Magnesium in PDB 8dba: Crystal Structure of Dodecameric Kaic

The structure of Crystal Structure of Dodecameric Kaic, PDB code: 8dba was solved by R.A.P.Padua, T.Grant, W.Pitsawong, M.S.Hoemberger, R.Otten, N.Bradshaw, N.Grigorieff, D.Kern, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.10 / 3.50
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	105.11, 136.09, 146.2, 93.13, 94.43, 108.09
R / Rfree (%)	23.6 / 27.9

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 23;

Binding sites:

The binding sites of Magnesium atom in the Crystal Structure of Dodecameric Kaic (pdb code 8dba). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 23 binding sites of Magnesium where determined in the Crystal Structure of Dodecameric Kaic, PDB code: 8dba:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:76.0 occ:1.00 O A:HOH701 2.1 48.6 1.0 OE2 A:GLU302 2.8 97.3 1.0 OG C:SER414 2.9 82.6 1.0 HZ1 A:LYS278 2.9 80.7 1.0 O2B A:ADP604 2.9 93.1 1.0 HG C:SER414 2.9 100.0 1.0 O3B A:ADP604 3.0 77.4 1.0 HE2 A:LYS278 3.1 69.7 1.0 O C:HOH703 3.2 49.6 1.0 PB A:ADP604 3.2 84.0 1.0 HH21 C:ARG441 3.2 92.9 1.0 HH22 C:ARG441 3.3 92.9 1.0 NZ A:LYS278 3.5 66.5 1.0 O1B A:ADP604 3.6 69.1 1.0 NH2 C:ARG441 3.6 76.7 1.0 HD13 A:LEU399 3.6 75.2 1.0 CE A:LYS278 3.6 58.6 1.0 HZ3 A:LYS278 3.7 80.7 1.0 HE3 A:LYS278 3.8 69.7 1.0 CD A:GLU302 3.8 91.9 1.0 HG A:SER279 4.0 70.8 1.0 OG A:SER279 4.1 61.1 1.0 OE1 A:GLU302 4.2 82.5 1.0 CB C:SER414 4.2 82.0 1.0 HA C:SER414 4.2 111.6 1.0 CD1 A:LEU399 4.2 62.7 1.0 HD12 A:LEU399 4.2 75.2 1.0 HZ2 A:LYS278 4.3 80.7 1.0 HD11 A:LEU399 4.3 75.2 1.0 HB2 A:LYS278 4.3 80.2 1.0 CG A:PRO363 4.4 61.8 1.0 HB2 C:SER414 4.5 99.3 1.0 HA A:ALA274 4.6 73.7 1.0 H A:SER279 4.7 81.9 1.0 CA C:SER414 4.7 92.3 1.0 O3A A:ADP604 4.8 76.2 1.0 HB3 C:SER414 4.8 99.3 1.0 OD2 A:ASP362 4.8 73.4 1.0 CZ C:ARG441 4.9 85.8 1.0 O C:SER414 4.9 89.2 1.0 HG1 A:THR397 4.9 80.5 1.0

Magnesium binding site 2 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:98.8 occ:1.00 O A:HOH704 2.4 96.4 1.0 O A:HOH702 2.5 88.2 1.0 OG1 A:THR38 2.6 90.7 1.0 O2A A:ADP601 3.0 115.9 1.0 HB A:THR38 3.0 124.1 1.0 HG21 A:THR38 3.2 147.7 1.0 HG1 A:THR38 3.2 109.7 1.0 O2B A:ADP601 3.2 95.2 1.0 CB A:THR38 3.3 102.7 1.0 O3A A:ADP601 3.3 122.5 1.0 PA A:ADP601 3.6 122.6 1.0 O A:HOH705 3.7 91.2 1.0 CG2 A:THR38 3.8 122.3 1.0 OE2 A:GLU63 3.9 134.5 1.0 PB A:ADP601 3.9 112.3 1.0 NH2 C:ARG209 3.9 126.2 1.0 HH22 C:ARG209 3.9 152.3 1.0 HH21 C:ARG209 4.0 152.3 1.0 O1A A:ADP601 4.1 108.8 1.0 O A:HOH703 4.1 93.7 1.0 CZ C:ARG209 4.2 140.3 1.0 H A:THR38 4.3 111.4 1.0 HG22 A:THR38 4.3 147.7 1.0 O1B A:ADP601 4.4 104.8 1.0 HG23 A:THR38 4.4 147.7 1.0 NH1 C:ARG209 4.5 144.7 1.0 HH12 C:ARG209 4.5 174.6 1.0 CA A:THR38 4.7 93.5 1.0 NE C:ARG209 4.7 144.1 1.0 HE C:ARG209 4.8 173.8 1.0 CD A:GLU63 4.9 144.7 1.0 OD2 A:ASP130 4.9 102.5 1.0 N A:THR38 4.9 92.1 1.0 HH11 C:ARG209 5.0 174.6 1.0

