Magnesium in PDB 7uea: Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)

The structure of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) also contains other interesting chemical elements:

Iron	(Fe)	12 atoms
Calcium	(Ca)	2 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 30; Page 4, Binding sites: 31 - 40; Page 5, Binding sites: 41 - 50; Page 6, Binding sites: 51 - 54;

Binding sites:

The binding sites of Magnesium atom in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) (pdb code 7uea). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 54 binding sites of Magnesium where determined in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1), PDB code: 7uea:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg801 b:73.4 occ:1.00 MG A:GS0801 0.0 73.4 1.0 ND A:GS0801 1.9 61.9 1.0 NB A:GS0801 1.9 62.2 1.0 NA A:GS0801 2.2 62.4 1.0 NC A:GS0801 2.3 41.9 1.0 NE2 A:HIS621 2.7 52.1 1.0 C4D A:GS0801 2.8 62.5 1.0 C1B A:GS0801 2.8 57.4 1.0 C4B A:GS0801 3.0 53.2 1.0 C1D A:GS0801 3.1 58.3 1.0 C4A A:GS0801 3.1 63.9 1.0 C1A A:GS0801 3.2 53.8 1.0 OBB a:GS0804 3.2 64.6 1.0 C1C A:GS0801 3.2 48.7 1.0 CHB A:GS0801 3.2 60.1 1.0 CHA A:GS0801 3.3 52.6 1.0 C4C A:GS0801 3.3 52.7 1.0 CHC A:GS0801 3.4 55.8 1.0 CHD A:GS0801 3.5 60.4 1.0 CE1 A:HIS621 3.6 52.8 1.0 CD2 A:HIS621 3.7 49.3 1.0 C2B A:GS0801 4.0 54.4 1.0 C3D A:GS0801 4.0 59.1 1.0 CAB a:GS0804 4.1 61.3 1.0 C3B A:GS0801 4.1 52.5 1.0 C2D A:GS0801 4.2 60.5 1.0 C3B a:GS0804 4.2 54.5 1.0 C3A A:GS0801 4.4 65.8 1.0 C2A A:GS0801 4.5 57.0 1.0 C2C A:GS0801 4.5 54.0 1.0 C2B a:GS0804 4.6 53.3 1.0 C3C A:GS0801 4.6 57.6 1.0 C4B a:GS0804 4.6 49.8 1.0 ND1 A:HIS621 4.7 51.9 1.0 CBD A:GS0801 4.7 49.2 1.0 CG A:HIS621 4.8 54.4 1.0 CD1 A:LEU688 4.9 60.6 1.0 CHC a:GS0804 5.0 47.8 1.0

Magnesium binding site 2 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg802 b:80.0 occ:1.00 MG A:BCL802 0.0 80.0 1.0 ND A:BCL802 2.0 65.4 1.0 NB A:BCL802 2.1 71.7 1.0 NC A:BCL802 2.1 71.7 1.0 NA A:BCL802 2.1 67.0 1.0 NE2 A:HIS79 2.9 65.8 1.0 C4D A:BCL802 3.0 66.8 1.0 C4B A:BCL802 3.1 71.3 1.0 C1C A:BCL802 3.1 74.7 1.0 C1B A:BCL802 3.1 68.6 1.0 C1D A:BCL802 3.1 65.0 1.0 C4C A:BCL802 3.1 68.1 1.0 C4A A:BCL802 3.1 63.5 1.0 C1A A:BCL802 3.2 65.1 1.0 CHC A:BCL802 3.4 75.0 1.0 CHB A:BCL802 3.4 70.1 1.0 CHD A:BCL802 3.4 69.6 1.0 CHA A:BCL802 3.4 66.8 1.0 CMB A:BCL811 3.6 69.5 1.0 CE1 A:HIS79 3.7 67.3 1.0 CD2 A:HIS79 3.9 61.5 1.0 C3D A:BCL802 4.2 74.5 1.0 C2D A:BCL802 4.3 70.5 1.0 C2B A:BCL802 4.3 68.4 1.0 C3B A:BCL802 4.3 67.8 1.0 CE1 A:PHE279 4.3 57.3 1.0 C2C A:BCL802 4.3 76.8 1.0 C3A A:BCL802 4.4 64.7 1.0 C3C A:BCL802 4.4 70.6 1.0 C2A A:BCL802 4.5 64.9 1.0 C9 A:F26815 4.6 79.8 1.0 C15 A:F26815 4.7 78.8 1.0 CD1 A:PHE279 4.7 55.4 1.0 CBD A:BCL802 4.9 69.5 1.0 ND1 A:HIS79 4.9 71.5 1.0 CMA A:BCL802 5.0 70.9 1.0 CG A:HIS79 5.0 62.4 1.0

