Magnesium in PDB 8i2r: Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose

All present enzymatic activity of Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose:
2.4.1.100;

The structure of Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose, PDB code: 8i2r was solved by T.Tonozuka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.82 / 1.36
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.542, 81.697, 99.602, 90, 90, 90
R / Rfree (%)	16.2 / 18.2

The binding sites of Magnesium atom in the Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose (pdb code 8i2r). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose, PDB code: 8i2r:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:17.1 occ:1.00 O A:HOH910 2.1 21.6 1.0 O A:HOH1216 2.1 27.6 1.0 O A:HOH837 2.1 17.2 1.0 O A:HOH919 2.3 22.8 1.0 O A:HOH1174 4.1 25.1 1.0 O A:HOH875 4.1 34.5 1.0 O A:ARG396 4.3 13.2 1.0 OD1 A:ASP404 4.3 15.9 1.0 O A:VAL403 4.4 17.7 1.0 O A:HOH1043 4.4 22.7 1.0 O A:TYR399 4.5 14.2 1.0 O A:HOH1217 4.5 34.6 1.0 CG A:ASP404 4.6 17.5 1.0 CB A:TYR399 4.7 11.8 1.0 OD2 A:ASP404 4.8 19.7 1.0

Magnesium binding site 2 out of 2 in the Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Beijerinckia Indica Beta-Fructosyltransferase Variant H395R/F473Y in Complex with Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:24.9 occ:1.00 O A:HOH1045 2.1 26.0 1.0 ND2 A:ASN451 2.3 21.5 1.0 O A:HOH1098 2.4 31.4 1.0 O A:HOH1148 2.6 36.9 1.0 O A:HOH914 2.9 42.5 1.0 O A:HOH791 3.3 45.9 1.0 CG A:ASN451 3.5 20.6 1.0 CA A:ASN451 4.1 19.2 1.0 CB A:ASN451 4.1 19.3 1.0 OD1 A:ASN451 4.4 24.4 1.0 O A:PRO450 4.9 23.4 1.0 O A:ASN451 4.9 21.6 1.0 N A:ASN451 5.0 18.3 1.0

D.Li, Y.Miyasaka, A.Kubota, T.Kozono, Y.Kitano, N.Sasaki, T.Fujii, T.Tochio, Y.Kadota, A.Nishikawa, T.Tonozuka. Characterization and Alteration of Product Specificity of Beijerinckia Indica Subsp. Indica Beta-Fructosyltransferase Biosci.Biotechnol.Biochem. 2023.
ISSN: ISSN 0916-8451
DOI: 10.1093/BBB/ZBAD074