Magnesium in PDB 8elt: Hras R97G Crystal Form 2

All present enzymatic activity of Hras R97G Crystal Form 2:
3.6.5.2;

The structure of Hras R97G Crystal Form 2, PDB code: 8elt was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.72 / 1.66
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	38.941, 38.941, 157.386, 90, 90, 120
R / Rfree (%)	19.8 / 23.9

The structure of Hras R97G Crystal Form 2 also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Magnesium atom in the Hras R97G Crystal Form 2 (pdb code 8elt). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hras R97G Crystal Form 2, PDB code: 8elt:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hras R97G Crystal Form 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hras R97G Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:22.6 occ:1.00 O2G A:GNP202 2.0 22.5 1.0 O2B A:GNP202 2.0 23.4 1.0 O A:HOH319 2.0 23.1 1.0 OG1 A:THR35 2.1 25.2 1.0 OG A:SER17 2.1 22.6 1.0 O A:HOH337 2.1 24.0 1.0 CB A:THR35 3.1 27.4 1.0 CB A:SER17 3.1 21.4 1.0 PB A:GNP202 3.2 22.4 1.0 PG A:GNP202 3.2 24.8 1.0 N3B A:GNP202 3.3 21.7 1.0 N A:THR35 3.7 25.6 1.0 N A:SER17 3.8 20.4 1.0 OD2 A:ASP57 4.0 26.3 1.0 CA A:THR35 4.0 25.8 1.0 CA A:SER17 4.0 21.1 1.0 OD1 A:ASP57 4.1 24.3 1.0 O2A A:GNP202 4.1 25.9 1.0 O3G A:GNP202 4.2 24.7 1.0 CG2 A:THR35 4.2 30.9 1.0 O1B A:GNP202 4.2 22.2 1.0 O A:HOH358 4.2 36.5 1.0 O3A A:GNP202 4.3 21.1 1.0 O1G A:GNP202 4.3 25.6 1.0 O A:THR58 4.4 27.9 1.0 CG A:ASP57 4.4 24.6 1.0 O A:ASP33 4.4 29.8 1.0 PA A:GNP202 4.5 22.8 1.0 O1A A:GNP202 4.6 22.7 1.0 C A:PRO34 4.7 29.4 1.0 CB A:LYS16 4.8 20.8 1.0 C A:LYS16 4.9 21.5 1.0 CE A:LYS16 4.9 22.5 1.0 CA A:PRO34 5.0 27.8 1.0

Magnesium binding site 2 out of 2 in the Hras R97G Crystal Form 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hras R97G Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:27.9 occ:1.00 O A:HOH351 2.3 43.1 1.0 O A:HOH315 2.3 42.0 1.0 OD1 A:ASP105 2.3 35.8 1.0 O A:ARG102 2.3 36.8 1.0 O A:HOH410 2.3 41.5 1.0 CG A:ASP105 3.3 32.8 1.0 C A:ARG102 3.5 35.6 1.0 OD2 A:ASP105 3.7 39.6 1.0 CA A:ARG102 4.2 32.1 1.0 N A:VAL103 4.5 29.4 1.0 CB A:ASP105 4.6 28.7 1.0 CA A:VAL103 4.6 32.0 1.0 O A:VAL103 4.7 35.2 1.0 N A:ASP105 4.7 28.3 1.0 CB A:ARG102 4.7 37.5 1.0 C A:VAL103 4.7 31.3 1.0 CA A:ASP105 4.8 29.3 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767