Magnesium binding site 3 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg803 b:64.0 occ:1.00 MG A:BCL803 0.0 64.0 1.0 ND A:BCL803 2.0 65.3 1.0 NB A:BCL803 2.1 69.4 1.0 NC A:BCL803 2.1 61.8 1.0 NA A:BCL803 2.2 69.7 1.0 C4D A:BCL803 3.0 67.8 1.0 C4B A:BCL803 3.1 74.0 1.0 C1B A:BCL803 3.1 70.5 1.0 C1C A:BCL803 3.1 64.9 1.0 C1D A:BCL803 3.1 67.3 1.0 C4C A:BCL803 3.1 68.5 1.0 C4A A:BCL803 3.1 70.7 1.0 C1A A:BCL803 3.2 68.4 1.0 CHC A:BCL803 3.4 72.7 1.0 CHB A:BCL803 3.4 74.6 1.0 CHA A:BCL803 3.4 68.2 1.0 CHD A:BCL803 3.4 72.8 1.0 C52 A:F39814 3.9 69.8 1.0 C3D A:BCL803 4.2 69.7 1.0 C2B A:BCL803 4.2 67.8 1.0 C3B A:BCL803 4.3 71.9 1.0 C2D A:BCL803 4.3 68.8 1.0 C2C A:BCL803 4.4 68.0 1.0 C2A A:BCL803 4.4 69.4 1.0 C3C A:BCL803 4.4 69.0 1.0 C3A A:BCL803 4.4 67.6 1.0 CZ3 A:TRP411 4.4 75.1 1.0 CE3 A:TRP411 4.6 75.1 1.0 CBD A:BCL803 4.9 68.8 1.0 CAA A:BCL803 4.9 69.3 1.0

Magnesium binding site 4 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg804 b:77.1 occ:1.00 MG A:BCL804 0.0 77.1 1.0 ND A:BCL804 2.0 67.2 1.0 NB A:BCL804 2.1 72.9 1.0 NC A:BCL804 2.1 70.2 1.0 NA A:BCL804 2.2 63.6 1.0 NE2 A:HIS150 2.8 75.9 1.0 C4D A:BCL804 3.0 69.9 1.0 C4B A:BCL804 3.0 67.2 1.0 C1B A:BCL804 3.1 69.1 1.0 C14 A:BCL808 3.1 77.5 1.0 C1C A:BCL804 3.1 68.0 1.0 C1D A:BCL804 3.1 71.3 1.0 C4C A:BCL804 3.1 72.2 1.0 C4A A:BCL804 3.1 69.1 1.0 C1A A:BCL804 3.2 64.9 1.0 CHC A:BCL804 3.4 71.8 1.0 CD2 A:HIS150 3.4 75.1 1.0 CHB A:BCL804 3.4 70.4 1.0 CHA A:BCL804 3.4 71.0 1.0 CHD A:BCL804 3.4 76.6 1.0 CE1 A:HIS150 3.5 79.9 1.0 C3D A:BCL804 4.2 69.6 1.0 C2B A:BCL804 4.2 65.0 1.0 C3B A:BCL804 4.2 64.0 1.0 C2D A:BCL804 4.2 69.8 1.0 CG A:HIS150 4.3 69.3 1.0 ND1 A:HIS150 4.4 77.3 1.0 C2C A:BCL804 4.4 66.0 1.0 C3C A:BCL804 4.4 69.2 1.0 C13 A:BCL808 4.4 76.1 1.0 C3A A:BCL804 4.4 72.9 1.0 C2A A:BCL804 4.5 69.1 1.0 CBD A:BCL804 4.9 76.9 1.0 C15 A:BCL808 4.9 72.1 1.0 CBC A:BCL804 5.0 73.9 1.0

