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Magnesium in PDB 8bek: Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position

Enzymatic activity of Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position

All present enzymatic activity of Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position:
2.7.7.48;

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position (pdb code 8bek). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position, PDB code: 8bek:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 8bek

Go back to Magnesium Binding Sites List in 8bek
Magnesium binding site 1 out of 2 in the Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg801

b:52.3
occ:1.00
OE2 A:GLU80 2.8 62.6 1.0
OD1 A:ASP108 3.0 65.5 1.0
OE2 A:GLU119 3.3 75.2 1.0
O A:LEU106 3.8 58.3 1.0
CG A:ASP108 3.8 63.5 1.0
CD A:GLU80 4.0 64.3 1.0
OD2 A:ASP108 4.0 66.1 1.0
CD A:GLU119 4.4 76.4 1.0
CE1 A:HIS41 4.6 66.3 1.0
OE1 A:GLU119 4.6 73.5 1.0
CG A:GLU80 4.8 63.6 1.0
OE1 A:GLU80 4.8 61.5 1.0
NE2 A:HIS41 4.9 66.4 1.0

Magnesium binding site 2 out of 2 in 8bek

Go back to Magnesium Binding Sites List in 8bek
Magnesium binding site 2 out of 2 in the Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Early Transcription Elongation State of Influenza A/H7N9 Backtracked Polymerase with Singly Incoporated T1106 at the U +1 Position within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg801

b:42.4
occ:1.00
O B:HOH905 1.8 26.4 1.0
O B:HOH919 1.8 17.6 1.0
OD1 B:ASP446 1.8 32.4 1.0
OD1 B:ASP305 2.0 34.2 1.0
O B:GLY304 2.6 16.1 1.0
CG B:ASP446 2.6 27.5 1.0
OD2 B:ASP446 2.9 31.8 1.0
CG B:ASP305 3.2 32.6 1.0
C B:GLY304 3.6 15.1 1.0
OD2 B:ASP305 3.9 29.1 1.0
CB B:ASP446 3.9 19.9 1.0
OE1 B:GLU491 4.0 31.6 1.0
CA B:ASP305 4.2 23.0 1.0
N B:ASP305 4.2 16.2 1.0
CB B:ASP305 4.3 29.0 1.0
CA B:ASP446 4.3 19.1 1.0
N B:PHE447 4.4 14.8 1.0
N B:GLY304 4.5 18.6 1.0
O B:PHE447 4.5 30.0 1.0
CA B:GLY304 4.6 22.0 1.0
CG2 B:THR303 4.8 18.3 1.0
C B:ASP446 4.9 21.4 1.0
O M:HOH106 4.9 18.4 1.0
O B:GLU491 5.0 29.6 1.0

Reference:

T.Kouba, S.Cusack. Direct Observation of Backtracking By Influenza A and B Polymerases Upon Consecutive Incorporation of Nucleoside Analogue T1106 Cell Rep 2022.
ISSN: ESSN 2211-1247
DOI: 10.1016/J.CELREP.2022.111901
Page generated: Thu Oct 3 19:21:36 2024

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