Magnesium in PDB 8ghf: Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles

The structure of Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles also contains other interesting chemical elements:

Calcium	(Ca)	8 atoms
Sodium	(Na)	4 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles (pdb code 8ghf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles, PDB code: 8ghf:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1501 b:24.7 occ:1.00 OE2 A:GLU399 2.5 43.5 1.0 OE2 A:GLU374 3.1 42.7 1.0 CD A:GLU399 3.6 41.5 1.0 CD A:GLU374 4.1 37.4 1.0 CG A:GLU399 4.2 34.7 1.0 O A:THR396 4.5 37.0 1.0 OE1 A:GLU374 4.5 41.0 1.0 ND2 B:ASN172 4.5 59.0 1.0 OE1 A:GLU399 4.6 37.9 1.0 O A:PHE395 4.7 47.5 1.0 C A:THR396 4.8 38.1 1.0 OD1 B:ASN172 4.8 56.5 1.0 CA A:THR396 4.8 32.8 1.0

Magnesium binding site 2 out of 4 in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1501 b:25.3 occ:1.00 OE2 B:GLU399 2.5 43.5 1.0 OE2 B:GLU374 3.2 42.5 1.0 CD B:GLU399 3.6 41.0 1.0 CD B:GLU374 4.1 37.0 1.0 CG B:GLU399 4.2 34.9 1.0 OE1 B:GLU374 4.4 40.1 1.0 O B:THR396 4.5 37.8 1.0 ND2 D:ASN172 4.5 59.3 1.0 OE1 B:GLU399 4.6 38.0 1.0 O B:PHE395 4.7 47.1 1.0 C B:THR396 4.8 38.8 1.0 OD1 D:ASN172 4.8 57.3 1.0 CA B:THR396 4.8 33.6 1.0

Magnesium binding site 3 out of 4 in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1504 b:24.6 occ:1.00 OE2 C:GLU399 2.4 42.4 1.0 OE2 C:GLU374 3.1 42.1 1.0 CD C:GLU399 3.6 40.4 1.0 CD C:GLU374 4.1 37.0 1.0 CG C:GLU399 4.2 33.1 1.0 O C:THR396 4.5 36.9 1.0 OE1 C:GLU374 4.5 40.1 1.0 ND2 A:ASN172 4.5 57.4 1.0 OE1 C:GLU399 4.6 37.0 1.0 O C:PHE395 4.7 46.8 1.0 C C:THR396 4.8 37.6 1.0 OD1 A:ASN172 4.8 54.9 1.0 CA C:THR396 4.8 32.1 1.0

Magnesium binding site 4 out of 4 in the Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of HSLO1 in Plasma Membrane Vesicles within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1504 b:25.1 occ:1.00 OE2 D:GLU399 2.5 43.4 1.0 OE2 D:GLU374 3.2 44.0 1.0 CD D:GLU399 3.6 41.5 1.0 CD D:GLU374 4.1 38.3 1.0 CG D:GLU399 4.2 35.2 1.0 OE1 D:GLU374 4.4 41.8 1.0 O D:THR396 4.5 37.7 1.0 ND2 C:ASN172 4.5 59.5 1.0 OE1 D:GLU399 4.6 38.9 1.0 O D:PHE395 4.7 47.0 1.0 OD1 C:ASN172 4.8 57.3 1.0 C D:THR396 4.8 38.8 1.0 CA D:THR396 4.8 32.9 1.0

X.Tao, C.Zhao, R.Mackinnon. Membrane Protein Isolation and Structure Determination in Cell-Derived Membrane Vesicles. Proc.Natl.Acad.Sci.Usa V. 120 25120 2023.
ISSN: ESSN 1091-6490
PubMed: 37098056
DOI: 10.1073/PNAS.2302325120