Magnesium binding site 5 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg805 b:83.2 occ:1.00 MG A:BCL805 0.0 83.2 1.0 ND A:BCL805 2.0 70.4 1.0 NB A:BCL805 2.0 77.8 1.0 NC A:BCL805 2.1 74.8 1.0 NA A:BCL805 2.2 71.7 1.0 C4D A:BCL805 2.9 66.0 1.0 CE1 A:HIS164 3.0 71.9 1.0 NE2 A:HIS164 3.0 70.9 1.0 C4B A:BCL805 3.0 79.4 1.0 C1B A:BCL805 3.1 75.0 1.0 C1C A:BCL805 3.1 72.7 1.0 C1D A:BCL805 3.1 69.8 1.0 C4C A:BCL805 3.1 78.7 1.0 C4A A:BCL805 3.1 73.7 1.0 C1A A:BCL805 3.2 71.0 1.0 CHC A:BCL805 3.4 75.4 1.0 CHB A:BCL805 3.4 75.5 1.0 CHA A:BCL805 3.4 70.4 1.0 CHD A:BCL805 3.4 78.2 1.0 C7 A:BCL805 4.1 81.6 1.0 C3D A:BCL805 4.2 70.1 1.0 C2D A:BCL805 4.2 68.8 1.0 C2B A:BCL805 4.3 71.7 1.0 C3B A:BCL805 4.3 78.7 1.0 ND1 A:HIS164 4.3 73.4 1.0 C2C A:BCL805 4.3 73.9 1.0 C3A A:BCL805 4.4 80.1 1.0 CD2 A:HIS164 4.4 68.5 1.0 C3C A:BCL805 4.4 77.1 1.0 C2A A:BCL805 4.4 80.9 1.0 C6 A:BCL805 4.5 79.7 1.0 CD2 A:LEU169 4.5 75.3 1.0 C5 A:BCL805 4.7 74.3 1.0 CBD A:BCL805 4.8 79.0 1.0

Magnesium binding site 6 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg806 b:79.2 occ:1.00 MG A:BCL806 0.0 79.2 1.0 ND A:BCL806 2.0 70.5 1.0 NB A:BCL806 2.1 66.7 1.0 NC A:BCL806 2.1 69.9 1.0 NA A:BCL806 2.2 70.7 1.0 NE2 A:HIS209 2.6 63.1 1.0 C4D A:BCL806 3.0 68.5 1.0 C4B A:BCL806 3.1 62.7 1.0 C1B A:BCL806 3.1 75.4 1.0 C1C A:BCL806 3.1 68.3 1.0 C1D A:BCL806 3.1 72.1 1.0 C4C A:BCL806 3.1 76.2 1.0 C4A A:BCL806 3.1 69.9 1.0 C1A A:BCL806 3.2 67.6 1.0 OBB A:BCL805 3.2 88.3 1.0 CHC A:BCL806 3.4 67.8 1.0 CHB A:BCL806 3.4 77.4 1.0 CD2 A:HIS209 3.4 63.8 1.0 CHA A:BCL806 3.4 69.7 1.0 CHD A:BCL806 3.4 76.4 1.0 CE1 A:HIS209 3.5 66.3 1.0 CAB A:BCL805 4.2 82.2 1.0 C3D A:BCL806 4.2 70.9 1.0 C2D A:BCL806 4.3 71.0 1.0 C2B A:BCL806 4.3 78.3 1.0 C3B A:BCL806 4.3 71.4 1.0 C3C A:BCL806 4.4 73.8 1.0 C2C A:BCL806 4.4 73.5 1.0 C3A A:BCL806 4.4 67.5 1.0 C2A A:BCL806 4.5 71.2 1.0 CG A:HIS209 4.5 57.8 1.0 ND1 A:HIS209 4.5 66.9 1.0 O2D A:BCL806 4.7 80.5 1.0 CBB A:BCL805 4.8 77.8 1.0 CMB A:BCL805 4.8 71.5 1.0 CBD A:BCL806 4.8 76.2 1.0

