Atomistry » Magnesium » PDB 7mqx-7n46 » 7n2m
Atomistry »
  Magnesium »
    PDB 7mqx-7n46 »
      7n2m »

Magnesium in PDB 7n2m: Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site

Enzymatic activity of Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site

All present enzymatic activity of Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site, PDB code: 7n2m was solved by T.H.Tahirov, A.G.Baranovskiy, N.D.Babayeva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 44.51 / 2.90
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 140.285, 140.285, 181.066, 90, 90, 120
R / Rfree (%) 19.5 / 22.8

Other elements in 7n2m:

The structure of Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site also contains other interesting chemical elements:

Zinc (Zn) 4 atoms
Potassium (K) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site (pdb code 7n2m). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site, PDB code: 7n2m:

Magnesium binding site 1 out of 1 in 7n2m

Go back to Magnesium Binding Sites List in 7n2m
Magnesium binding site 1 out of 1 in the Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Dna Polymerase Alpha Catalytic Core in Complex with Dctp and Template/Primer Having T-C Mismatch at the Post- Insertion Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1302

b:1.8
occ:1.00
OD2 A:ASP860 1.8 43.5 1.0
OD1 A:ASP1004 1.8 20.7 1.0
O A:PHE861 1.9 34.9 1.0
O1B A:DCP1301 1.9 16.4 1.0
O3G A:DCP1301 2.0 26.5 1.0
O1A A:DCP1301 2.5 18.1 1.0
CG A:ASP860 2.7 41.4 1.0
CG A:ASP1004 3.1 22.5 1.0
OD1 A:ASP860 3.1 30.6 1.0
C A:PHE861 3.1 34.2 1.0
PB A:DCP1301 3.3 24.8 1.0
PG A:DCP1301 3.3 24.1 1.0
N A:PHE861 3.7 30.9 1.0
OD2 A:ASP1004 3.7 13.2 1.0
ZN A:ZN1303 3.7 38.4 1.0
PA A:DCP1301 3.8 26.2 1.0
O1G A:DCP1301 3.8 31.1 1.0
O3B A:DCP1301 3.8 22.2 1.0
CA A:PHE861 3.9 32.2 1.0
CB A:ASP860 4.0 35.7 1.0
O3A A:DCP1301 4.1 23.4 1.0
C A:ASP860 4.1 32.7 1.0
N A:ASN862 4.1 32.2 1.0
CB A:ASP1004 4.2 18.4 1.0
C5' A:DCP1301 4.3 18.7 1.0
O2B A:DCP1301 4.3 25.6 1.0
N A:SER863 4.4 22.1 1.0
CB A:PHE861 4.4 28.4 1.0
CA A:ASN862 4.4 28.6 1.0
O2G A:DCP1301 4.5 16.5 1.0
CA A:ASP860 4.6 35.1 1.0
O A:ASP860 4.6 30.4 1.0
O5' A:DCP1301 4.7 12.3 1.0
O A:ASP1004 4.7 29.2 1.0
C A:ASN862 4.7 26.5 1.0
N A:LEU864 4.7 24.7 1.0
O A:HOH1449 4.8 29.9 1.0
O2A A:DCP1301 4.9 21.3 1.0
CB A:LEU864 5.0 23.1 1.0

Reference:

A.G.Baranovskiy, N.D.Babayeva, A.E.Lisova, L.M.Morstadt, T.H.Tahirov. Structural and Functional Insight Into Mismatch Extension By Human Dna Polymerase Alpha. Proc.Natl.Acad.Sci.Usa V. 119 44119 2022.
ISSN: ESSN 1091-6490
PubMed: 35467978
DOI: 10.1073/PNAS.2111744119
Page generated: Thu Oct 3 01:12:55 2024

Last articles

Na in 7Q78
Na in 7Q77
Na in 7Q76
Na in 7Q6Q
Na in 7Q75
Na in 7Q5F
Na in 7Q47
Na in 7Q3Z
Na in 7Q5E
Na in 7Q36
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy