Magnesium in PDB 7r0e: Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position

Enzymatic activity of Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position

All present enzymatic activity of Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position:
2.7.7.48;

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position (pdb code 7r0e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position, PDB code: 7r0e:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 7r0e

Go back to Magnesium Binding Sites List in 7r0e
Magnesium binding site 1 out of 2 in the Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg801

b:86.7
occ:1.00
OE2 A:GLU80 2.1 81.4 1.0
OD1 A:ASP108 2.6 82.3 1.0
CD A:GLU80 3.3 81.4 1.0
CG A:ASP108 3.8 82.3 1.0
NE2 A:HIS41 3.8 84.6 1.0
CG A:GLU80 4.1 81.4 1.0
OE1 A:GLU80 4.1 81.4 1.0
OE2 A:GLU119 4.1 87.0 1.0
O A:LEU106 4.1 75.6 1.0
OD2 A:ASP108 4.4 82.3 1.0
O A:PRO107 4.6 78.1 1.0
CD2 A:HIS41 4.6 84.6 1.0
CE1 A:HIS41 4.7 84.6 1.0
C A:PRO107 4.9 78.1 1.0
CB A:ASP108 5.0 82.3 1.0

Magnesium binding site 2 out of 2 in 7r0e

Go back to Magnesium Binding Sites List in 7r0e
Magnesium binding site 2 out of 2 in the Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Early Transcription Elongation State of Influenza A/H7N9 Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 and with Singly Incoporated T1106 at the +1 Position within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg801

b:47.4
occ:1.00
OD1 B:ASP446 1.9 43.2 1.0
O B:HOH930 2.0 44.7 1.0
OD2 B:ASP446 2.3 43.2 1.0
CG B:ASP446 2.3 43.2 1.0
OD1 B:ASP305 2.3 45.1 1.0
O B:HOH925 2.6 43.4 1.0
O B:GLY304 3.2 42.5 1.0
CG B:ASP305 3.5 45.1 1.0
OE1 B:GLU491 3.8 41.6 1.0
CB B:ASP446 3.8 43.2 1.0
OD2 B:ASP305 4.0 45.1 1.0
C B:GLY304 4.1 42.5 1.0
OE2 B:GLU491 4.4 41.6 1.0
O B:PHE447 4.4 41.0 1.0
CD B:GLU491 4.4 41.6 1.0
CA B:ASP446 4.4 43.2 1.0
O B:GLU491 4.5 41.6 1.0
CA B:ASP305 4.5 45.1 1.0
N B:THR493 4.6 41.7 1.0
N B:PHE447 4.6 41.0 1.0
N B:ASP305 4.6 45.1 1.0
CB B:ASP305 4.6 45.1 1.0
CA B:PHE492 4.8 40.1 1.0
N B:GLY304 5.0 42.5 1.0
C B:ASP446 5.0 43.2 1.0

Reference:

T.Kouba, S.Cusack. Direct Observation of Backtracking By Influenza A and B Polymerases Upon Consecutive Incorporation of Nucleoside Analogue T1106 Cell Rep 2022.
ISSN: ESSN 2211-1247
DOI: 10.1016/J.CELREP.2022.111901
Page generated: Thu Oct 3 07:04:30 2024

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