Magnesium in PDB 7mbw: Crystal Structure of Tnsc(1-503)A225V

The structure of Crystal Structure of Tnsc(1-503)A225V, PDB code: 7mbw was solved by Y.Shen, A.Guarne, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.50 / 3.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	83.78, 95.77, 313.56, 90, 90, 90
R / Rfree (%)	21.6 / 25.9

The binding sites of Magnesium atom in the Crystal Structure of Tnsc(1-503)A225V (pdb code 7mbw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of Tnsc(1-503)A225V, PDB code: 7mbw:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of Tnsc(1-503)A225V


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Tnsc(1-503)A225V within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:27.7 occ:1.00 OE2 A:GLU233 1.9 52.8 1.0 OG1 A:THR143 2.0 28.6 1.0 O2B A:ADP601 2.1 41.3 1.0 CD A:GLU233 3.0 46.4 1.0 PB A:ADP601 3.1 44.2 1.0 O1B A:ADP601 3.2 32.3 1.0 CB A:THR143 3.4 23.1 1.0 OE1 A:GLU233 3.5 47.9 1.0 O1A A:ADP601 3.9 37.3 1.0 O3B A:ADP601 4.1 20.4 1.0 N A:THR143 4.1 32.8 1.0 OD2 A:ASP232 4.2 42.7 1.0 OD1 A:ASP232 4.2 40.5 1.0 CG2 A:THR143 4.2 30.0 1.0 O3A A:ADP601 4.3 40.5 1.0 CG A:GLU233 4.3 42.9 1.0 CA A:THR143 4.3 22.9 1.0 NZ A:LYS142 4.4 54.3 1.0 PA A:ADP601 4.5 30.9 1.0 O A:HOH705 4.5 27.2 1.0 CG A:ASP232 4.6 48.0 1.0 NE2 A:GLN235 4.7 69.0 1.0 O2A A:ADP601 4.8 31.2 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of Tnsc(1-503)A225V


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Tnsc(1-503)A225V within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg602 b:47.6 occ:1.00 OE2 B:GLU233 2.0 51.2 1.0 O3B B:ADP601 2.1 57.5 1.0 CD B:GLU233 2.8 67.6 1.0 PB B:ADP601 2.9 48.0 1.0 OE1 B:GLU233 3.0 79.0 1.0 O1B B:ADP601 3.0 54.1 1.0 OG1 B:THR143 3.2 40.6 1.0 O2B B:ADP601 3.5 40.2 1.0 NE2 B:GLN235 3.9 82.5 1.0 NZ B:LYS142 4.1 54.9 1.0 CG B:GLU233 4.2 51.3 1.0 CE B:LYS142 4.3 47.4 1.0 O3A B:ADP601 4.3 40.5 1.0 OD1 B:ASP232 4.3 63.6 1.0 CB B:THR143 4.5 36.9 1.0 O1A B:ADP601 4.5 48.7 1.0 N B:THR143 4.8 39.0 1.0 PA B:ADP601 4.9 34.9 1.0 CD B:GLN235 4.9 60.7 1.0 CB B:LYS142 5.0 37.0 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of Tnsc(1-503)A225V


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Tnsc(1-503)A225V within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg602 b:105.8 occ:1.00 O2B C:ADP601 1.8 102.1 1.0 OE2 C:GLU233 1.8 103.0 1.0 PB C:ADP601 2.6 102.9 1.0 CD C:GLU233 2.8 111.8 1.0 O1B C:ADP601 3.1 110.8 1.0 O3B C:ADP601 3.1 114.3 1.0 OG1 C:THR143 3.1 76.6 1.0 OE1 C:GLU233 3.1 110.6 1.0 NE2 C:GLN235 3.4 143.8 1.0 CE C:LYS142 4.0 120.8 1.0 OD1 C:ASP232 4.0 115.3 1.0 O3A C:ADP601 4.1 92.4 1.0 CG C:GLU233 4.1 114.4 1.0 NZ C:LYS142 4.3 126.1 1.0 CB C:THR143 4.3 93.4 1.0 N C:THR143 4.4 93.0 1.0 CB C:LYS142 4.6 104.8 1.0 CD C:GLN235 4.6 145.4 1.0 CG2 C:ILE266 4.6 106.4 1.0 CG C:ASP232 4.9 112.5 1.0 PA C:ADP601 4.9 83.2 1.0 OD2 C:ASP232 4.9 116.9 1.0 CA C:THR143 5.0 93.7 1.0 CA C:GLU233 5.0 126.8 1.0 O2A C:ADP601 5.0 86.0 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of Tnsc(1-503)A225V


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Tnsc(1-503)A225V within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg602 b:78.8 occ:1.00 OE2 D:GLU233 2.0 65.2 1.0 O3B D:ADP601 2.1 65.4 1.0 OG1 D:THR143 2.8 49.0 1.0 PB D:ADP601 3.0 53.1 1.0 O2B D:ADP601 3.1 50.7 1.0 CD D:GLU233 3.1 72.6 1.0 CE D:LYS142 3.6 49.0 1.0 OE1 D:GLU233 3.7 77.9 1.0 OD1 D:ASP232 3.9 78.3 1.0 NZ D:LYS142 3.9 45.2 1.0 N D:THR143 4.0 56.0 1.0 O1B D:ADP601 4.0 50.0 1.0 NE2 D:GLN235 4.0 80.3 1.0 CB D:THR143 4.0 48.1 1.0 CB D:LYS142 4.1 41.2 1.0 O3A D:ADP601 4.3 47.5 1.0 CG D:GLU233 4.3 64.2 1.0 CG2 D:ILE266 4.4 69.2 1.0 CA D:THR143 4.5 56.0 1.0 CD D:LYS142 4.7 61.0 1.0 CG D:ASP232 4.7 82.7 1.0 OD2 D:ASP232 4.7 95.5 1.0 CG D:LYS142 4.8 53.7 1.0 C D:LYS142 4.8 51.7 1.0 CA D:LYS142 4.9 47.4 1.0 OE1 D:GLN235 4.9 83.3 1.0 CD D:GLN235 4.9 68.0 1.0 N D:LYS142 5.0 56.0 1.0 O1A D:ADP601 5.0 45.4 1.0

Y.Shen, J.Gomez-Blanco, M.T.Petassi, J.E.Peters, J.Ortega, A.Guarne. Structural Basis For Dna Targeting By the TN7 Transposon. Nat.Struct.Mol.Biol. V. 29 143 2022.
ISSN: ESSN 1545-9985
PubMed: 35173349
DOI: 10.1038/S41594-022-00724-8