Magnesium in PDB 8cag: Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli

All present enzymatic activity of Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli:
2.4.2.8;

The structure of Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli, PDB code: 8cag was solved by V.I.Timofeev, M.B.Shevtsov, Y.A.Abramchik, M.A.Kostromina, E.A.Zayats, I.P.Kuranova, R.S.Esipov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.74 / 2.40
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	84.65, 84.65, 167.242, 90, 90, 120
R / Rfree (%)	25.4 / 29.3

The binding sites of Magnesium atom in the Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli (pdb code 8cag). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli, PDB code: 8cag:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:34.7 occ:1.00 O A:HOH315 3.2 38.3 1.0 N A:GLY109 3.6 40.5 1.0 CA A:GLY109 3.8 40.5 1.0 C A:GLY109 4.0 40.7 1.0 N A:ASN110 4.1 43.1 1.0 N A:THR111 4.1 43.9 1.0 CB A:THR111 4.2 40.2 1.0 N A:LEU112 4.2 44.4 1.0 OG A:SER108 4.2 44.4 1.0 OD1 A:ASP107 4.3 40.4 1.0 CB A:LEU112 4.4 50.4 1.0 N A:ASP107 4.4 41.8 1.0 OG1 A:THR111 4.5 37.1 1.0 O A:ILE105 4.6 30.9 1.0 CA A:THR111 4.6 42.5 1.0 CA A:ILE106 4.6 41.8 1.0 N A:SER108 4.7 41.8 1.0 O A:GLY109 4.7 38.9 1.0 C A:SER108 4.8 44.5 1.0 CG2 A:ILE106 4.8 39.3 1.0 C A:ASN110 5.0 48.1 1.0 C A:THR111 5.0 44.1 1.0 CA A:LEU112 5.0 46.0 1.0 CD2 A:LEU112 5.0 62.5 1.0

Magnesium binding site 2 out of 2 in the Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:40.8 occ:1.00 N B:GLY109 3.6 40.9 1.0 CA B:GLY109 3.8 37.4 1.0 O B:HOH318 3.8 45.5 1.0 N B:ASN110 3.9 39.9 1.0 C B:GLY109 4.0 34.1 1.0 N B:THR111 4.0 43.1 1.0 N B:LEU112 4.1 46.6 1.0 CB B:THR111 4.1 40.2 1.0 N B:ASP107 4.2 50.8 1.0 OG B:SER108 4.4 55.4 1.0 CB B:LEU112 4.5 53.2 1.0 OG1 B:THR111 4.5 38.9 1.0 CA B:THR111 4.5 41.8 1.0 N B:SER108 4.6 41.9 1.0 O B:GLY109 4.6 36.1 1.0 CA B:ILE106 4.6 48.6 1.0 O B:ILE105 4.7 43.9 1.0 CB B:ASP107 4.7 65.3 1.0 C B:SER108 4.8 41.3 1.0 C B:THR111 4.9 38.5 1.0 C B:ASN110 4.9 44.4 1.0 CG2 B:ILE106 4.9 47.6 1.0 CA B:ASP107 4.9 51.6 1.0 CA B:ASN110 4.9 44.5 1.0 CA B:LEU112 5.0 46.5 1.0 C B:ILE106 5.0 49.0 1.0 CD2 B:LEU112 5.0 58.7 1.0

V.I.Timofeev, M.B.Shevtsov, Y.A.Abramchik, M.A.Kostromina, E.A.Zayats, I.P.Kuranova, R.S.Esipov. Hypoxanthine-Guanine Phosphoribosyltransferase From E. Coli To Be Published.