Magnesium in PDB 7rhw: Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate

All present enzymatic activity of Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate:
4.2.1.11;

The structure of Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate, PDB code: 7rhw was solved by S.Nguyen, J.B.Bruning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.73 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.379, 83.29, 84.161, 90, 97.45, 90
R / Rfree (%)	19 / 22.7

The binding sites of Magnesium atom in the Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate (pdb code 7rhw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate, PDB code: 7rhw:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:31.3 occ:1.00 OE2 A:GLU297 2.0 31.1 1.0 OD2 A:ASP322 2.1 32.6 1.0 O2 A:2PG501 2.1 32.6 1.0 OD2 A:ASP246 2.1 32.1 1.0 O A:HOH645 2.2 29.0 1.0 O1 A:2PG501 2.3 33.9 1.0 C1 A:2PG501 2.6 35.8 1.0 CG A:ASP246 2.9 33.7 1.0 CD A:GLU297 3.1 29.9 1.0 OD1 A:ASP246 3.1 31.4 1.0 CG A:ASP322 3.1 34.1 1.0 CB A:ASP322 3.6 27.7 1.0 NZ A:LYS398 3.6 32.0 1.0 OE1 A:GLU297 3.8 27.8 1.0 O A:HOH601 3.9 40.8 1.0 CG A:GLU297 3.9 26.9 1.0 CD2 A:LEU345 4.0 28.7 1.0 C2 A:2PG501 4.1 36.0 1.0 NZ A:LYS347 4.2 32.5 1.0 OD1 A:ASP322 4.2 32.3 1.0 OD2 A:ASP298 4.2 31.9 1.0 CB A:ASP246 4.3 28.3 1.0 OE1 A:GLN167 4.3 40.1 1.0 OE2 A:GLU168 4.6 33.7 1.0 CE A:LYS347 4.8 30.7 1.0 O1P A:2PG501 4.9 36.1 1.0 C3 A:2PG501 4.9 36.7 1.0 CG A:ASP298 4.9 31.1 1.0 O3 A:2PG501 4.9 36.5 1.0 CE A:LYS398 5.0 30.2 1.0

Magnesium binding site 2 out of 2 in the Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Aspergillus Fumigatus Enolase Bound to 2-Phosphoglycerate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:37.5 occ:1.00 O B:HOH605 2.0 33.1 1.0 OD2 B:ASP322 2.0 32.1 1.0 OE2 B:GLU297 2.1 37.8 1.0 OD2 B:ASP246 2.3 38.2 1.0 O2 B:2PG501 2.3 40.4 0.8 O1 B:2PG501 2.5 36.7 0.8 C1 B:2PG501 2.8 39.1 0.8 CG B:ASP246 3.0 36.6 1.0 OD1 B:ASP246 3.1 33.8 1.0 CG B:ASP322 3.1 35.1 1.0 CD B:GLU297 3.2 32.7 1.0 CB B:ASP322 3.5 27.3 1.0 O B:HOH686 3.8 49.9 1.0 NZ B:LYS398 3.9 30.5 1.0 OD2 B:ASP298 4.0 34.9 1.0 CG B:GLU297 4.0 29.7 1.0 OE1 B:GLU297 4.1 30.9 1.0 OD1 B:ASP322 4.2 31.9 1.0 C2 B:2PG501 4.3 39.8 0.8 CD2 B:LEU345 4.3 32.2 1.0 CB B:ASP246 4.4 33.3 1.0 NZ B:LYS347 4.4 35.0 1.0 O1P B:2PG501 4.7 48.8 0.8 CG B:ASP298 4.7 35.1 1.0 OE1 B:GLN167 4.7 43.0 1.0 OE2 B:GLU168 4.8 45.6 1.0 CA B:ASP322 5.0 29.7 1.0

S.Nguyen, B.Jovcevski, J.Q.Truong, T.L.Pukala, J.B.Bruning. A Structural Model of the Human Plasminogen and Aspergillus Fumigatus Enolase Complex. Proteins V. 90 1509 2022.
ISSN: ESSN 1097-0134
PubMed: 35247004
DOI: 10.1002/PROT.26331