Magnesium binding site 3 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg602 b:36.8 occ:1.00 O B:HOH703 2.1 42.5 1.0 O B:HOH701 2.7 38.7 1.0 O3B B:ADP604 2.8 86.9 1.0 OG B:SER279 2.8 52.7 1.0 O1B B:ADP604 3.1 69.3 1.0 OE2 B:GLU302 3.1 87.6 1.0 OD1 B:ASP362 3.2 52.0 1.0 PB B:ADP604 3.3 78.7 1.0 HE2 B:LYS278 3.4 91.0 1.0 OD2 B:ASP362 3.7 81.2 1.0 CG B:ASP362 3.8 67.5 1.0 HZ3 B:LYS278 3.9 103.8 1.0 O2B B:ADP604 3.9 61.0 1.0 H B:SER279 4.0 59.2 1.0 HB2 B:LYS278 4.1 68.5 1.0 HG1 B:THR397 4.1 81.3 1.0 HB3 B:PRO363 4.1 76.9 1.0 HZ2 B:LYS278 4.2 103.8 1.0 HD21 B:LEU399 4.2 89.4 1.0 HG3 B:PRO363 4.2 76.4 1.0 CE B:LYS278 4.2 75.1 1.0 CD B:GLU302 4.2 80.9 1.0 CB B:SER279 4.2 58.8 1.0 NZ B:LYS278 4.3 85.7 1.0 HB2 A:SER414 4.5 117.8 1.0 HB2 B:SER279 4.5 71.5 1.0 HE3 B:LYS278 4.6 91.0 1.0 N B:SER279 4.6 51.3 1.0 O3A B:ADP604 4.7 59.4 1.0 HB3 B:SER279 4.7 71.5 1.0 HH11 A:ARG441 4.8 101.1 1.0 OG1 B:THR397 4.8 67.0 1.0 OE1 B:GLU302 4.8 88.9 1.0 HG B:LEU399 4.8 94.7 1.0 HB B:THR397 4.9 62.1 1.0 HD3 B:PRO363 4.9 79.7 1.0 CG B:PRO363 4.9 63.0 1.0 CB B:PRO363 4.9 63.4 1.0 CA B:SER279 5.0 59.4 1.0 HH12 A:ARG441 5.0 101.1 1.0

Magnesium binding site 4 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg603 b:65.3 occ:1.00 OG1 B:THR38 2.7 88.3 1.0 O B:HOH702 2.7 65.7 1.0 HG1 B:THR38 2.8 106.8 1.0 O2B B:ADP601 2.9 113.7 1.0 OE1 B:GLU63 3.3 108.4 1.0 HB B:THR38 3.3 109.2 1.0 HG22 B:THR38 3.4 107.8 1.0 OE2 B:GLU63 3.5 133.1 1.0 CB B:THR38 3.5 90.2 1.0 CD B:GLU63 3.6 120.7 1.0 O3A B:ADP601 3.6 118.0 1.0 PB B:ADP601 3.7 111.6 1.0 O B:HOH704 3.8 82.2 1.0 O2A B:ADP601 3.9 123.9 1.0 O3B B:ADP601 4.0 106.1 1.0 CG2 B:THR38 4.0 89.1 1.0 PA B:ADP601 4.3 126.1 1.0 NH2 A:ARG209 4.4 125.1 1.0 OD1 B:ASP130 4.4 100.6 1.0 HG3 B:GLU63 4.5 143.5 1.0 H B:THR38 4.5 110.6 1.0 OD2 B:ASP130 4.6 119.8 1.0 NE A:ARG209 4.6 117.3 1.0 HG23 B:THR38 4.6 107.8 1.0 HG21 B:THR38 4.6 107.8 1.0 CZ A:ARG209 4.6 130.7 1.0 CG B:GLU63 4.7 118.8 1.0 O1A B:ADP601 4.8 90.2 1.0 CA B:THR38 4.8 90.4 1.0 HG21 B:THR166 4.9 115.6 1.0 HD2 A:LYS207 4.9 131.6 1.0 CG B:ASP130 4.9 118.2 1.0 HZ3 A:LYS207 5.0 139.9 1.0

