Magnesium in PDB 7r07: Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Protein crystallography data
The structure of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis, PDB code: 7r07
was solved by
M.Figiel,
M.Gapinska,
M.Czarnocki-Cieciura,
W.Zajko,
M.Nowotny,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.63 /
3.10
|
Space group
|
P 62 2 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
200.99,
200.99,
570.72,
90,
90,
120
|
R / Rfree (%)
|
18.9 /
23.2
|
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
(pdb code 7r07). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 9 binding sites of Magnesium where determined in the
Abortive Infection Dna Polymerase Abik From Lactococcus Lactis, PDB code: 7r07:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
Magnesium binding site 1 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 1 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg601
b:129.7
occ:1.00
|
OD2
|
A:ASP247
|
2.3
|
118.3
|
1.0
|
O
|
A:LEU164
|
2.4
|
114.7
|
1.0
|
OD2
|
A:ASP163
|
2.8
|
139.7
|
1.0
|
OD1
|
A:ASP163
|
2.9
|
136.9
|
1.0
|
CG
|
A:ASP163
|
3.2
|
134.1
|
1.0
|
CG
|
A:ASP247
|
3.5
|
113.0
|
1.0
|
C
|
A:LEU164
|
3.6
|
95.7
|
1.0
|
N
|
A:ASN166
|
4.0
|
103.2
|
1.0
|
O3'
|
G:DC10
|
4.2
|
186.3
|
1.0
|
OD1
|
A:ASP247
|
4.2
|
120.7
|
1.0
|
N
|
A:LEU164
|
4.4
|
90.2
|
1.0
|
CA
|
A:SER165
|
4.4
|
91.9
|
1.0
|
N
|
A:SER165
|
4.5
|
91.1
|
1.0
|
N
|
A:PHE167
|
4.5
|
98.0
|
1.0
|
CA
|
A:LEU164
|
4.6
|
84.0
|
1.0
|
C
|
A:SER165
|
4.6
|
98.3
|
1.0
|
CB
|
A:ASP247
|
4.6
|
96.4
|
1.0
|
CB
|
A:ASP163
|
4.7
|
119.7
|
1.0
|
CA
|
A:ASN166
|
4.8
|
108.5
|
1.0
|
NH2
|
A:ARG96
|
4.8
|
150.9
|
1.0
|
C
|
A:ASP163
|
4.9
|
101.2
|
1.0
|
|
Magnesium binding site 2 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 2 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg601
b:182.4
occ:1.00
|
OD2
|
B:ASP247
|
2.5
|
113.5
|
1.0
|
OD1
|
B:ASP163
|
2.7
|
146.8
|
1.0
|
O
|
B:LEU164
|
2.8
|
118.7
|
1.0
|
O3'
|
H:DC10
|
2.9
|
177.9
|
1.0
|
OD2
|
B:ASP163
|
3.4
|
152.7
|
1.0
|
CG
|
B:ASP163
|
3.4
|
143.6
|
1.0
|
CG
|
B:ASP247
|
3.7
|
110.8
|
1.0
|
C
|
B:LEU164
|
4.0
|
107.0
|
1.0
|
OD1
|
B:ASP247
|
4.2
|
115.5
|
1.0
|
C3'
|
H:DC10
|
4.3
|
182.3
|
1.0
|
N
|
B:ASN166
|
4.4
|
100.6
|
1.0
|
N
|
B:LEU164
|
4.6
|
101.3
|
1.0
|
NH2
|
B:ARG96
|
4.7
|
147.4
|
1.0
|
CA
|
B:SER165
|
4.8
|
100.8
|
1.0
|
N
|
B:SER165
|
4.8
|
106.3
|
1.0
|
CA
|
B:LEU164
|
4.9
|
96.0
|
1.0
|
CB
|
B:ASP247
|
4.9
|
98.2
|
1.0
|
CB
|
B:ASP163
|
4.9
|
122.6
|
1.0
|
C4'
|
H:DC10
|
4.9
