Magnesium in PDB 7tgn: Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway

The structure of Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway, PDB code: 7tgn was solved by K.D.Patel, A.M.Gulick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.47 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	108.53, 108.53, 517.75, 90, 90, 90
R / Rfree (%)	19.6 / 23

The binding sites of Magnesium atom in the Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway (pdb code 7tgn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway, PDB code: 7tgn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg605 b:61.8 occ:1.00 O B:HOH727 2.5 51.4 1.0 O B:HOH716 2.6 53.6 1.0 OD1 A:ASN242 2.8 50.8 1.0 OE2 A:GLU166 2.9 57.1 1.0 OD1 B:ASN242 2.9 51.0 1.0 OE2 B:GLU166 3.1 54.5 1.0 OE1 A:GLU166 3.4 53.9 1.0 CG A:ASN242 3.5 51.0 1.0 CD A:GLU166 3.5 55.1 1.0 CG B:ASN242 3.6 53.6 1.0 ND2 A:ASN242 3.7 50.2 1.0 CD B:GLU166 3.8 52.2 1.0 OE1 B:GLU166 3.8 53.3 1.0 ND2 B:ASN242 3.9 52.5 1.0 NE2 B:HIS165 4.6 51.5 1.0 CB A:ASN242 4.7 48.1 1.0 NE1 A:TRP238 4.7 50.6 1.0 CE1 B:HIS165 4.8 52.9 1.0 NE2 A:HIS165 4.8 56.1 1.0 CE1 A:HIS165 4.8 54.2 1.0 CB B:ASN242 4.9 50.5 1.0 CG A:GLU166 4.9 50.8 1.0 NE1 B:TRP238 5.0 51.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Desd, the Desferrioxamine Synthetase From the Streptomyces Violaceus Salmycin Biosynthetic Pathway within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg605 b:54.2 occ:1.00 O C:HOH737 2.4 49.7 1.0 O D:HOH757 2.5 49.8 1.0 OD1 D:ASN242 2.8 49.4 1.0 OE2 C:GLU166 3.0 54.2 1.0 ND2 C:ASN242 3.1 45.5 1.0 OE2 D:GLU166 3.2 52.7 1.0 CG D:ASN242 3.5 51.0 1.0 OE1 C:GLU166 3.5 52.6 1.0 CD C:GLU166 3.6 54.6 1.0 OE1 D:GLU166 3.7 50.9 1.0 CG C:ASN242 3.7 49.8 1.0 ND2 D:ASN242 3.8 48.4 1.0 CD D:GLU166 3.8 53.7 1.0 OD1 C:ASN242 3.9 50.1 1.0 CB D:ASN242 4.7 47.6 1.0 NE2 C:HIS165 4.8 54.0 1.0 CE1 C:HIS165 4.8 55.1 1.0 O2 D:EDO604 4.8 63.3 1.0 NE1 C:TRP238 4.8 49.8 1.0 NE1 D:TRP238 4.9 48.6 1.0 CB C:ASN242 4.9 45.2 1.0 C2 C:EDO602 5.0 62.5 1.0

J.Yang, V.S.Banas, K.D.Patel, G.S.M.Rivera, L.S.Mydy, A.M.Gulick, T.A.Wencewicz. An Acyl-Adenylate Mimic Reveals the Structural Basis For Substrate Recognition By the Iterative Siderophore Synthetase Desd. J.Biol.Chem. V. 298 02166 2022.
ISSN: ESSN 1083-351X
PubMed: 35750210
DOI: 10.1016/J.JBC.2022.102166