Magnesium binding site 5 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg605 b:84.0 occ:1.00 O F:HOH705 2.5 89.1 1.0 OG1 F:THR38 2.8 91.0 1.0 HG21 F:THR38 3.0 112.2 1.0 HB F:THR38 3.0 106.3 1.0 NH1 B:ARG209 3.2 124.2 1.0 CB F:THR38 3.3 87.8 1.0 CD B:ARG209 3.3 119.1 1.0 HG2 F:GLU63 3.4 141.8 1.0 O F:HOH706 3.4 60.2 1.0 O2A F:ADP601 3.5 117.5 1.0 CZ B:ARG209 3.6 123.3 1.0 NE B:ARG209 3.6 126.1 1.0 CG2 F:THR38 3.6 92.8 1.0 O1B F:ADP601 3.6 99.4 1.0 O3A F:ADP601 3.7 101.3 1.0 OE2 F:GLU63 3.7 130.7 1.0 O F:HOH703 3.9 107.3 1.0 PA F:ADP601 4.0 116.3 1.0 CD F:GLU63 4.0 124.7 1.0 PB F:ADP601 4.1 110.9 1.0 OD2 F:ASP130 4.1 96.5 1.0 O F:HOH704 4.2 65.1 1.0 CG F:GLU63 4.2 117.5 1.0 O2B F:ADP601 4.2 104.9 1.0 HG22 F:THR38 4.2 112.2 1.0 HG23 F:THR38 4.3 112.2 1.0 O1A F:ADP601 4.3 112.8 1.0 NH2 B:ARG209 4.5 114.9 1.0 H F:THR38 4.6 105.9 1.0 OE1 F:GLU63 4.7 120.7 1.0 HG3 F:GLU63 4.7 141.8 1.0 CG B:ARG209 4.8 115.4 1.0 CA F:THR38 4.8 83.1 1.0 HD2 B:LYS207 4.8 132.9 1.0 HZ2 B:LYS207 4.9 125.0 1.0

Magnesium binding site 6 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg602 b:63.1 occ:1.00 O C:HOH701 2.0 54.9 1.0 O2B C:ADP604 2.5 97.3 1.0 PB C:ADP604 3.2 106.5 1.0 OG C:SER279 3.2 67.2 1.0 HH12 E:ARG441 3.3 112.2 1.0 O3B C:ADP604 3.4 81.0 1.0 HG C:SER279 3.5 81.6 1.0 HB2 E:SER414 3.6 119.8 1.0 O1B C:ADP604 3.6 101.4 1.0 OD1 C:ASP362 3.6 86.7 1.0 NH1 E:ARG441 4.1 92.8 1.0 HH22 E:ARG441 4.2 93.6 1.0 HZ3 C:LYS278 4.2 107.5 1.0 OD2 C:ASP362 4.3 80.1 1.0 CB E:SER414 4.4 99.1 1.0 HB3 E:SER414 4.4 119.8 1.0 CG C:ASP362 4.4 78.8 1.0 HA E:SER414 4.4 120.8 1.0 HG3 C:PRO363 4.4 88.6 1.0 HB3 C:PRO363 4.5 77.9 1.0 HH11 E:ARG441 4.5 112.2 1.0 HD3 C:PRO363 4.5 106.4 1.0 CB C:SER279 4.5 67.9 1.0 HB2 C:SER279 4.6 82.4 1.0 HE2 C:LYS278 4.6 103.6 1.0 H C:SER279 4.6 95.2 1.0 O E:SER414 4.6 92.2 1.0 O3A C:ADP604 4.7 74.2 1.0 HZ2 C:LYS278 4.7 107.5 1.0 OE1 C:GLU303 4.8 92.0 1.0 HB2 C:GLU302 4.8 126.0 1.0 NZ C:LYS278 4.8 88.8 1.0 NH2 E:ARG441 4.9 77.3 1.0 CA E:SER414 4.9 100.0 1.0 HZ1 E:LYS439 4.9 88.8 1.0 CZ E:ARG441 4.9 88.6 1.0 O C:HOH702 4.9 77.2 1.0 HB3 C:SER279 4.9 82.4 1.0 O1A C:ADP604 5.0 98.8 1.0 OE1 C:GLU302 5.0 115.5 1.0

