Magnesium in PDB 7sq2: Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex

All present enzymatic activity of Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex:
3.1.4.11;

The structure of Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex, PDB code: 7sq2 was solved by S.T.Endo-Streeter, J.Sondek, T.K.Harden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.55 / 2.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	202.837, 90.817, 93.135, 90, 101.19, 90
R / Rfree (%)	19.9 / 26.3

The structure of Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex also contains other interesting chemical elements:

Aluminium	(Al)	1 atom
Calcium	(Ca)	1 atom
Fluorine	(F)	4 atoms

The binding sites of Magnesium atom in the Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex (pdb code 7sq2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex, PDB code: 7sq2:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:59.1 occ:1.00 O2B A:GDP403 2.3 44.4 1.0 OG1 A:THR186 2.4 65.5 1.0 OG A:SER53 2.5 57.8 1.0 O A:HOH503 2.7 54.8 1.0 O1B A:GDP403 2.8 35.2 1.0 CB A:THR186 2.9 69.1 1.0 PB A:GDP403 3.0 65.8 1.0 F3 A:ALF401 3.1 75.6 1.0 F1 A:ALF401 3.1 103.3 1.0 AL A:ALF401 3.4 67.5 1.0 O A:VAL206 3.5 68.2 1.0 OD1 A:ASP205 3.5 91.7 1.0 CG2 A:THR186 3.6 70.3 1.0 CB A:SER53 3.7 60.8 1.0 NZ A:LYS52 3.7 77.8 1.0 N A:SER53 4.0 67.2 1.0 CE A:LYS52 4.0 73.2 1.0 O3B A:GDP403 4.0 70.8 1.0 CA A:THR186 4.2 64.2 1.0 OD2 A:ASP205 4.2 83.2 1.0 CG A:ASP205 4.2 81.1 1.0 N A:THR186 4.3 67.8 1.0 CB A:LYS52 4.3 66.9 1.0 F2 A:ALF401 4.3 129.7 1.0 O3A A:GDP403 4.4 45.4 1.0 CA A:SER53 4.4 68.9 1.0 O1A A:GDP403 4.4 41.7 1.0 F4 A:ALF401 4.5 85.8 1.0 C A:VAL206 4.6 71.8 1.0 PA A:GDP403 4.8 58.3 1.0 O2A A:GDP403 4.8 83.2 1.0 C A:LYS52 4.9 69.6 1.0 O A:THR186 5.0 66.8 1.0 CA A:LYS52 5.0 66.6 1.0

Magnesium binding site 2 out of 2 in the Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Reprocessed and Refined Structure of Phospholipase C-Beta and Gq Signaling Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg908 b:90.4 occ:1.00 O B:HOH1056 2.7 74.1 1.0 O B:GLU304 3.6 76.0 1.0 O B:GLN254 3.9 72.9 1.0 CB B:GLN254 4.4 75.4 1.0 NH1 B:ARG817 4.5 57.1 1.0 CG2 B:ILE250 4.6 76.5 1.0 OH B:TYR299 4.6 84.4 1.0 C B:GLN254 4.6 76.5 1.0 C B:GLU304 4.7 77.3 1.0 CZ B:ARG817 4.7 61.4 1.0 CE1 B:HIS821 4.7 79.2 1.0 O B:HOH1080 4.8 79.2 1.0 OD1 B:ASN305 4.8 67.7 1.0 CA B:ASN305 4.9 67.0 1.0 NE B:ARG817 5.0 66.2 1.0

S.T.Endo-Streeter, J.Sondek, T.K.Harden. Kinetic Scaffolding Mediated By A Phospholipase C-{Beta} and Gq Signaling Complex Science V. 330 974 2010.
ISSN: ISSN 0036-8075
PubMed: 20966218
DOI: 10.1126/SCIENCE.1193438