Magnesium in PDB 8pk1: CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System

The binding sites of Magnesium atom in the CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System (pdb code 8pk1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System, PDB code: 8pk1:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:15.4 occ:1.00 OP1 H:DG13 2.3 1.1 1.0 OD1 A:ASP13 2.4 47.7 1.0 O A:PHE12 2.6 41.1 1.0 CB A:SER43 3.0 32.4 1.0 OG A:SER43 3.4 38.1 1.0 CG A:ASP13 3.6 44.3 1.0 C A:PHE12 3.7 40.3 1.0 P H:DG13 3.8 2.2 1.0 O D:PHE42 3.8 27.8 1.0 OE1 D:GLN41 4.2 46.4 1.0 OD2 A:ASP13 4.3 44.1 1.0 CA A:ASP13 4.4 42.3 1.0 N A:ASP13 4.4 42.8 1.0 CA A:SER43 4.5 25.9 1.0 O A:PHE42 4.5 30.9 1.0 N A:PHE12 4.5 35.5 1.0 O5' H:DG13 4.5 4.8 1.0 O3' H:DC12 4.5 2.7 1.0 O A:SER43 4.6 38.3 1.0 CB A:ASP13 4.6 39.8 1.0 CB D:PHE42 4.6 17.2 1.0 CB D:GLN41 4.7 29.0 1.0 OP2 H:DG13 4.7 3.6 1.0 CA A:PHE12 4.7 36.8 1.0 N D:PHE42 4.7 21.2 1.0 C A:SER43 4.8 30.9 1.0 C D:PHE42 4.8 20.6 1.0 CA D:PHE42 5.0 18.3 1.0

Magnesium binding site 2 out of 2 in the CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of CAS1-CAS2 Crispr Integrase Bound to Prespacer Dna, Streptococcus Thermophilus Dgcc 7710 CRISPR3 System within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg201 b:13.9 occ:1.00 OD1 D:ASP13 2.1 49.6 1.0 OP1 G:DT13 2.4 1.7 1.0 O D:PHE12 2.5 40.3 1.0 CB D:SER43 3.0 29.8 1.0 CG D:ASP13 3.4 45.3 1.0 OG D:SER43 3.4 33.8 1.0 C D:PHE12 3.5 39.6 1.0 O A:PHE42 3.8 30.9 1.0 P G:DT13 3.8 3.7 1.0 N D:ASP13 4.1 42.1 1.0 OD2 D:ASP13 4.2 43.6 1.0 CA D:ASP13 4.2 42.5 1.0 CB D:ASP13 4.3 38.4 1.0 OE1 A:GLN41 4.3 44.6 1.0 N D:PHE12 4.3 34.2 1.0 O5' G:DT13 4.4 5.2 1.0 CA D:SER43 4.5 22.1 1.0 O D:PHE42 4.5 27.8 1.0 CB A:PHE42 4.5 20.6 1.0 O D:SER43 4.5 36.8 1.0 CA D:PHE12 4.5 34.9 1.0 O3' G:DT12 4.6 2.2 1.0 N A:PHE42 4.7 25.4 1.0 CB A:GLN41 4.7 32.6 1.0 C D:SER43 4.8 27.9 1.0 C A:PHE42 4.8 24.7 1.0 OP2 G:DT13 4.8 4.8 1.0 CA A:PHE42 4.9 21.6 1.0

G.Sasnauskas, U.Gaizauskaite, G.Tamulaitiene. Structural Basis For Spacer Acquisition in A Type II-A Crispr-Cas System To Be Published.