Magnesium in PDB 7uo2: E.Coli Rnasep Holoenzyme with MG2+

All present enzymatic activity of E.Coli Rnasep Holoenzyme with MG2+:
3.1.26.5;

The binding sites of Magnesium atom in the E.Coli Rnasep Holoenzyme with MG2+ (pdb code 7uo2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the E.Coli Rnasep Holoenzyme with MG2+, PDB code: 7uo2:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the E.Coli Rnasep Holoenzyme with MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of E.Coli Rnasep Holoenzyme with MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:30.0 occ:1.00 OP1 B:A67 2.0 61.2 1.0 OP1 B:A351 2.1 52.7 1.0 OP2 B:A352 2.4 56.9 1.0 C5' B:A67 2.6 61.2 1.0 O5' B:A67 2.6 61.2 1.0 P B:A67 2.8 61.2 1.0 P B:A351 2.9 52.7 1.0 O3' B:G350 2.9 52.2 1.0 O5' B:A351 3.4 52.7 1.0 P B:A352 3.4 56.9 1.0 C4' B:A351 3.4 52.7 1.0 C2' B:A66 3.5 55.1 1.0 O3' B:A66 3.6 55.1 1.0 O2' B:A66 3.7 55.1 1.0 O3' B:A351 3.8 52.7 1.0 OP1 B:A352 3.8 56.9 1.0 C5' B:A351 3.8 52.7 1.0 C3' B:A66 3.9 55.1 1.0 C3' B:A351 4.1 52.7 1.0 C3' B:G350 4.1 52.2 1.0 C4' B:A67 4.1 61.2 1.0 OP2 B:A67 4.2 61.2 1.0 OP2 B:A351 4.3 52.7 1.0 C4' B:G350 4.4 52.2 1.0 O4' B:A351 4.5 52.7 1.0 O5' B:A352 4.8 56.9 1.0 O4' B:A67 4.8 61.2 1.0 C1' B:A66 4.9 55.1 1.0

Magnesium binding site 2 out of 3 in the E.Coli Rnasep Holoenzyme with MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of E.Coli Rnasep Holoenzyme with MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:30.0 occ:1.00 O2' B:A66 3.6 55.1 1.0 N6 B:A328 4.2 56.5 1.0 OP1 B:A352 4.3 56.9 1.0 OP2 B:C355 4.4 62.2 1.0 O3' B:A351 4.5 52.7 1.0 OP1 B:C354 4.5 61.2 1.0 C2' B:A66 4.9 55.1 1.0

Magnesium binding site 3 out of 3 in the E.Coli Rnasep Holoenzyme with MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of E.Coli Rnasep Holoenzyme with MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg403 b:30.0 occ:1.00 OP1 B:G250 2.0 79.4 1.0 OP1 B:A249 2.6 75.6 1.0 P B:G250 2.8 79.4 1.0 OP2 B:G250 3.0 79.4 1.0 O3' B:A249 3.5 75.6 1.0 C5' B:A249 3.6 75.6 1.0 P B:A249 3.9 75.6 1.0 O5' B:A249 4.1 75.6 1.0 C3' B:A249 4.2 75.6 1.0 C4' B:A249 4.2 75.6 1.0 O5' B:G250 4.3 79.4 1.0 O3' B:A248 4.7 70.3 1.0 OP2 B:A249 5.0 75.6 1.0

J.Zhu, W.Huang, J.Zhao, L.Huynh, D.J.Taylor, M.E.Harris. Structural and Mechanistic Basis For Recognition of Alternative Trna Precursor Substrates By Bacterial Ribonuclease P. Nat Commun V. 13 5120 2022.
ISSN: ESSN 2041-1723
PubMed: 36045135
DOI: 10.1038/S41467-022-32843-7