|
194.4
|
1.0
|
C
|
B:SER165
|
5.0
|
102.0
|
1.0
|
C2'
|
H:DC10
|
5.0
|
185.6
|
1.0
|
|
Magnesium binding site 3 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 3 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg601
b:104.2
occ:1.00
|
OD2
|
C:ASP247
|
2.4
|
99.4
|
1.0
|
O
|
C:LEU164
|
2.5
|
102.0
|
1.0
|
OD1
|
C:ASP163
|
2.7
|
128.0
|
1.0
|
OD2
|
C:ASP163
|
3.0
|
127.1
|
1.0
|
CG
|
C:ASP163
|
3.2
|
122.9
|
1.0
|
MG
|
I:MG101
|
3.3
|
124.2
|
1.0
|
O3'
|
I:DC10
|
3.4
|
219.8
|
1.0
|
CG
|
C:ASP247
|
3.6
|
95.7
|
1.0
|
C
|
C:LEU164
|
3.8
|
98.6
|
1.0
|
OD1
|
C:ASP247
|
4.2
|
102.2
|
1.0
|
N
|
C:ASN166
|
4.3
|
110.7
|
1.0
|
CA
|
C:SER165
|
4.5
|
104.9
|
1.0
|
N
|
C:SER165
|
4.6
|
100.8
|
1.0
|
NH1
|
C:ARG96
|
4.6
|
143.9
|
1.0
|
N
|
C:LEU164
|
4.7
|
87.7
|
1.0
|
CB
|
C:ASP163
|
4.7
|
108.2
|
1.0
|
CA
|
C:LEU164
|
4.8
|
90.8
|
1.0
|
C
|
C:SER165
|
4.8
|
110.4
|
1.0
|
CB
|
C:ASP247
|
4.8
|
82.7
|
1.0
|
C3'
|
I:DC10
|
4.8
|
200.3
|
1.0
|
OD1
|
C:ASP248
|
4.9
|
122.7
|
1.0
|
|
Magnesium binding site 4 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 4 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg601
b:103.9
occ:1.00
|
O
|
D:LEU164
|
2.0
|
163.9
|
1.0
|
OD2
|
D:ASP247
|
2.7
|
98.3
|
1.0
|
OD1
|
D:ASP163
|
2.8
|
144.3
|
1.0
|
MG
|
D:MG602
|
3.2
|
136.9
|
1.0
|
C
|
D:LEU164
|
3.2
|
117.3
|
1.0
|
CG
|
D:ASP163
|
3.5
|
139.4
|
1.0
|
OD2
|
D:ASP163
|
3.5
|
143.8
|
1.0
|
N
|
D:ASN166
|
3.7
|
96.5
|
1.0
|
CG
|
D:ASP247
|
3.9
|
95.2
|
1.0
|
CA
|
D:SER165
|
3.9
|
92.1
|
1.0
|
N
|
D:SER165
|
4.0
|
96.8
|
1.0
|
C
|
D:SER165
|
4.2
|
96.6
|
1.0
|
N
|
D:LEU164
|
4.2
|
109.0
|
1.0
|
CA
|
D:LEU164
|
4.3
|
105.7
|
1.0
|
OD1
|
D:ASP247
|
4.4
|
99.2
|
1.0
|
N
|
D:PHE167
|
4.4
|
106.8
|
1.0
|
O3'
|
J:DC10
|
4.7
|
197.2
|
1.0
|
CA
|
D:ASN166
|
4.7
|
97.4
|
1.0
|
CB
|
D:ASP163
|
4.7
|
126.2
|
1.0
|
CB
|
D:PHE167
|
4.9
|
97.3
|
1.0
|
CB
|
D:LEU164
|
4.9
|
95.0
|
1.0
|
C
|
D:ASP163
|
5.0
|
107.5
|
1.0
|
|
Magnesium binding site 5 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 5 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg602
b:136.9
occ:1.00
|
O3'
|
J:DC10
|
2.2
|
197.2
|
1.0
|
OD2
|
D:ASP163
|
2.8
|
143.8
|
1.0
|
OD1
|
D:ASP247
|
2.9
|
99.2
|
1.0
|
MG
|
D:MG601
|
3.2
|
103.9
|
1.0
|
OD2
|
D:ASP248
|
3.2
|
134.3
|
1.0
|
OD1
|
D:ASP163
|
3.2
|
144.3
|
1.0
|
OD2
|
D:ASP247
|
3.2
|
98.3
|
1.0
|
CG
|
D:ASP163
|
3.4
|
139.4
|
1.0
|
C3'
|
J:DC10
|
3.4
|
192.9
|
1.0
|
CG
|
D:ASP247
|
3.5
|
95.2
|
1.0
|
C4'
|
J:DC10
|
3.9
|
192.8
|
1.0
|
C5'
|
J:DC10
|
4.0
|
194.6
