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Magnesium in PDB 8gvm: The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days

Enzymatic activity of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days

All present enzymatic activity of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days:
7.1.1.9;

Protein crystallography data

The structure of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days, PDB code: 8gvm was solved by T.Tsukihara, A.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.97 / 1.85
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 183.405, 206.272, 177.609, 90, 90, 90
R / Rfree (%) 16.4 / 18.8

Other elements in 8gvm:

The structure of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days also contains other interesting chemical elements:

Copper (Cu) 6 atoms
Zinc (Zn) 2 atoms
Iron (Fe) 6 atoms
Sodium (Na) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days (pdb code 8gvm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days, PDB code: 8gvm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 8gvm

Go back to Magnesium Binding Sites List in 8gvm
Magnesium binding site 1 out of 2 in the The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg604

b:25.3
occ:1.00
OD2 A:ASP369 2.1 26.9 1.0
OE1 B:GLU198 2.1 32.7 1.0
O B:HOH462 2.1 27.7 1.0
O B:HOH413 2.1 29.4 1.0
NE2 A:HIS368 2.1 24.4 1.0
O B:HOH499 2.1 27.6 1.0
CE1 A:HIS368 3.1 25.4 1.0
CD2 A:HIS368 3.1 28.8 1.0
CD B:GLU198 3.1 32.0 1.0
CG A:ASP369 3.2 27.7 1.0
OE2 B:GLU198 3.6 33.8 1.0
O B:SER197 3.9 26.2 1.0
CB A:ASP369 3.9 28.2 1.0
O A:HOH876 4.0 27.9 1.0
OD1 A:ASP369 4.2 28.7 1.0
ND1 A:HIS368 4.2 23.2 1.0
O A:HOH752 4.3 27.0 1.0
CG A:HIS368 4.3 25.5 1.0
OD2 B:ASP173 4.3 29.9 1.0
OD1 B:ASP173 4.3 29.3 1.0
OG1 A:THR294 4.4 27.8 1.0
CG B:GLU198 4.4 24.6 1.0
O A:HOH703 4.5 25.9 1.0
O A:HOH824 4.5 27.7 1.0
O B:HOH417 4.5 28.8 0.5
O A:HOH863 4.5 25.7 1.0
O B:HOH505 4.6 27.2 0.5
CB B:GLU198 4.6 29.0 1.0
CG B:ASP173 4.7 28.0 1.0
CA B:GLU198 4.9 31.9 1.0

Magnesium binding site 2 out of 2 in 8gvm

Go back to Magnesium Binding Sites List in 8gvm
Magnesium binding site 2 out of 2 in the The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Structure of Azide-Bound Cytochrome C Oxidase Determined Using the Crystals Exposed to 20 Mm Azide Solution For 4 Days within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Mg605

b:29.5
occ:1.00
OD2 N:ASP369 2.0 32.9 1.0
OE1 O:GLU198 2.0 39.4 1.0
O O:HOH454 2.1 33.7 1.0
O O:HOH415 2.1 33.5 1.0
O O:HOH489 2.1 33.0 1.0
NE2 N:HIS368 2.2 30.5 1.0
CE1 N:HIS368 3.1 30.1 1.0
CD2 N:HIS368 3.1 34.4 1.0
CD O:GLU198 3.2 38.4 1.0
CG N:ASP369 3.3 31.8 1.0
OE2 O:GLU198 3.7 40.3 1.0
O O:SER197 3.9 32.9 1.0
O N:HOH880 3.9 32.7 1.0
CB N:ASP369 4.0 38.3 1.0
OD1 N:ASP369 4.2 34.5 1.0
ND1 N:HIS368 4.2 29.7 1.0
O N:HOH768 4.2 31.3 1.0
CG N:HIS368 4.3 32.0 1.0
OD2 O:ASP173 4.3 36.3 1.0
O N:HOH709 4.4 33.7 1.0
OD1 O:ASP173 4.4 34.9 1.0
CG O:GLU198 4.4 31.4 1.0
OG1 N:THR294 4.4 34.2 1.0
O N:HOH827 4.5 31.8 1.0
O O:HOH439 4.6 30.2 0.5
O N:HOH853 4.6 31.6 1.0
CB O:GLU198 4.6 32.9 1.0
O O:HOH492 4.7 32.1 0.4
CG O:ASP173 4.7 33.3 1.0
CA O:GLU198 4.9 38.1 1.0
CA N:ASP369 5.0 35.4 1.0

Reference:

A.Shimada, K.Hatano, H.Tadehara, N.Yano, K.Sinzawa-Itoh, E.Yamashita, K.Mutamoto, T.Tsukihara, S.Yoshikawa. X-Ray Structural Analyses of Azide-Bound Cytochromecoxidases Reveal That the H-Pathway Is Critically Important For the Proton-Pumping Activity J. Biol. Chem. V. 293 14868 2018.
ISSN: ESSN ESSN 1083-351X
PubMed: 30077971
DOI: 10.1074/JBC.RA118.003123
Page generated: Fri Oct 4 03:59:04 2024

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