Magnesium in PDB 8elk: Hras R97F Crystal Form 1

All present enzymatic activity of Hras R97F Crystal Form 1:
3.6.5.2;

The structure of Hras R97F Crystal Form 1, PDB code: 8elk was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.07 / 1.80
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.147, 88.147, 133.27, 90, 90, 120
R / Rfree (%)	17 / 19.5

The structure of Hras R97F Crystal Form 1 also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Calcium	(Ca)	2 atoms

The binding sites of Magnesium atom in the Hras R97F Crystal Form 1 (pdb code 8elk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hras R97F Crystal Form 1, PDB code: 8elk:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hras R97F Crystal Form 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hras R97F Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:16.8 occ:1.00 O A:HOH334 2.0 17.5 1.0 OG A:SER17 2.0 15.2 1.0 O2B A:GNP201 2.0 15.7 1.0 O2G A:GNP201 2.0 18.2 1.0 OG1 A:THR35 2.1 17.5 1.0 O A:HOH351 2.2 18.4 1.0 CB A:SER17 3.1 18.3 1.0 CB A:THR35 3.1 19.6 1.0 PB A:GNP201 3.2 16.4 1.0 PG A:GNP201 3.3 18.1 1.0 N3B A:GNP201 3.5 16.3 1.0 N A:SER17 3.9 14.4 1.0 N A:THR35 3.9 17.8 1.0 OD2 A:ASP57 3.9 23.2 1.0 CA A:SER17 4.0 15.5 1.0 O3G A:GNP201 4.1 21.4 1.0 CA A:THR35 4.1 18.0 1.0 OD1 A:ASP57 4.1 20.8 1.0 CG2 A:THR35 4.1 18.5 1.0 O2A A:GNP201 4.2 18.5 1.0 O A:HOH374 4.2 24.6 1.0 O1B A:GNP201 4.3 16.6 1.0 O3A A:GNP201 4.3 14.2 1.0 O1G A:GNP201 4.4 18.2 1.0 CG A:ASP57 4.4 22.1 1.0 O A:ASP33 4.4 19.0 1.0 O A:THR58 4.5 21.9 1.0 PA A:GNP201 4.6 17.1 1.0 O1A A:GNP201 4.6 15.2 1.0 C A:PRO34 4.8 22.1 1.0 CB A:LYS16 4.9 15.5 1.0 C A:LYS16 4.9 17.7 1.0 CE A:LYS16 5.0 17.3 1.0

Magnesium binding site 2 out of 2 in the Hras R97F Crystal Form 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hras R97F Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg204 b:33.7 occ:0.33 O A:HOH327 2.1 33.5 1.0 O A:HOH340 2.2 34.1 1.0 O A:THR124 4.0 22.4 1.0 OE1 A:GLN129 4.1 26.4 0.5 NE2 A:GLN129 4.2 29.6 0.6 O A:HOH314 4.2 32.5 1.0 OE1 A:GLN129 4.4 26.2 0.6 CD A:GLN129 4.7 31.6 0.6 CD A:GLN129 4.7 28.4 0.5 NE2 A:GLN129 4.8 19.7 0.5

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767