|
1.0
|
CG
|
D:ASP248
|
4.1
|
127.8
|
1.0
|
NZ
|
D:LYS281
|
4.4
|
124.0
|
1.0
|
OD1
|
D:ASP248
|
4.6
|
135.2
|
1.0
|
O
|
D:LEU164
|
4.7
|
163.9
|
1.0
|
C2'
|
J:DC10
|
4.7
|
191.7
|
1.0
|
CB
|
D:ASP163
|
4.8
|
126.2
|
1.0
|
CB
|
D:ASP247
|
5.0
|
86.6
|
1.0
|
|
Magnesium binding site 6 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 6 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Mg601
b:128.6
occ:1.00
|
OP1
|
K:DC11
|
2.3
|
302.3
|
1.0
|
O
|
E:LEU164
|
2.5
|
98.2
|
1.0
|
OD2
|
E:ASP163
|
2.9
|
135.4
|
1.0
|
OD2
|
E:ASP247
|
3.1
|
98.8
|
1.0
|
OD1
|
E:ASP163
|
3.2
|
134.9
|
1.0
|
CG
|
E:ASP163
|
3.4
|
130.2
|
1.0
|
P
|
K:DC11
|
3.6
|
279.7
|
1.0
|
C
|
E:LEU164
|
3.7
|
84.3
|
1.0
|
MG
|
K:MG101
|
3.8
|
130.7
|
1.0
|
N
|
E:ASN166
|
3.8
|
101.6
|
1.0
|
C5'
|
K:DC11
|
3.9
|
252.8
|
1.0
|
O5'
|
K:DC11
|
4.0
|
268.9
|
1.0
|
CA
|
E:SER165
|
4.1
|
77.8
|
1.0
|
CG
|
E:ASP247
|
4.3
|
98.4
|
1.0
|
C
|
E:SER165
|
4.4
|
94.5
|
1.0
|
N
|
E:SER165
|
4.4
|
76.3
|
1.0
|
OP2
|
K:DC11
|
4.6
|
277.3
|
1.0
|
O3'
|
K:DC10
|
4.6
|
264.5
|
1.0
|
N
|
E:LEU164
|
4.7
|
81.0
|
1.0
|
OD1
|
E:ASP247
|
4.7
|
109.0
|
1.0
|
CA
|
E:ASN166
|
4.7
|
103.4
|
1.0
|
N
|
E:PHE167
|
4.8
|
96.5
|
1.0
|
CA
|
E:LEU164
|
4.8
|
77.1
|
1.0
|
CB
|
E:ASP163
|
4.9
|
116.2
|
1.0
|
ND2
|
E:ASN278
|
5.0
|
109.9
|
1.0
|
|
Magnesium binding site 7 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 7 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 7 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Mg601
b:125.7
occ:1.00
|
O
|
F:LEU164
|
2.0
|
164.5
|
1.0
|
OD2
|
F:ASP247
|
2.6
|
111.5
|
1.0
|
OD2
|
F:ASP163
|
2.9
|
139.0
|
1.0
|
OP1
|
L:DC10
|
3.0
|
207.6
|
1.0
|
C
|
F:LEU164
|
3.2
|
126.2
|
1.0
|
C5'
|
L:DC10
|
3.5
|
209.0
|
1.0
|
CG
|
F:ASP163
|
3.5
|
134.3
|
1.0
|
OD1
|
F:ASP163
|
3.6
|
139.8
|
1.0
|
CG
|
F:ASP247
|
3.7
|
103.2
|
1.0
|
O5'
|
L:DC10
|
3.8
|
203.5
|
1.0
|
N
|
F:ASN166
|
3.9
|
99.9
|
1.0
|
P
|
L:DC10
|
4.0
|
199.0
|
1.0
|
N
|
F:SER165
|
4.1
|
108.6
|
1.0
|
CA
|
F:SER165
|
4.1
|
101.0
|
1.0
|
N
|
F:LEU164
|
4.2
|
103.0
|
1.0
|
CA
|
F:LEU164
|
4.2
|
110.2
|
1.0
|
OD1
|
F:ASP247
|
4.3
|
110.7
|
1.0
|
C
|
F:SER165
|
4.4
|
103.3
|
1.0
|
N
|
F:PHE167
|
4.5
|
103.1
|
1.0
|
CB
|
F:LEU164
|
4.7
|
103.6
|
1.0
|
C4'
|
L:DC10
|
4.7
|
207.1
|
1.0
|
CA
|
F:ASN166
|
4.8
|
102.8
|
1.0
|
CB
|
F:ASP163
|
4.8
|
119.7
|
1.0
|
OP2
|
L:DC10
|
4.9
|
194.1
|
1.0
|
O3'
|
L:DC10
|
4.9
|
205.1
|
1.0
|
CB
|
F:ASP247
|
4.9
|
86.0
|
1.0
|
|
