Magnesium in PDB 8fmg: Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)

The structure of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au), PDB code: 8fmg was solved by O.Rechkoblit, D.F.Kreitler, A.K.Aggarwal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.12 / 1.79
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	145.192, 80.522, 406.841, 90, 90.62, 90
R / Rfree (%)	17.9 / 22.5

The structure of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) also contains other interesting chemical elements:

Zinc

(Zn)

12 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 13;

Binding sites:

The binding sites of Magnesium atom in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) (pdb code 8fmg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 13 binding sites of Magnesium where determined in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au), PDB code: 8fmg:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:28.6 occ:1.00 NE2 A:HIS91 1.9 29.6 1.0 OD2 A:ASP95 2.1 27.0 1.0 O A:HOH582 2.1 31.5 1.0 O A:HOH632 2.1 32.0 1.0 O A:HOH666 2.1 28.4 1.0 O A:HOH529 2.1 27.2 1.0 CE1 A:HIS91 2.9 30.8 1.0 CD2 A:HIS91 2.9 29.2 1.0 CG A:ASP95 3.0 31.0 1.0 HE1 A:HIS91 3.1 37.0 1.0 HD2 A:HIS91 3.1 35.1 1.0 OD1 A:ASP95 3.2 32.1 1.0 H A:HIS56 3.6 33.7 1.0 HB1 A:ALA55 3.8 32.1 1.0 ND1 A:HIS91 4.0 27.0 1.0 HA A:ALA55 4.0 33.1 1.0 CG A:HIS91 4.0 27.2 1.0 O A:HOH588 4.1 31.0 1.0 O A:HOH704 4.2 31.2 1.0 O A:HOH569 4.3 30.9 1.0 N A:HIS56 4.3 28.1 1.0 O A:HIS56 4.3 33.1 1.0 CB A:ASP95 4.4 27.2 1.0 HB2 A:HIS56 4.5 44.9 1.0 CB A:ALA55 4.5 26.7 1.0 HB3 A:ASP95 4.6 32.6 1.0 HB2 A:ALA55 4.6 32.1 1.0 HB2 A:ASP95 4.6 32.6 1.0 CA A:ALA55 4.6 27.6 1.0 HD1 A:HIS91 4.8 32.4 1.0 HH21 A:ARG66 4.8 36.6 1.0 HD11 A:ILE94 4.9 40.7 1.0 HG2 A:PRO65 4.9 42.2 1.0 C A:ALA55 5.0 30.6 1.0

Magnesium binding site 2 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:44.1 occ:1.00 O A:HOH602 2.1 41.3 1.0 OE2 C:GLU53 2.5 36.0 1.0 OD2 A:ASP92 2.8 47.0 1.0 HB3 C:PRO88 2.8 36.5 1.0 OD1 A:ASP92 3.0 39.5 1.0 O A:HOH628 3.1 44.4 1.0 CG A:ASP92 3.2 42.9 1.0 O A:HOH537 3.2 34.4 1.0 HG3 C:PRO88 3.3 36.3 1.0 O C:HOH624 3.3 41.1 1.0 O A:HOH501 3.5 39.3 1.0 CD C:GLU53 3.6 32.8 1.0 CB C:PRO88 3.6 30.4 1.0 CG C:PRO88 3.8 30.3 1.0 HG2 C:PRO88 3.8 36.3 1.0 O C:HOH519 4.1 34.0 1.0 HB2 C:PRO88 4.2 36.5 1.0 HG3 C:GLU53 4.2 45.5 1.0 O A:HOH531 4.2 31.5 1.0 OE1 C:GLU53 4.3 31.8 1.0 HH21 A:ARG96 4.3 53.5 1.0 CG C:GLU53 4.4 37.9 1.0 HA C:PRO88 4.5 33.3 1.0 HB3 A:PRO88 4.5 34.0 1.0 CB A:ASP92 4.6 29.6 1.0 CA C:PRO88 4.7 27.7 1.0 HG2 C:GLU53 4.7 45.5 1.0 O A:PRO88 4.7 26.1 1.0 HB2 A:ASP92 4.8 35.6 1.0 MG C:MG403 4.8 44.9 1.0

