Magnesium in PDB 8b4h: Ista Transposase Cleaved Donor Complex

The binding sites of Magnesium atom in the Ista Transposase Cleaved Donor Complex (pdb code 8b4h). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Ista Transposase Cleaved Donor Complex, PDB code: 8b4h:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Ista Transposase Cleaved Donor Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ista Transposase Cleaved Donor Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg700 b:35.9 occ:1.00 O3' F:DA55 2.5 27.0 1.0 HO3' F:DA55 2.8 27.0 1.0 H4' F:DA55 2.8 27.0 1.0 OD2 A:ASP124 2.9 23.5 1.0 OD1 A:ASP124 3.0 23.5 1.0 OE2 A:GLU233 3.2 26.6 1.0 O5' F:DA55 3.3 27.0 1.0 C4' F:DA55 3.4 27.0 1.0 CG A:ASP124 3.4 23.5 1.0 C3' F:DA55 3.5 27.0 1.0 H5' F:DA55 3.5 27.0 1.0 C5' F:DA55 3.6 27.0 1.0 OD2 A:ASP187 3.7 39.0 1.0 H3' F:DA55 4.1 27.0 1.0 CD A:GLU233 4.1 26.6 1.0 O A:TRP125 4.2 28.8 1.0 OE1 A:GLU233 4.3 26.6 1.0 H2'' F:DA55 4.3 27.0 1.0 HD2 A:LYS229 4.3 29.2 1.0 H A:TRP125 4.5 28.8 1.0 C2' F:DA55 4.5 27.0 1.0 H5'' F:DA55 4.6 27.0 1.0 HB2 A:ASP187 4.6 39.0 1.0 HB1 A:ALA226 4.7 34.1 1.0 O4' F:DA55 4.7 27.0 1.0 P F:DA55 4.7 27.0 1.0 CG A:ASP187 4.8 39.0 1.0 CB A:ASP124 4.9 23.5 1.0 OP1 F:DA55 4.9 27.0 1.0 HB3 A:ASP187 5.0 39.0 1.0

Magnesium binding site 2 out of 2 in the Ista Transposase Cleaved Donor Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Ista Transposase Cleaved Donor Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg700 b:33.8 occ:1.00 O3' H:DA55 2.5 23.4 1.0 HO3' H:DA55 2.8 23.4 1.0 H4' H:DA55 2.8 23.4 1.0 OD2 B:ASP124 2.9 21.3 1.0 OD1 B:ASP124 3.0 21.3 1.0 OE2 B:GLU233 3.2 29.3 1.0 O5' H:DA55 3.3 23.4 1.0 C4' H:DA55 3.4 23.4 1.0 CG B:ASP124 3.4 21.3 1.0 C3' H:DA55 3.5 23.4 1.0 H5' H:DA55 3.5 23.4 1.0 C5' H:DA55 3.6 23.4 1.0 OD2 B:ASP187 3.7 38.1 1.0 H3' H:DA55 4.1 23.4 1.0 CD B:GLU233 4.1 29.3 1.0 O B:TRP125 4.2 27.5 1.0 OE1 B:GLU233 4.3 29.3 1.0 H2'' H:DA55 4.3 23.4 1.0 HD2 B:LYS229 4.3 28.3 1.0 H B:TRP125 4.5 27.5 1.0 C2' H:DA55 4.5 23.4 1.0 H5'' H:DA55 4.6 23.4 1.0 HB2 B:ASP187 4.6 38.1 1.0 HB1 B:ALA226 4.7 32.8 1.0 O4' H:DA55 4.7 23.4 1.0 P H:DA55 4.7 23.4 1.0 CG B:ASP187 4.8 38.1 1.0 CB B:ASP124 4.9 21.3 1.0 OP1 H:DA55 4.9 23.4 1.0 HB3 B:ASP187 5.0 38.1 1.0

M.Spinola-Amilibia, L.Araujo-Bazan, A.De La Gandara, J.M.Berger, E.Arias-Palomo. IS21 Family Transposase Cleaved Donor Complex Traps Two Right-Handed Superhelical Crossings. Nat Commun V. 14 2335 2023.
ISSN: ESSN 2041-1723
PubMed: 37087515
DOI: 10.1038/S41467-023-38071-X