Magnesium binding site 8 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 8 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 8 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Mg101
b:124.2
occ:1.00
|
O3'
|
I:DC10
|
2.1
|
219.8
|
1.0
|
C4'
|
I:DC10
|
2.5
|
197.3
|
1.0
|
C3'
|
I:DC10
|
2.8
|
200.3
|
1.0
|
C5'
|
I:DC10
|
2.8
|
189.9
|
1.0
|
OD1
|
C:ASP248
|
3.0
|
122.7
|
1.0
|
OD1
|
C:ASP163
|
3.2
|
128.0
|
1.0
|
MG
|
C:MG601
|
3.3
|
104.2
|
1.0
|
NZ
|
C:LYS281
|
3.7
|
134.2
|
1.0
|
OD1
|
C:ASP247
|
3.9
|
102.2
|
1.0
|
O4'
|
I:DC10
|
3.9
|
192.7
|
1.0
|
OD2
|
C:ASP247
|
3.9
|
99.4
|
1.0
|
C2'
|
I:DC10
|
4.0
|
197.6
|
1.0
|
O5'
|
I:DC10
|
4.0
|
182.8
|
1.0
|
CG
|
C:ASP248
|
4.1
|
113.3
|
1.0
|
CG
|
C:ASP163
|
4.2
|
122.9
|
1.0
|
CG
|
C:ASP247
|
4.3
|
95.7
|
1.0
|
OD2
|
C:ASP248
|
4.4
|
127.9
|
1.0
|
OD2
|
C:ASP163
|
4.6
|
127.1
|
1.0
|
C1'
|
I:DC10
|
4.6
|
192.8
|
1.0
|
NH1
|
C:ARG96
|
4.7
|
143.9
|
1.0
|
OP1
|
I:DC10
|
4.9
|
171.9
|
1.0
|
|
Magnesium binding site 9 out
of 9 in 7r07
Go back to
Magnesium Binding Sites List in 7r07
Magnesium binding site 9 out
of 9 in the Abortive Infection Dna Polymerase Abik From Lactococcus Lactis
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 9 of Abortive Infection Dna Polymerase Abik From Lactococcus Lactis within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
K:Mg101
b:130.7
occ:1.00
|
OP1
|
K:DC11
|
2.0
|
302.3
|
1.0
|
O3'
|
K:DC10
|
2.1
|
264.5
|
1.0
|
P
|
K:DC11
|
2.5
|
279.7
|
1.0
|
OD2
|
E:ASP248
|
2.6
|
131.0
|
1.0
|
OD1
|
E:ASP163
|
3.2
|
134.9
|
1.0
|
C3'
|
K:DC10
|
3.2
|
224.3
|
1.0
|
OD1
|
E:ASP247
|
3.3
|
109.0
|
1.0
|
OP2
|
K:DC11
|
3.3
|
277.3
|
1.0
|
C4'
|
K:DC10
|
3.5
|
202.1
|
1.0
|
C5'
|
K:DC10
|
3.7
|
188.5
|
1.0
|
CG
|
E:ASP248
|
3.7
|
126.5
|
1.0
|
MG
|
E:MG601
|
3.8
|
128.6
|
1.0
|
OD2
|
E:ASP247
|
3.8
|
98.8
|
1.0
|
O5'
|
K:DC11
|
3.9
|
268.9
|
1.0
|
CG
|
E:ASP247
|
4.0
|
98.4
|
1.0
|
CG
|
E:ASP163
|
4.0
|
130.2
|
1.0
|
NZ
|
E:LYS281
|
4.1
|
121.1
|
1.0
|
C5'
|
K:DC11
|
4.2
|
252.8
|
1.0
|
OD1
|
E:ASP248
|
4.3
|
137.3
|
1.0
|
OD2
|
E:ASP163
|
4.3
|
135.4
|
1.0
|
C2'
|
K:DC10
|
4.5
|
211.2
|
1.0
|
O5'
|
K:DC10
|
4.6
|
173.6
|
1.0
|
CE
|
E:LYS281
|
4.7
|
122.8
|
1.0
|
CB
|
E:ASP248
|
4.9
|
107.2
|
1.0
|
O4'
|
K:DC10
|
5.0
|
192.1
|
1.0
|
|
Reference:
M.Figiel,
M.Gapinska,
M.Czarnocki-Cieciura,
W.Zajko,
M.Sroka,
K.Skowronek,
M.Nowotny.
Mechanism of Protein-Primed Template-Independent Dna Synthesis By Abi Polymerases. Nucleic Acids Res. V. 50 10026 2022.
ISSN: ESSN 1362-4962
PubMed: 36107766
DOI: 10.1093/NAR/GKAC772
Page generated: Thu Oct 3 07:04:29 2024
|