Magnesium binding site 3 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:38.3 occ:1.00 NE2 C:HIS91 1.9 32.9 1.0 O C:HOH504 2.1 30.3 1.0 O C:HOH585 2.1 38.2 1.0 O C:HOH653 2.1 31.0 1.0 OD2 C:ASP95 2.1 32.8 1.0 O C:HOH659 2.1 35.0 1.0 CE1 C:HIS91 2.8 31.4 1.0 CD2 C:HIS91 2.9 33.0 1.0 HE1 C:HIS91 3.0 37.7 1.0 CG C:ASP95 3.0 30.1 1.0 HD2 C:HIS91 3.2 39.6 1.0 OD1 C:ASP95 3.2 33.3 1.0 H C:HIS56 3.5 39.6 1.0 HB1 C:ALA55 3.6 34.7 1.0 HA C:ALA55 4.0 34.4 1.0 ND1 C:HIS91 4.0 35.6 1.0 CG C:HIS91 4.1 25.4 1.0 O C:HOH542 4.2 39.7 1.0 N C:HIS56 4.2 33.0 1.0 O C:HIS56 4.3 30.5 1.0 O C:HOH620 4.3 35.8 1.0 CB C:ALA55 4.3 28.9 1.0 HB2 C:HIS56 4.4 40.6 1.0 HB2 C:ALA55 4.4 34.7 1.0 CB C:ASP95 4.4 31.2 1.0 CA C:ALA55 4.5 28.6 1.0 HB3 C:ASP95 4.6 37.5 1.0 HB2 C:ASP95 4.7 37.5 1.0 HD1 C:HIS91 4.7 42.7 1.0 O C:HOH546 4.7 36.1 1.0 HD11 C:ILE94 4.9 39.1 1.0 C C:ALA55 4.9 30.8 1.0 HH21 C:ARG66 5.0 44.1 1.0 HG2 C:PRO65 5.0 48.7 1.0

Magnesium binding site 4 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg403 b:44.9 occ:1.00 O C:HOH516 2.0 44.6 1.0 O A:HOH531 2.0 31.5 1.0 O C:HOH624 2.1 41.1 1.0 O C:HOH556 2.1 41.7 1.0 O C:HOH519 2.1 34.0 1.0 OD1 C:ASP92 2.2 40.0 1.0 CG C:ASP92 3.0 41.0 1.0 OD2 C:ASP92 3.3 42.6 1.0 O C:HOH620 4.1 35.8 1.0 O A:HOH637 4.2 41.1 1.0 OE1 C:GLU53 4.2 31.8 1.0 OE2 C:GLU53 4.3 36.0 1.0 HA C:ASP92 4.4 37.4 1.0 CB C:ASP92 4.4 33.0 1.0 OE2 A:GLU53 4.5 34.8 1.0 O C:PRO88 4.6 29.5 1.0 HB3 A:PRO88 4.6 34.0 1.0 HB3 C:PRO88 4.7 36.5 1.0 CD C:GLU53 4.7 32.8 1.0 H C:ASP92 4.7 33.5 1.0 HH21 C:ARG96 4.7 58.9 1.0 HB3 C:HIS91 4.8 33.2 1.0 CA C:ASP92 4.8 31.1 1.0 MG A:MG403 4.8 44.1 1.0 HB2 C:ASP92 4.8 39.7 1.0 N C:ASP92 4.9 27.9 1.0 HA C:PRO88 5.0 33.3 1.0

Magnesium binding site 5 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg402 b:23.9 occ:1.00 NE2 E:HIS91 1.9 28.8 1.0 OD2 E:ASP95 2.0 23.4 1.0 O E:HOH587 2.0 27.0 1.0 O E:HOH597 2.0 27.4 1.0 O E:HOH684 2.1 24.2 1.0 O E:HOH659 2.1 27.8 1.0 CE1 E:HIS91 2.9 30.8 1.0 CD2 E:HIS91 2.9 30.0 1.0 CG E:ASP95 2.9 27.0 1.0 HD2 E:HIS91 3.1 36.0 1.0 HE1 E:HIS91 3.1 36.9 1.0 OD1 E:ASP95 3.2 28.4 1.0 HB1 E:ALA55 3.6 35.2 1.0 H E:HIS56 3.6 35.7 1.0 O E:HOH577 3.9 33.2 1.0 ND1 E:HIS91 4.0 27.7 1.0 CG E:HIS91 4.0 25.7 1.0 HA E:ALA55 4.1 30.9 1.0 O E:HOH635 4.2 24.7 1.0 CB E:ASP95 4.3 24.6 1.0 CB E:ALA55 4.3 29.3 1.0 O E:HOH566 4.3 29.3 1.0 O E:HOH728 4.4 30.1 1.0 N E:HIS56 4.4 29.8 1.0 O E:HIS56 4.4 29.5 1.0 HB2 E:ALA55 4.4 35.2 1.0 HB2 E:ASP95 4.6 29.6 1.0 HB3 E:ASP95 4.6 29.6 1.0 HB2 E:HIS56 4.6 34.6 1.0 CA E:ALA55 4.7 25.7 1.0 HG2 E:PRO65 4.7 36.9 1.0 HD1 E:HIS91 4.8 33.2 1.0 HD11 E:ILE94 4.9 35.8 1.0 HH21 E:ARG66 4.9 32.6 1.0 HG13 E:ILE94 5.0 33.0 1.0 HG3 E:PRO65 5.0 36.9 1.0

Magnesium binding site 6 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg403 b:66.1 occ:1.00 O E:HOH519 2.1 35.1 1.0 O E:HOH656 2.1 51.5 1.0 O E:HOH735 2.1 41.8 1.0 O E:HOH642 2.1 34.1 1.0 O E:HOH542 2.1 40.2 1.0 OD1 E:ASP92 2.3 41.7 1.0 O E:HOH674 2.7 48.0 1.0 CG E:ASP92 3.1 37.6 1.0 OD2 E:ASP92 3.3 43.9 1.0 MG G:MG403 4.1 43.6 1.0 OE1 E:GLU53 4.2 30.3 1.0 O G:HOH618 4.3 43.2 1.0 O E:HOH577 4.3 33.2 1.0 OE2 E:GLU53 4.5 39.2 1.0 HB3 G:PRO88 4.6 32.7 1.0 CB E:ASP92 4.6 28.9 1.0 HA E:ASP92 4.6 36.8 1.0 O E:PRO88 4.7 26.1 1.0 HB3 E:PRO88 4.7 33.7 1.0 CD E:GLU53 4.7 33.2 1.0 OE2 G:GLU53 4.8 34.4 1.0 O G:HOH552 4.8 40.5 1.0 H E:ASP92 4.9 28.8 1.0 HB3 E:HIS91 4.9 28.8 1.0 HA E:PRO88 4.9 32.2 1.0

Magnesium binding site 7 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg402 b:27.9 occ:1.00 NE2 G:HIS91 1.9 22.8 1.0 O G:HOH658 2.1 29.2 1.0 O G:HOH581 2.1 19.4 1.0 O G:HOH655 2.1 25.6 1.0 O G:HOH666 2.1 26.8 1.0 OD2 G:ASP95 2.1 24.9 1.0 CE1 G:HIS91 2.9 23.9 1.0 CD2 G:HIS91 2.9 23.5 1.0 HE1 G:HIS91 3.0 28.7 1.0 CG G:ASP95 3.1 24.4 1.0 HD2 G:HIS91 3.1 28.2 1.0 OD1 G:ASP95 3.4 24.3 1.0 H G:HIS56 3.6 30.1 1.0 HB1 G:ALA55 3.9 29.7 1.0 ND1 G:HIS91 4.0 26.2 1.0 HA G:ALA55 4.0 28.4 1.0 CG G:HIS91 4.0 21.5 1.0 O G:HOH540 4.2 35.3 1.0 O G:HOH718 4.3 34.8 1.0 O G:HOH732 4.3 29.6 1.0 O G:HOH550 4.3 25.7 1.0 N G:HIS56 4.4 25.1 1.0 O G:HIS56 4.5 25.7 1.0 CB G:ASP95 4.5 27.7 1.0 CB G:ALA55 4.6 24.8 1.0 HB2 G:HIS56 4.6 38.0 1.0 HB2 G:ALA55 4.6 29.7 1.0 HB2 G:ASP95 4.7 33.2 1.0 CA G:ALA55 4.7 23.7 1.0 HB3 G:ASP95 4.7 33.2 1.0 HD11 G:ILE94 4.7 31.8 1.0 HD1 G:HIS91 4.7 31.4 1.0 HG2 G:PRO65 4.8 39.9 1.0 HH21 G:ARG66 4.9 31.0 1.0 O G:HIS91 5.0 22.5 1.0

Magnesium binding site 8 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg403 b:43.6 occ:1.00 O G:HOH524 2.3 38.0 1.0 OD2 G:ASP92 2.5 50.3 1.0 O E:HOH735 2.8 41.8 1.0 OE2 E:GLU53 2.9 39.2 1.0 HB3 E:PRO88 2.9 33.7 1.0 O G:HOH542 3.0 30.6 1.0 O E:HOH642 3.1 34.1 1.0 OD1 G:ASP92 3.2 38.4 1.0 CG G:ASP92 3.2 43.8 1.0 O G:HOH678 3.3 37.5 1.0 O G:HOH618 3.3 43.2 1.0 O E:HOH656 3.5 51.5 1.0 O E:HOH519 3.7 35.1 1.0 HG3 E:PRO88 3.8 34.1 1.0 CB E:PRO88 3.8 28.1 1.0 HB3 G:PRO88 3.9 32.7 1.0 CD E:GLU53 4.1 33.2 1.0 MG E:MG403 4.1 66.1 1.0 CG E:PRO88 4.2 28.4 1.0 HG2 E:PRO88 4.2 34.1 1.0 HB2 E:PRO88 4.3 33.7 1.0 O G:HOH505 4.4 33.9 1.0 O G:PRO88 4.5 24.1 1.0 OE1 E:GLU53 4.5 30.3 1.0 HA E:PRO88 4.6 32.2 1.0 HB2 G:PRO88 4.7 32.7 1.0 CB G:PRO88 4.7 27.2 1.0 CB G:ASP92 4.7 34.2 1.0 CA E:PRO88 4.8 26.8 1.0 HB2 G:ASP92 4.9 41.1 1.0 OE2 G:GLU53 4.9 34.4 1.0 O G:HOH646 5.0 44.6 1.0

Magnesium binding site 9 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ H:Mg402 b:33.5 occ:1.00 O H:ARG44 1.9 33.0 1.0 O H:HOH652 2.1 38.2 1.0 O H:HOH695 2.1 48.0 1.0 O H:HOH565 2.1 42.0 1.0 O H:HOH667 2.1 39.7 1.0 O H:HOH561 2.1 28.3 1.0 C H:ARG44 3.0 30.1 1.0 HA H:ARG44 3.2 36.6 1.0 CA H:ARG44 3.6 30.5 1.0 HB3 H:ARG44 3.9 39.1 1.0 HA H:ALA45 4.1 39.1 1.0 N H:ALA45 4.1 32.5 1.0 H H:GLY46 4.2 37.1 1.0 CB H:ARG44 4.3 32.6 1.0 O H:TYR43 4.4 28.7 1.0 CA H:ALA45 4.5 32.6 1.0 N H:GLY46 4.6 30.9 1.0 O G:HOH654 4.7 39.5 1.0 HB2 H:ARG44 4.8 39.1 1.0 N H:ARG44 4.8 29.2 1.0 C H:ALA45 4.9 29.2 1.0 H H:ALA45 4.9 39.0 1.0

Magnesium binding site 10 out of 13 in the Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Structure of Cbass CAP5 From Pseudomonas Syringae As An Activated Tetramer with the Cyclic Dinucleotide 3'2'-C-Diamp Ligand (3 Tetramers in the Au) within 5.0Å range: probe atom residue distance (Å) B Occ K:Mg402 b:28.3 occ:1.00 NE2 K:HIS91 1.9 23.5 1.0 OD2 K:ASP95 2.1 29.6 1.0 O K:HOH613 2.1 23.2 1.0 O K:HOH566 2.1 23.7 1.0 O K:HOH667 2.1 26.0 1.0 O K:HOH609 2.1 26.1 1.0 CD2 K:HIS91 2.9 25.6 1.0 CE1 K:HIS91 2.9 22.9 1.0 CG K:ASP95 3.0 26.0 1.0 HD2 K:HIS91 3.1 30.8 1.0 HE1 K:HIS91 3.1 27.5 1.0 OD1 K:ASP95 3.3 23.4 1.0 H K:HIS56 3.5 34.1 1.0 HB1 K:ALA55 3.6 34.6 1.0 ND1 K:HIS91 4.0 24.4 1.0 O K:HOH619 4.0 36.8 1.0 CG K:HIS91 4.0 23.6 1.0 HA K:ALA55 4.0 29.6 1.0 O K:HOH604 4.1 30.2 1.0 N K:HIS56 4.3 28.4 1.0 CB K:ALA55 4.3 28.9 1.0 O K:HIS56 4.4 24.1 1.0 CB K:ASP95 4.4 24.3 1.0 HB2 K:ALA55 4.4 34.6 1.0 O K:HOH730 4.4 35.1 1.0 O K:HOH582 4.5 26.3 1.0 O K:HOH714 4.5 44.8 1.0 HB2 K:HIS56 4.6 28.1 1.0 HB2 K:ASP95 4.6 29.2 1.0 CA K:ALA55 4.6 24.7 1.0 HB3 K:ASP95 4.7 29.2 1.0 HG2 K:PRO65 4.8 45.1 1.0 HD1 K:HIS91 4.8 29.3 1.0 HD2 K:HIS56 4.8 38.4 1.0 HH21 K:ARG66 4.9 30.0 1.0 C K:ALA55 5.0 24.9 1.0

O.Rechkoblit, D.Sciaky, D.F.Kreitler, A.Buku, J.Kottur, A.K.Aggarwal. Activation of Cbass-CAP5 Endonuclease Immune Effector By Cyclic Nucleotides: A View at High Resolution Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01220-X