Magnesium in PDB 8elu: Hras R97G Crystal Form 1

All present enzymatic activity of Hras R97G Crystal Form 1:
3.6.5.2;

The structure of Hras R97G Crystal Form 1, PDB code: 8elu was solved by C.W.Johnson, J.A.Rodrigues, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.90 / 1.93
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.636, 88.636, 135.273, 90, 90, 120
R / Rfree (%)	17.7 / 20.9

The structure of Hras R97G Crystal Form 1 also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Magnesium atom in the Hras R97G Crystal Form 1 (pdb code 8elu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hras R97G Crystal Form 1, PDB code: 8elu:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hras R97G Crystal Form 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hras R97G Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:24.2 occ:1.00 O2G A:GNP202 2.0 21.5 1.0 O A:HOH322 2.0 19.1 1.0 OG1 A:THR35 2.0 19.9 1.0 OG A:SER17 2.1 21.0 1.0 O2B A:GNP202 2.1 21.2 1.0 O A:HOH348 2.1 24.3 1.0 CB A:THR35 3.0 24.7 1.0 PG A:GNP202 3.2 22.3 1.0 CB A:SER17 3.2 23.8 1.0 PB A:GNP202 3.3 22.8 1.0 N3B A:GNP202 3.4 23.2 1.0 N A:THR35 3.8 23.1 1.0 N A:SER17 3.9 19.7 1.0 OD2 A:ASP57 3.9 26.3 1.0 CA A:THR35 4.0 23.1 1.0 O3G A:GNP202 4.0 26.1 1.0 CG2 A:THR35 4.0 25.7 1.0 CA A:SER17 4.1 20.4 1.0 OD1 A:ASP57 4.1 25.1 1.0 O2A A:GNP202 4.2 21.5 1.0 O A:HOH356 4.2 29.8 1.0 O1B A:GNP202 4.2 20.1 1.0 O3A A:GNP202 4.3 20.8 1.0 O A:THR58 4.3 21.5 1.0 O1G A:GNP202 4.3 23.1 1.0 CG A:ASP57 4.4 23.7 1.0 O A:ASP33 4.5 23.3 1.0 PA A:GNP202 4.6 22.9 1.0 O1A A:GNP202 4.7 21.6 1.0 C A:PRO34 4.8 25.8 1.0 CB A:LYS16 4.8 21.1 1.0 CE A:LYS16 4.9 20.1 1.0 C A:LYS16 4.9 20.8 1.0 NZ A:LYS16 5.0 21.0 1.0

Magnesium binding site 2 out of 2 in the Hras R97G Crystal Form 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hras R97G Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:23.5 occ:0.33 O A:HOH382 2.1 25.5 1.0 O A:HOH325 2.2 23.6 1.0 O A:HOH442 3.8 53.4 0.5 NE2 A:GLN129 4.0 28.5 1.0 O A:THR124 4.1 21.5 1.0 O A:HOH330 4.2 22.2 1.0 OE1 A:GLN129 4.4 27.3 1.0 CD A:GLN129 4.6 34.0 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767