Magnesium binding site 7 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg603 b:98.3 occ:1.00 O C:HOH707 2.0 97.3 1.0 O C:HOH706 2.4 94.0 1.0 O1A C:ADP601 2.7 112.4 1.0 HG1 C:THR38 2.8 112.1 1.0 O C:HOH704 3.1 77.0 1.0 O3B C:ADP601 3.5 104.5 1.0 O1B C:ADP601 3.5 100.5 1.0 PA C:ADP601 3.6 103.4 1.0 OG1 C:THR38 3.6 92.6 1.0 PB C:ADP601 3.8 101.4 1.0 O3A C:ADP601 3.8 104.0 1.0 HB C:THR38 3.8 111.5 1.0 HG21 C:THR38 3.9 111.5 1.0 OE1 C:GLU63 3.9 116.6 1.0 O2A C:ADP601 4.0 83.4 1.0 HZ3 E:LYS207 4.1 154.8 1.0 CB C:THR38 4.2 92.2 1.0 HD2 E:LYS207 4.3 120.7 1.0 CG2 C:THR38 4.5 92.2 1.0 CD C:GLU63 4.6 125.3 1.0 OE2 C:GLU63 4.6 120.6 1.0 OD2 C:ASP130 4.7 113.9 1.0 NZ E:LYS207 5.0 128.3 1.0 HD22 C:ASN71 5.0 116.5 1.0

Magnesium binding site 8 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg602 b:85.8 occ:1.00 O2B D:ADP604 1.9 92.5 1.0 O D:ALA274 2.4 83.1 1.0 O3B D:ADP604 2.5 75.9 1.0 PB D:ADP604 2.5 106.7 1.0 HZ1 F:LYS439 2.5 88.3 1.0 HE3 F:LYS439 2.6 95.2 1.0 HE2 F:LYS439 2.9 95.2 1.0 CE F:LYS439 3.0 78.6 1.0 O3A D:ADP604 3.1 72.6 1.0 NZ F:LYS439 3.1 72.8 1.0 C D:ALA274 3.2 73.0 1.0 HZ1 D:LYS278 3.3 81.1 1.0 HA2 D:GLY275 3.5 70.4 1.0 HA D:ALA274 3.6 72.2 1.0 HA F:SER414 3.7 100.9 1.0 HZ2 F:LYS439 3.7 88.3 1.0 HZ3 F:LYS439 3.7 88.3 1.0 HB1 D:ALA274 3.7 74.1 1.0 HH22 F:ARG441 3.8 98.9 1.0 HZ3 D:LYS278 3.8 81.1 1.0 O D:HOH702 3.9 44.1 1.0 CA D:ALA274 3.9 63.9 1.0 O1B D:ADP604 3.9 94.0 1.0 NZ D:LYS278 4.0 66.8 1.0 N D:GLY275 4.1 64.7 1.0 HH12 F:ARG441 4.1 117.8 1.0 NH2 F:ARG441 4.2 81.7 1.0 HZ2 D:LYS278 4.2 81.1 1.0 CA D:GLY275 4.2 62.4 1.0 CB D:ALA274 4.3 65.1 1.0 O2A D:ADP604 4.3 88.9 1.0 PA D:ADP604 4.4 64.4 1.0 OE2 D:GLU302 4.4 103.7 1.0 OG F:SER414 4.5 80.1 1.0 CD F:LYS439 4.5 73.3 1.0 NH1 F:ARG441 4.5 97.4 1.0 O D:HOH701 4.5 44.6 1.0 HH21 F:ARG441 4.6 98.9 1.0 CZ F:ARG441 4.6 89.7 1.0 CA F:SER414 4.6 83.4 1.0 HD2 F:LYS439 4.7 88.9 1.0 H D:ALA276 4.7 68.5 1.0 H D:GLY275 4.8 78.5 1.0 HA3 D:GLY275 4.8 70.4 1.0 HB2 F:SER414 4.9 112.2 1.0 HD3 F:LYS439 4.9 88.9 1.0 HB2 F:LYS439 4.9 76.5 1.0 HB3 D:ALA274 4.9 74.1 1.0 CB F:SER414 4.9 92.8 1.0 HB2 D:ALA274 5.0 74.1 1.0 O F:SER414 5.0 100.1 1.0

