Atomistry » Magnesium » PDB 8pmd-8qug » 8pou
Atomistry »
  Magnesium »
    PDB 8pmd-8qug »
      8pou »

Magnesium in PDB 8pou: Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution.

Enzymatic activity of Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution.

All present enzymatic activity of Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution.:
1.12.99.6;

Protein crystallography data

The structure of Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution., PDB code: 8pou was solved by A.Schmidt, J.Kalms, P.Scheerer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.92 / 1.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 73.231, 95.74, 120.005, 90, 90, 90
R / Rfree (%) 13.2 / 16.1

Other elements in 8pou:

The structure of Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution. also contains other interesting chemical elements:

Chlorine (Cl) 3 atoms
Iron (Fe) 13 atoms
Nickel (Ni) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution. (pdb code 8pou). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution., PDB code: 8pou:

Magnesium binding site 1 out of 1 in 8pou

Go back to Magnesium Binding Sites List in 8pou
Magnesium binding site 1 out of 1 in the Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the C19G/C120G Variant of the Membrane-Bound [Nife]-Hydrogenase From Cupriavidus Necator in the Air-Oxidized State at 1.65 A Resolution. within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Mg702

b:19.3
occ:1.00
 O L:HOH807 2.0 16.0 1.0
O L:HOH814 2.1 19.2 1.0
O L:CYS549 2.1 16.5 1.0
O L:HOH844 2.2 18.5 1.0
OE2 L:GLU56 2.2 19.3 1.0
NE2 L:HIS603 2.4 18.7 1.0
CD L:GLU56 3.2 20.2 1.0
CE1 L:HIS603 3.3 18.1 1.0
C L:CYS549 3.3 17.2 1.0
CD2 L:HIS603 3.4 16.7 1.0
OE1 L:GLU56 3.4 20.1 1.0
N L:CYS549 3.8 16.6 1.0
OE2 L:GLU347 4.0 20.3 1.0
OE1 L:GLN548 4.0 23.2 1.0
OE1 L:GLU347 4.0 19.9 1.0
CA L:CYS549 4.0 15.9 1.0
NZ L:LYS400 4.2 19.6 1.0
O L:HOH850 4.3 20.8 1.0
O L:HOH971 4.3 19.6 1.0
CB L:CYS549 4.4 17.0 1.0
N L:VAL550 4.4 15.7 1.0
CD L:LYS400 4.4 20.4 1.0
CD L:GLU347 4.4 19.8 1.0
ND1 L:HIS603 4.5 18.3 1.0
CG L:GLU56 4.5 19.8 1.0
CG L:HIS603 4.5 17.1 1.0
CE L:LYS400 4.6 20.3 1.0
CA L:VAL550 4.7 16.4 1.0
C L:GLN548 4.8 17.8 1.0
CG2 L:VAL550 5.0 19.4 1.0

Reference:

A.Schmidt, J.Kalms, C.Lorent, S.Katz, S.Frielingsdorf, R.M.Evans, J.Fritsch, E.Siebert, C.Teutloff, F.A.Armstrong, I.Zebger, O.Lenz, P.Scheerer. Stepwise Conversion of the Cys 6 [4FE-3S] to A Cys 4 [4FE-4S] Cluster and Its Impact on the Oxygen Tolerance of [Nife]-Hydrogenase. Chem Sci V. 14 11105 2023.
ISSN: ISSN 2041-6520
PubMed: 37860641
DOI: 10.1039/D3SC03739H
Page generated: Thu Dec 28 10:06:38 2023

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy