Magnesium in PDB 8elw: Hras R97A Crystal Form 1 T-State

All present enzymatic activity of Hras R97A Crystal Form 1 T-State:
3.6.5.2;

The structure of Hras R97A Crystal Form 1 T-State, PDB code: 8elw was solved by K.Davis, C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.65 / 1.70
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.226, 88.226, 133.839, 90, 90, 120
R / Rfree (%)	16 / 18.6

The structure of Hras R97A Crystal Form 1 T-State also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Magnesium atom in the Hras R97A Crystal Form 1 T-State (pdb code 8elw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hras R97A Crystal Form 1 T-State, PDB code: 8elw:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hras R97A Crystal Form 1 T-State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hras R97A Crystal Form 1 T-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:13.5 occ:1.00 O2G A:GNP604 2.0 14.9 1.0 O A:HOH719 2.1 13.6 1.0 OG A:SER17 2.1 12.1 1.0 O2B A:GNP604 2.1 12.1 1.0 O A:HOH730 2.1 14.3 1.0 OG1 A:THR35 2.1 13.1 1.0 CB A:THR35 3.1 14.8 1.0 CB A:SER17 3.1 13.5 1.0 PG A:GNP604 3.2 14.9 1.0 PB A:GNP604 3.2 13.3 1.0 N3B A:GNP604 3.4 14.2 1.0 N A:THR35 3.8 12.8 1.0 N A:SER17 3.9 10.9 1.0 OD2 A:ASP57 4.0 19.6 1.0 CA A:SER17 4.0 12.6 1.0 O3G A:GNP604 4.1 17.4 1.0 CA A:THR35 4.1 14.2 1.0 O A:HOH733 4.1 19.0 1.0 O2A A:GNP604 4.1 15.5 1.0 OD1 A:ASP57 4.1 17.8 1.0 CG2 A:THR35 4.2 14.8 1.0 O3A A:GNP604 4.2 10.5 1.0 O1B A:GNP604 4.3 13.8 1.0 O1G A:GNP604 4.4 16.6 1.0 O A:ASP33 4.4 14.5 1.0 CG A:ASP57 4.5 17.7 1.0 PA A:GNP604 4.5 12.8 1.0 O A:THR58 4.5 16.0 1.0 O1A A:GNP604 4.6 12.3 1.0 C A:PRO34 4.8 18.6 1.0 CB A:LYS16 4.8 12.6 1.0 C A:LYS16 4.9 12.3 1.0

Magnesium binding site 2 out of 2 in the Hras R97A Crystal Form 1 T-State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hras R97A Crystal Form 1 T-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:13.7 occ:0.37 O A:HOH771 2.1 16.0 1.0 O A:HOH725 2.1 14.2 1.0 O A:THR124 4.0 14.3 1.0 NE2 A:GLN129 4.0 24.2 1.0 O A:HOH720 4.2 21.2 1.0 OE1 A:GLN129 4.3 16.6 1.0 CD A:GLN129 4.6 26.5 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767