Magnesium in PDB 7r1f: Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106

All present enzymatic activity of Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106:
2.7.7.48;

The binding sites of Magnesium atom in the Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 (pdb code 7r1f). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106, PDB code: 7r1f:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg801 b:39.8 occ:1.00 OD2 B:ASP444 2.3 34.4 1.0 O B:ASN306 2.3 34.7 1.0 OD2 B:ASP305 3.0 37.3 1.0 C B:ASN306 3.5 34.7 1.0 CG B:ASP305 3.5 37.3 1.0 CG B:ASP444 3.5 34.4 1.0 OP1 M:C17 3.6 52.6 1.0 OD1 B:ASP305 3.6 37.3 1.0 OP2 M:U18 4.0 53.8 1.0 MG B:MG802 4.1 40.5 1.0 N B:ASN306 4.2 34.7 1.0 OD1 B:ASP444 4.3 34.4 1.0 N B:THR307 4.3 36.0 1.0 CA B:THR307 4.3 36.0 1.0 C5' M:C17 4.4 52.6 1.0 CA B:ASN306 4.4 34.7 1.0 CB B:TRP309 4.4 35.2 1.0 CB B:ASP444 4.5 34.4 1.0 O5' M:C17 4.7 52.6 1.0 CB B:ASP305 4.7 37.3 1.0 N B:LYS308 4.7 39.1 1.0 N B:TRP309 4.7 35.2 1.0 P M:C17 4.7 52.6 1.0 O5' M:U18 4.8 53.8 1.0 C B:THR307 4.8 36.0 1.0 NZ B:LYS480 4.9 36.7 1.0 P M:U18 4.9 53.8 1.0 O3' M:C17 4.9 52.6 1.0 CB B:ASN306 5.0 34.7 1.0 C B:ASP305 5.0 37.3 1.0

Magnesium binding site 2 out of 3 in the Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg802 b:40.5 occ:1.00 OD1 B:ASP305 2.0 37.3 1.0 OP1 M:C17 2.3 52.6 1.0 CG B:ASP305 2.5 37.3 1.0 OD1 B:ASP444 2.6 34.4 1.0 OD2 B:ASP305 2.7 37.3 1.0 O3' M:U16 3.1 50.1 1.0 OD2 B:ASP444 3.2 34.4 1.0 CG B:ASP444 3.2 34.4 1.0 P M:C17 3.3 52.6 1.0 CB B:ASP445 3.7 35.4 1.0 N B:ASP445 3.8 35.4 1.0 CB B:ASP305 3.8 37.3 1.0 CA B:ASP445 3.9 35.4 1.0 OD2 B:ASP445 4.0 35.4 1.0 CG B:ASP445 4.1 35.4 1.0 MG B:MG801 4.1 39.8 1.0 O5' M:C17 4.3 52.6 1.0 C5' M:C17 4.3 52.6 1.0 OP2 M:C17 4.4 52.6 1.0 C B:ASP444 4.4 34.4 1.0 C3' M:U16 4.4 50.1 1.0 CA B:ASP305 4.6 37.3 1.0 CB B:ASP444 4.6 34.4 1.0 C4' M:U16 4.8 50.1 1.0 O B:ASP444 4.9 34.4 1.0 O2' M:U16 5.0 50.1 1.0

Magnesium binding site 3 out of 3 in the Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Early Transcription Elongation State of Influenza B Polymerase Backtracked Due to Double Incoproation of Nucleotide Analogue T1106 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg803 b:33.9 occ:1.00 OD1 B:ASP445 2.0 35.4 1.0 OD2 B:ASP445 2.5 35.4 1.0 CG B:ASP445 2.5 35.4 1.0 O B:GLY304 3.0 31.1 1.0 OE1 B:GLU490 3.2 34.7 1.0 C B:GLY304 3.6 31.1 1.0 CB B:ASP445 3.9 35.4 1.0 CG2 B:THR303 4.0 31.7 1.0 N B:GLY304 4.1 31.1 1.0 O B:GLU490 4.2 34.7 1.0 O B:PHE446 4.2 31.2 1.0 CD B:GLU490 4.2 34.7 1.0 N B:ASP305 4.3 37.3 1.0 N B:PHE446 4.3 31.2 1.0 CA B:ASP445 4.4 35.4 1.0 CA B:GLY304 4.4 31.1 1.0 CB B:ASP305 4.5 37.3 1.0 OE2 B:GLU490 4.5 34.7 1.0 CA B:ASP305 4.6 37.3 1.0 N B:THR492 4.7 36.8 1.0 CA B:PHE491 4.7 34.3 1.0 C B:THR303 4.7 31.7 1.0 C B:GLU490 4.8 34.7 1.0 C B:ASP445 4.8 35.4 1.0

T.Kouba, S.Cusack. Direct Observation of Backtracking By Influenza A and B Polymerases Upon Consecutive Incorporation of Nucleoside Analogue T1106 Cell Rep 2022.
ISSN: ESSN 2211-1247
DOI: 10.1016/J.CELREP.2022.111901