Magnesium in PDB 7o9g: Escherichia Coli Ffh in Complex with Ppgpp

The structure of Escherichia Coli Ffh in Complex with Ppgpp, PDB code: 7o9g was solved by L.Czech, C.-N.Mais, G.Bange, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.26 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	48.63, 38.2, 78.44, 90, 96.27, 90
R / Rfree (%)	19.2 / 26

The binding sites of Magnesium atom in the Escherichia Coli Ffh in Complex with Ppgpp (pdb code 7o9g). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Escherichia Coli Ffh in Complex with Ppgpp, PDB code: 7o9g:

Magnesium binding site 1 out of 1 in the Escherichia Coli Ffh in Complex with Ppgpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Ffh in Complex with Ppgpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:47.6 occ:1.00 OG1 A:THR114 2.6 48.0 1.0 O2B A:G4P401 2.9 52.3 1.0 O1B A:G4P401 3.2 54.8 1.0 OD2 A:ASP138 3.6 59.4 1.0 PB A:G4P401 3.6 49.8 1.0 CB A:THR114 3.9 48.1 1.0 NH2 A:ARG141 4.4 93.2 1.0 O2A A:G4P401 4.4 54.3 1.0 CB A:ALA144 4.5 72.1 1.0 O A:THR191 4.6 55.4 1.0 CG A:ASP138 4.6 60.1 1.0 O3B A:G4P401 4.7 49.3 1.0 CG2 A:THR114 4.7 44.4 1.0 O3A A:G4P401 4.7 49.2 1.0 CG A:LYS113 4.7 52.3 1.0 OD2 A:ASP190 4.7 44.8 1.0 N A:THR114 4.8 48.5 1.0 PA A:G4P401 4.9 45.4 1.0 CA A:THR114 4.9 47.7 1.0

L.Czech, C.N.Mais, H.Kratzat, P.Sarmah, P.Giammarinaro, S.A.Freibert, H.F.Esser, J.Musial, O.Berninghausen, W.Steinchen, R.Beckmann, H.G.Koch, G.Bange. Inhibition of Srp-Dependent Protein Secretion By the Bacterial Alarmone (P)Ppgpp. Nat Commun V. 13 1069 2022.
ISSN: ESSN 2041-1723
PubMed: 35217658
DOI: 10.1038/S41467-022-28675-0