Magnesium binding site 7 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg807 b:76.5 occ:1.00 MG A:BCL807 0.0 76.5 1.0 ND A:BCL807 2.0 70.1 1.0 NB A:BCL807 2.1 73.2 1.0 NC A:BCL807 2.1 64.1 1.0 NA A:BCL807 2.2 70.7 1.0 C4D A:BCL807 3.0 61.5 1.0 C4B A:BCL807 3.1 71.8 1.0 C1B A:BCL807 3.1 69.8 1.0 C1C A:BCL807 3.1 65.0 1.0 C1D A:BCL807 3.1 69.8 1.0 C4C A:BCL807 3.1 64.8 1.0 C4A A:BCL807 3.1 71.8 1.0 C1A A:BCL807 3.2 65.7 1.0 CHC A:BCL807 3.4 69.8 1.0 CHB A:BCL807 3.4 70.9 1.0 CHA A:BCL807 3.4 58.3 1.0 CHD A:BCL807 3.4 72.0 1.0 CD A:GLU242 3.5 81.2 1.0 OE1 A:GLU242 3.5 83.7 1.0 C24 A:F39814 3.5 66.2 1.0 OE2 A:GLU242 3.7 84.3 1.0 NH1 A:ARG120 3.9 72.8 1.0 CG A:GLU242 4.0 74.2 1.0 C23 A:F39814 4.1 70.4 1.0 C3D A:BCL807 4.2 63.8 1.0 C2B A:BCL807 4.2 68.3 1.0 C2D A:BCL807 4.3 68.9 1.0 C3B A:BCL807 4.3 69.2 1.0 C2C A:BCL807 4.4 64.9 1.0 C3C A:BCL807 4.4 60.0 1.0 C3A A:BCL807 4.4 71.2 1.0 C2A A:BCL807 4.5 72.3 1.0 CB A:GLU242 4.6 69.9 1.0 CZ A:PHE306 4.7 66.3 1.0 C22 A:F39814 4.8 69.0 1.0 CBD A:BCL807 4.9 65.5 1.0 CZ A:ARG120 4.9 76.8 1.0 CE2 A:PHE306 4.9 67.7 1.0 C26 A:F39814 5.0 71.2 1.0

Magnesium binding site 8 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg808 b:71.2 occ:1.00 MG A:BCL808 0.0 71.2 1.0 ND A:BCL808 2.0 72.3 1.0 NB A:BCL808 2.1 72.0 1.0 NC A:BCL808 2.1 72.5 1.0 NA A:BCL808 2.2 60.8 1.0 C4D A:BCL808 3.0 76.3 1.0 NE2 A:HIS282 3.0 70.0 1.0 C4B A:BCL808 3.1 73.6 1.0 C1B A:BCL808 3.1 73.7 1.0 C1C A:BCL808 3.1 72.2 1.0 C1D A:BCL808 3.1 67.0 1.0 C4C A:BCL808 3.1 68.6 1.0 C4A A:BCL808 3.1 71.4 1.0 C1A A:BCL808 3.2 66.3 1.0 CHC A:BCL808 3.4 76.9 1.0 CHB A:BCL808 3.4 76.2 1.0 CHA A:BCL808 3.4 70.1 1.0 CHD A:BCL808 3.4 68.8 1.0 CD2 A:HIS282 3.6 64.4 1.0 CE1 A:HIS282 3.6 72.8 1.0 C33 A:LMG822 4.1 90.0 1.0 C34 A:LMG822 4.2 90.9 1.0 C3D A:BCL808 4.2 75.7 1.0 C2B A:BCL808 4.2 74.3 1.0 C2D A:BCL808 4.3 69.6 1.0 C3B A:BCL808 4.3 71.9 1.0 ND1 A:HIS282 4.4 69.9 1.0 CG A:HIS282 4.4 59.5 1.0 C2C A:BCL808 4.4 70.2 1.0 C2A A:BCL808 4.4 73.4 1.0 C3C A:BCL808 4.4 69.8 1.0 C3A A:BCL808 4.4 76.5 1.0 C31 A:LMG822 4.8 89.0 1.0 CBD A:BCL808 4.9 66.4 1.0

