Magnesium in PDB 8bwo: Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation)

All present enzymatic activity of Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation):
7.6.2.2; 7.6.2.3;

The binding sites of Magnesium atom in the Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation) (pdb code 8bwo). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation), PDB code: 8bwo:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1403 b:79.9 occ:1.00 O3G A:ATP1402 1.9 79.2 1.0 O1B A:ATP1402 1.9 79.2 1.0 OE1 A:GLN480 2.4 74.7 1.0 PG A:ATP1402 2.4 79.2 1.0 O1G A:ATP1402 2.4 79.2 1.0 PB A:ATP1402 2.8 79.2 1.0 O3B A:ATP1402 2.8 79.2 1.0 CD A:GLN480 3.1 74.7 1.0 NE2 A:GLN480 3.1 74.7 1.0 O3A A:ATP1402 3.8 79.2 1.0 N A:VAL1179 3.9 79.6 1.0 O2G A:ATP1402 3.9 79.2 1.0 O2B A:ATP1402 3.9 79.2 1.0 CB A:SER452 4.0 72.2 1.0 CB A:SER1178 4.1 81.0 1.0 CB A:VAL1179 4.2 79.6 1.0 O1A A:ATP1402 4.3 79.2 1.0 PA A:ATP1402 4.4 79.2 1.0 CG2 A:VAL1179 4.4 79.6 1.0 N A:GLY1180 4.5 79.3 1.0 CG A:GLN480 4.5 74.7 1.0 CA A:VAL1179 4.6 79.6 1.0 CA A:SER1178 4.6 81.0 1.0 C A:SER1178 4.7 81.0 1.0 OG A:SER452 4.9 72.2 1.0 N A:SER452 4.9 72.2 1.0 OG A:SER1178 5.0 81.0 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Nanodisc-Reconstituted Human MRP4 with E1202Q Mutation (Outward-Facing Occluded Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1404 b:75.2 occ:1.00 OG A:SER1082 1.9 72.0 1.0 OE1 A:GLN1122 2.0 80.3 1.0 O2B A:ATP1401 2.3 79.3 1.0 OE1 A:GLN1202 2.7 80.4 1.0 O3G A:ATP1401 2.8 79.3 1.0 CD A:GLN1122 2.9 80.3 1.0 CB A:SER1082 3.0 72.0 1.0 NE2 A:GLN1122 3.4 80.3 1.0 PB A:ATP1401 3.5 79.3 1.0 OD1 A:ASP1201 3.9 78.4 1.0 OD2 A:ASP1201 3.9 78.4 1.0 CD A:GLN1202 3.9 80.4 1.0 PG A:ATP1401 4.0 79.3 1.0 O3B A:ATP1401 4.0 79.3 1.0 CG A:GLN1122 4.2 80.3 1.0 CA A:SER1082 4.2 72.0 1.0 CG A:ASP1201 4.3 78.4 1.0 CB A:GLN1122 4.3 80.3 1.0 N A:SER1082 4.4 72.0 1.0 O1B A:ATP1401 4.5 79.3 1.0 O3A A:ATP1401 4.6 79.3 1.0 NE2 A:GLN1202 4.6 80.4 1.0 O1G A:ATP1401 4.6 79.3 1.0 N A:GLY537 4.6 77.1 1.0 PA A:ATP1401 4.8 79.3 1.0 O1A A:ATP1401 4.8 79.3 1.0

M.Bloch, I.Raj, T.H.Pape, N.M.I.Taylor. Structural and Mechanistic Basis of Substrate Transport By the Multidrug Transporter MRP4 To Be Published.