.6 1.0 OD1 A:ASP182 2.2 79.8 1.0 CG A:ASP182 2.3 68.0 1.0 CD A:GLU196 2.5 59.6 1.0 OP1 B:DT2 2.9 109.3 1.0 O3' B:DT1 3.0 118.6 1.0 P B:DT2 3.5 126.3 1.0 CB A:ASP182 3.6 48.0 1.0 CG A:GLU196 4.0 39.1 1.0 C3' B:DT1 4.2 117.9 1.0 OP2 B:DT2 4.3 94.9 1.0 CA A:ASP182 4.5 50.1 1.0 C4' B:DT1 4.5 101.1 1.0 N A:ASP182 4.7 42.1 1.0 NE2 A:GLN217 4.7 50.8 1.0 C2' B:DT1 4.7 128.3 1.0 O A:LEU197 4.7 49.8 1.0 CB A:GLU196 4.8 40.0 1.0 O5' B:DT2 4.9 141.7 1.0 N A:LEU197 4.9 54.2 1.0 CA A:GLU196 4.9 45.1 1.0

S.Hambarde, C.L.Tsai, R.K.Pandita, A.Bacolla, A.Maitra, V.Charaka, C.R.Hunt, R.Kumar, O.Limbo, R.Le Meur, W.J.Chazin, S.E.Tsutakawa, P.Russell, K.Schlacher, T.K.Pandita, J.A.Tainer. EXO5-Dna Structure and Blm Interactions Direct Dna Resection Critical For Atr-Dependent Replication Restart. Mol.Cell 2021.
ISSN: ISSN 1097-2765
PubMed: 34197737
DOI: 10.1016/J.MOLCEL.2021.05.027