Magnesium in PDB 7z4l: SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State

The structure of SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Magnesium atom in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State (pdb code 7z4l). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State, PDB code: 7z4l:

Magnesium binding site 1 out of 1 in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:60.8 occ:1.00 O A:HOH301 1.8 52.0 1.0 O A:HOH302 1.8 61.5 1.0 O A:HOH304 1.9 48.0 1.0 O A:HOH305 1.9 55.1 1.0 O A:HOH303 1.9 53.4 1.0 OP1 A:U50 2.0 44.4 1.0 P A:U50 3.3 55.8 1.0 O6 A:G62 3.7 48.8 1.0 OP1 A:A51 3.9 37.8 1.0 OP2 A:U50 3.9 46.7 1.0 O5' A:U50 3.9 40.0 1.0 C5' A:U50 4.3 31.0 1.0 O6 A:G53 4.4 41.3 1.0 OP1 A:A52 4.4 40.8 1.0 O3' A:A49 4.5 43.3 1.0 C6 A:G62 4.7 40.8 1.0 N1 A:G62 4.8 38.8 1.0 OP2 A:A52 4.8 39.7 1.0 NZ B:LYS1123 5.0 51.7 1.0 OG1 B:THR73 5.0 73.8 1.0

M.Pacesa, L.Loeff, I.Querques, L.M.Muckenfuss, M.Sawicka, M.Jinek. R-Loop Formation and Conformational Activation Mechanisms of CAS9. Nature V. 609 191 2022.
ISSN: ESSN 1476-4687
PubMed: 36002571
DOI: 10.1038/S41586-022-05114-0