Magnesium binding site 9 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg809 b:51.1 occ:1.00 MG A:BCL809 0.0 51.1 1.0 ND A:BCL809 2.0 59.2 1.0 NB A:BCL809 2.1 64.1 1.0 NC A:BCL809 2.1 51.5 1.0 NA A:BCL809 2.2 48.4 1.0 ND2 A:ASN375 2.9 64.5 1.0 C4D A:BCL809 3.0 54.6 1.0 C4B A:BCL809 3.1 61.3 1.0 C1B A:BCL809 3.1 64.2 1.0 C1D A:BCL809 3.1 65.5 1.0 C1C A:BCL809 3.1 59.3 1.0 C4C A:BCL809 3.1 57.1 1.0 C4A A:BCL809 3.1 59.0 1.0 C1A A:BCL809 3.2 52.0 1.0 CG A:ASN375 3.2 60.9 1.0 OD1 A:ASN375 3.3 74.2 1.0 CHC A:BCL809 3.4 62.8 1.0 CHB A:BCL809 3.4 63.7 1.0 CHD A:BCL809 3.4 67.3 1.0 CHA A:BCL809 3.4 52.5 1.0 C2 A:BCL809 4.1 58.9 1.0 C3D A:BCL809 4.2 59.0 1.0 O2A A:BCL809 4.2 76.7 1.0 CB A:ASN375 4.2 57.1 1.0 C2D A:BCL809 4.2 62.5 1.0 C2B A:BCL809 4.3 61.8 1.0 C3B A:BCL809 4.3 58.5 1.0 C2C A:BCL809 4.3 64.2 1.0 C3C A:BCL809 4.4 61.4 1.0 C3A A:BCL809 4.4 62.5 1.0 CMB A:BCL810 4.5 63.7 1.0 C2A A:BCL809 4.5 59.1 1.0 C1 A:BCL809 4.6 66.8 1.0 CA A:ASN375 4.8 55.5 1.0 CGA A:BCL809 4.8 72.6 1.0 C20 A:F26815 4.9 74.0 1.0 CBD A:BCL809 4.9 57.9 1.0

Magnesium binding site 10 out of 54 in the Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Photosynthetic Assembly of Chlorobaculum Tepidum (Rc-FMO1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg810 b:70.7 occ:1.00 MG A:BCL810 0.0 70.7 1.0 ND A:BCL810 2.0 63.7 1.0 NB A:BCL810 2.1 72.9 1.0 NC A:BCL810 2.1 64.7 1.0 NA A:BCL810 2.2 67.7 1.0 NE2 A:HIS376 2.8 54.5 1.0 C4D A:BCL810 2.9 62.4 1.0 C4B A:BCL810 3.0 68.8 1.0 C1B A:BCL810 3.1 64.4 1.0 C1C A:BCL810 3.1 65.1 1.0 C1D A:BCL810 3.1 65.3 1.0 C4C A:BCL810 3.1 66.7 1.0 C4A A:BCL810 3.1 66.1 1.0 C1A A:BCL810 3.2 61.6 1.0 CD2 A:HIS376 3.2 56.6 1.0 CHC A:BCL810 3.4 66.5 1.0 CHB A:BCL810 3.4 67.2 1.0 CHA A:BCL810 3.4 60.9 1.0 CHD A:BCL810 3.4 67.3 1.0 C12 A:BCL810 3.7 68.0 1.0 CE1 A:HIS376 3.9 60.2 1.0 C3D A:BCL810 4.2 65.8 1.0 C11 A:BCL810 4.2 72.7 1.0 C2D A:BCL810 4.2 68.1 1.0 C2B A:BCL810 4.2 58.0 1.0 C3B A:BCL810 4.3 64.4 1.0 C2C A:BCL810 4.3 74.3 1.0 C2A A:BCL810 4.4 59.9 1.0 C3C A:BCL810 4.4 66.7 1.0 C3A A:BCL810 4.4 64.5 1.0 CG A:HIS376 4.5 56.0 1.0 C15 A:BCL810 4.5 70.9 1.0 C13 A:BCL810 4.7 67.6 1.0 ND1 A:HIS376 4.8 60.8 1.0 CAA A:BCL810 4.8 68.4 1.0 OD1 A:ASN290 4.8 68.3 1.0 CBD A:BCL810 4.9 61.8 1.0

R.Puskar, C.Du Truong, K.Swain, S.Chowdhury, K.Y.Chan, S.Li, K.W.Cheng, T.Y.Wang, Y.P.Poh, Y.Mazor, H.Liu, T.F.Chou, B.L.Nannenga, P.L.Chiu. Molecular Asymmetry of A Photosynthetic Supercomplex From Green Sulfur Bacteria. Nat Commun V. 13 5824 2022.
ISSN: ESSN 2041-1723
PubMed: 36192412
DOI: 10.1038/S41467-022-33505-4