Magnesium in PDB 8giz: E. Coli Clamp Loader with Open Clamp

All present enzymatic activity of E. Coli Clamp Loader with Open Clamp:
2.7.7.7;

The structure of E. Coli Clamp Loader with Open Clamp also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the E. Coli Clamp Loader with Open Clamp (pdb code 8giz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the E. Coli Clamp Loader with Open Clamp, PDB code: 8giz:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the E. Coli Clamp Loader with Open Clamp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of E. Coli Clamp Loader with Open Clamp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:74.9 occ:1.00 OG1 B:THR52 2.1 75.4 1.0 O2G B:AGS501 2.1 81.8 1.0 O2B B:AGS501 2.1 86.3 1.0 O3G B:AGS501 2.3 93.4 1.0 PG B:AGS501 2.6 77.3 1.0 HH22 C:ARG169 3.1 95.0 1.0 HB B:THR52 3.2 81.2 1.0 CB B:THR52 3.2 72.8 1.0 PB B:AGS501 3.3 61.8 1.0 O3B B:AGS501 3.3 77.7 1.0 HH22 B:ARG215 3.3 76.2 1.0 H B:THR52 3.6 83.0 1.0 O2A B:AGS501 3.7 86.6 1.0 NH2 C:ARG169 3.8 102.7 1.0 HG21 B:THR52 3.9 82.7 1.0 HH21 C:ARG169 3.9 94.6 1.0 NH2 B:ARG215 4.1 65.4 1.0 CG2 B:THR52 4.1 82.5 1.0 O1B B:AGS501 4.2 52.8 1.0 N B:THR52 4.2 86.2 1.0 CA B:THR52 4.3 70.8 1.0 HH21 B:ARG215 4.3 75.1 1.0 O3A B:AGS501 4.4 65.3 1.0 S1G B:AGS501 4.4 40.0 1.0 HB2 B:LYS51 4.4 75.4 1.0 OD2 B:ASP126 4.5 113.3 1.0 HZ1 B:LYS51 4.5 76.5 1.0 PA B:AGS501 4.6 67.7 1.0 HA B:THR52 4.6 79.8 1.0 HG23 B:THR52 4.7 81.4 1.0 HE2 B:LYS51 4.7 74.9 1.0 HZ2 B:LYS51 4.7 78.1 1.0 HH12 C:ARG169 4.7 94.9 1.0 HG22 B:THR52 4.8 81.6 1.0 HH12 B:ARG11 4.9 85.4 1.0 HH12 B:ARG215 4.9 76.7 1.0 CZ C:ARG169 5.0 101.0 1.0 NZ B:LYS51 5.0 84.8 1.0

Magnesium binding site 2 out of 3 in the E. Coli Clamp Loader with Open Clamp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of E. Coli Clamp Loader with Open Clamp within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:99.6 occ:1.00 OG1 C:THR52 2.0 78.2 1.0 O2G C:AGS501 2.0 87.0 1.0 O2B C:AGS501 2.4 85.3 1.0 PG C:AGS501 3.0 77.7 1.0 O3B C:AGS501 3.1 79.0 1.0 PB C:AGS501 3.3 69.1 1.0 CB C:THR52 3.3 74.2 1.0 H C:THR52 3.3 76.3 1.0 HB C:THR52 3.5 75.1 1.0 O3G C:AGS501 3.6 71.0 1.0 HB2 C:LYS51 3.8 81.3 1.0 HH21 D:ARG169 3.8 105.3 1.0 HH22 D:ARG169 3.9 104.7 1.0 N C:THR52 3.9 64.6 1.0 HE2 C:LYS51 4.0 82.1 1.0 O1B C:AGS501 4.0 72.6 1.0 HG21 C:THR52 4.2 76.3 1.0 CA C:THR52 4.2 62.3 1.0 NH2 D:ARG169 4.2 112.8 1.0 CG2 C:THR52 4.3 78.0 1.0 HH12 C:ARG215 4.4 85.0 1.0 HA C:THR52 4.4 74.0 1.0 O3A C:AGS501 4.5 88.3 1.0 HG23 C:THR52 4.7 75.3 1.0 S1G C:AGS501 4.7 86.0 1.0 CB C:LYS51 4.7 77.4 1.0 HZ1 C:LYS51 4.7 80.7 1.0 OD1 C:ASP126 4.7 116.3 1.0 O2A C:AGS501 4.9 84.5 1.0 CE C:LYS51 4.9 83.8 1.0 HB3 C:LYS51 4.9 80.7 1.0 C C:LYS51 5.0 75.0 1.0 HH21 C:ARG11 5.0 92.2 1.0

Magnesium binding site 3 out of 3 in the E. Coli Clamp Loader with Open Clamp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of E. Coli Clamp Loader with Open Clamp within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg502 b:62.7 occ:1.00 OG1 D:THR52 2.0 68.8 1.0 O2G D:AGS501 2.0 92.7 1.0 O2B D:AGS501 2.4 98.0 1.0 H D:THR52 3.1 78.6 1.0 CB D:THR52 3.2 74.6 1.0 HB D:THR52 3.2 77.0 1.0 PG D:AGS501 3.2 95.4 1.0 HH21 D:ARG215 3.3 86.1 1.0 O2A D:AGS501 3.4 85.8 1.0 OE2 E:GLU133 3.4 126.8 1.0 O3B D:AGS501 3.5 96.5 1.0 PB D:AGS501 3.5 91.8 1.0 HB2 D:LYS51 3.6 87.8 1.0 N D:THR52 3.8 79.9 1.0 O3G D:AGS501 3.9 83.6 1.0 NH2 D:ARG215 4.0 93.0 1.0 HH22 D:ARG215 4.0 85.8 1.0 CA D:THR52 4.1 66.9 1.0 HG21 D:THR52 4.1 77.7 1.0 CG2 D:THR52 4.3 79.3 1.0 CD E:GLU133 4.3 129.9 1.0 OE1 E:GLU133 4.4 129.2 1.0 HA D:THR52 4.4 74.4 1.0 HH21 E:ARG158 4.4 103.5 1.0 O1B D:AGS501 4.5 98.9 1.0 CB D:LYS51 4.5 78.0 1.0 O3A D:AGS501 4.6 84.9 1.0 PA D:AGS501 4.6 80.2 1.0 HH22 E:ARG158 4.6 102.4 1.0 NH2 E:ARG158 4.7 106.8 1.0 HG23 D:THR52 4.7 77.8 1.0 HB3 D:LYS51 4.7 88.4 1.0 S1G D:AGS501 4.8 103.3 1.0 C D:LYS51 4.9 89.6 1.0 HH22 D:ARG11 4.9 83.6 1.0 OD2 D:ASP126 5.0 112.0 1.0

Z.-Q.Xu, A.J.Oakley, N.E.Dixon. Structural Characterisation of the Complete Cycle of Sliding Clamp Loading in E. Coli To Be Published.