Magnesium in PDB 7zz4: Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa

All present enzymatic activity of Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa:
6.4.1.1;

The binding sites of Magnesium atom in the Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa (pdb code 7zz4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa, PDB code: 7zz4:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of "Bc Closed" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1202 b:91.3 occ:1.00 O1G A:ATP1201 2.0 87.0 1.0 OE2 A:GLU286 2.1 64.8 1.0 O3A A:ATP1201 2.1 87.4 1.0 O3B A:ATP1201 2.2 93.2 1.0 O1A A:ATP1201 2.2 86.3 1.0 PB A:ATP1201 2.4 96.8 1.0 PA A:ATP1201 2.5 94.1 1.0 PG A:ATP1201 2.5 108.2 1.0 O1B A:ATP1201 2.8 90.7 1.0 O5' A:ATP1201 3.2 88.7 1.0 OE1 A:GLU274 3.2 79.0 1.0 CD A:GLU286 3.3 61.3 1.0 O3G A:ATP1201 3.6 90.9 1.0 O2G A:ATP1201 3.7 94.3 1.0 C5' A:ATP1201 3.7 84.8 1.0 O2B A:ATP1201 3.8 86.1 1.0 O2A A:ATP1201 3.9 91.1 1.0 OE1 A:GLU286 3.9 63.9 1.0 OE2 A:GLU274 3.9 73.9 1.0 CD A:GLU274 4.0 72.0 1.0 CG A:GLU286 4.4 49.2 1.0 NZ A:LYS236 4.4 81.2 1.0 NE2 A:HIS207 4.6 71.3 1.0

J.P.Lopez-Alonso, M.Lazaro, D.Gil-Carton, P.H.Choi, L.Tong, M.Valle. Cryoem Structural Exploration of Catalytically Active Enzyme Pyruvate Carboxylase. Nat Commun V. 13 6185 2022.
ISSN: ESSN 2041-1723
PubMed: 36261450
DOI: 10.1038/S41467-022-33987-2