Magnesium binding site 9 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg603 b:68.1 occ:1.00 O D:HOH703 2.2 43.2 1.0 OG1 D:THR38 2.3 58.9 1.0 HH12 F:ARG209 2.4 122.8 1.0 HH22 F:ARG209 2.5 121.5 1.0 NH1 F:ARG209 2.8 101.6 1.0 NH2 F:ARG209 2.9 100.5 1.0 CZ F:ARG209 3.1 105.2 1.0 HG21 D:THR38 3.1 105.8 1.0 O1B D:ADP601 3.3 83.5 1.0 CB D:THR38 3.3 69.5 1.0 HH11 F:ARG209 3.4 122.8 1.0 HB D:THR38 3.4 84.3 1.0 OD1 D:ASP130 3.5 96.8 1.0 HH21 F:ARG209 3.5 121.5 1.0 CG2 D:THR38 3.7 87.4 1.0 O D:HOH705 3.7 54.9 1.0 OD2 D:ASP130 3.9 82.2 1.0 O D:HOH704 3.9 72.6 1.0 CG D:ASP130 4.0 89.5 1.0 HG23 D:THR38 4.1 105.8 1.0 PB D:ADP601 4.2 85.6 1.0 NE F:ARG209 4.2 114.0 1.0 OE1 D:GLU63 4.3 100.7 1.0 O3B D:ADP601 4.3 90.5 1.0 O3A D:ADP601 4.3 87.2 1.0 HG22 D:THR38 4.4 105.8 1.0 H D:THR38 4.5 91.5 1.0 HG3 D:GLU63 4.6 138.6 1.0 CD D:GLU63 4.6 107.6 1.0 CA D:THR38 4.6 74.5 1.0 HE F:ARG209 4.6 137.6 1.0 O1A D:ADP601 4.7 101.7 1.0 HG1 D:THR131 4.7 137.2 1.0 HD3 F:ARG209 4.7 128.3 1.0 O D:HOH706 4.8 76.2 1.0 HA D:THR38 4.8 90.3 1.0 HZ1 F:LYS207 4.9 117.8 1.0 OG1 D:THR131 4.9 113.6 1.0 N D:THR38 5.0 75.5 1.0 PA D:ADP601 5.0 90.0 1.0

Magnesium binding site 10 out of 23 in the Crystal Structure of Dodecameric Kaic


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Crystal Structure of Dodecameric Kaic within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg602 b:46.3 occ:1.00 O1B E:ADP604 2.4 78.0 1.0 O E:HOH705 2.4 41.9 1.0 OG E:SER279 2.5 65.5 1.0 O2B E:ADP604 2.9 75.6 1.0 HH11 D:ARG441 3.0 113.7 1.0 HG E:SER279 3.0 79.5 1.0 PB E:ADP604 3.1 74.8 1.0 HH12 D:ARG441 3.2 113.7 1.0 NH1 D:ARG441 3.2 94.0 1.0 OE1 E:GLU302 3.5 105.3 1.0 HB2 E:SER279 3.5 85.5 1.0 CB E:SER279 3.6 70.5 1.0 O E:HOH702 3.6 86.6 1.0 O1A E:ADP604 3.6 96.5 1.0 OD2 E:ASP362 3.8 90.6 1.0 HD2 D:ARG441 3.9 104.4 1.0 HB3 E:SER279 4.0 85.5 1.0 H E:SER279 4.0 79.6 1.0 O3A E:ADP604 4.1 65.4 1.0 OD1 E:ASP362 4.1 80.2 1.0 OE1 E:GLU303 4.1 114.2 1.0 CZ D:ARG441 4.1 100.1 1.0 PA E:ADP604 4.2 82.3 1.0 O2A E:ADP604 4.4 77.4 1.0 O3B E:ADP604 4.4 61.6 1.0 CG E:ASP362 4.4 86.8 1.0 CD E:GLU302 4.5 103.3 1.0 N E:SER279 4.7 65.6 1.0 CD D:ARG441 4.7 86.2 1.0 HZ1 D:LYS439 4.7 93.0 1.0 NE D:ARG441 4.7 99.1 1.0 CA E:SER279 4.7 64.4 1.0 CD E:GLU303 4.8 103.2 1.0 HH22 D:ARG441 4.8 102.0 1.0 HB3 E:GLU302 4.8 109.6 1.0 NH2 D:ARG441 4.9 84.3 1.0 HE3 D:LYS439 5.0 98.5 1.0 HG2 E:GLU303 5.0 118.0 1.0 HD3 D:ARG441 5.0 104.4 1.0

W.Pitsawong, R.A.P.Padua, T.Grant, M.S.Hoemberger, R.Otten, N.Bradshaw, N.Grigorieff, D.Kern. From Primordial Clocks to Circadian Oscillators Nature 2023.
ISSN: ESSN 1476-4687
DOI: 10.1038/S41586-023-05836-9