Magnesium in PDB 8rn8: Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))

All present enzymatic activity of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)):
2.7.7.48;

The binding sites of Magnesium atom in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) (pdb code 8rn8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)), PDB code: 8rn8:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg801 b:60.4 occ:1.00 OD2 A:ASP109 2.5 69.9 1.0 CG A:ASP109 3.2 68.7 1.0 OE2 A:GLU81 3.3 63.4 1.0 OD1 A:ASP109 3.5 71.1 1.0 OE2 A:GLU120 3.6 71.4 1.0 HG3 A:GLU81 3.8 62.6 1.0 HG2 A:GLU81 3.9 62.6 1.0 OE1 A:GLU120 4.0 73.3 1.0 HE2 A:HIS41 4.1 77.1 1.0 CD A:GLU81 4.1 62.7 1.0 CG A:GLU81 4.1 62.3 1.0 NE2 A:HIS41 4.2 77.6 1.0 CD A:GLU120 4.2 73.2 1.0 CB A:ASP109 4.3 65.7 1.0 HB3 A:ASP109 4.4 66.9 1.0 HA A:ASP109 4.4 66.0 1.0 O A:LEU107 4.5 47.8 1.0 CE1 A:HIS41 4.5 76.7 1.0 HE1 A:HIS41 4.6 76.7 1.0 CD2 A:HIS41 4.7 76.6 1.0 CA A:ASP109 4.8 64.8 1.0 N A:ASP109 4.9 67.9 1.0 HD2 A:HIS41 5.0 76.9 1.0

Magnesium binding site 2 out of 5 in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg801 b:33.2 occ:1.00 O B:GLU490 2.5 17.7 1.0 OD1 B:ASP445 2.7 30.1 1.0 HG23 B:THR303 2.8 21.6 1.0 HA B:PHE491 2.9 21.6 1.0 O B:PHE446 3.0 26.0 1.0 H B:GLY304 3.4 19.8 1.0 HG22 B:THR303 3.5 20.8 1.0 HD1 B:PHE491 3.5 22.6 1.0 C B:GLU490 3.5 21.0 1.0 CG2 B:THR303 3.5 22.7 1.0 CG B:ASP445 3.6 32.5 1.0 H B:PHE446 3.7 21.9 1.0 O B:GLY304 3.7 20.6 1.0 HA B:THR303 3.7 20.4 1.0 OD2 B:ASP445 3.8 29.9 1.0 C B:PHE446 3.8 12.2 1.0 CA B:PHE491 3.8 13.4 1.0 HB2 B:ALA447 3.9 20.8 1.0 H B:THR492 3.9 22.6 1.0 N B:GLY304 4.0 11.8 1.0 N B:PHE491 4.0 25.8 1.0 HG21 B:THR303 4.1 20.4 1.0 HB3 B:GLU490 4.1 21.5 1.0 N B:PHE446 4.2 26.3 1.0 CD1 B:PHE491 4.4 16.7 1.0 CA B:THR303 4.4 17.7 1.0 HA B:ALA447 4.4 20.6 1.0 OE2 B:GLU490 4.5 28.6 1.0 C B:THR303 4.5 19.4 1.0 N B:ALA447 4.5 9.9 1.0 H B:GLU490 4.5 21.7 1.0 CB B:THR303 4.5 15.4 1.0 HB2 B:PHE491 4.6 22.6 1.0 N B:THR492 4.6 23.5 1.0 CA B:PHE446 4.6 13.6 1.0 CD B:GLU490 4.6 28.1 1.0 CA B:GLU490 4.6 13.4 1.0 C B:GLY304 4.7 16.6 1.0 CB B:PHE491 4.7 18.3 1.0 HG2 B:GLU490 4.7 20.8 1.0 CB B:GLU490 4.7 15.8 1.0 C B:PHE491 4.7 17.9 1.0 CB B:ALA447 4.7 17.0 1.0 CA B:ALA447 4.8 11.6 1.0 HG1 B:THR303 4.8 20.8 1.0 H B:PHE491 4.9 23.3 1.0 HA B:PHE446 4.9 21.0 1.0 CA B:GLY304 4.9 13.1 1.0 CG B:GLU490 4.9 17.5 1.0 HD2 B:PHE489 5.0 21.1 1.0 OE1 B:GLU490 5.0 25.6 1.0

Magnesium binding site 3 out of 5 in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg802 b:33.3 occ:1.00 O B:SER442 2.9 32.9 1.0 HG B:SER442 3.0 26.0 1.0 OD1 B:ASP444 3.2 32.4 1.0 HB3 B:SER443 3.5 23.4 1.0 HB2 B:ASP445 3.7 21.8 1.0 HB3 B:ASP445 3.7 24.1 1.0 H B:ASP445 3.7 22.5 1.0 H B:ASP444 3.8 22.1 1.0 HG22 B:THR492 3.8 22.1 1.0 OG B:SER442 3.8 31.4 1.0 C B:SER442 4.2 24.6 1.0 HB3 B:SER442 4.2 25.2 1.0 CB B:ASP445 4.2 25.3 1.0 CG B:ASP444 4.3 23.3 1.0 CB B:SER443 4.4 24.1 1.0 N B:ASP445 4.4 26.4 1.0 HB2 B:SER443 4.5 24.4 1.0 CB B:SER442 4.5 27.6 1.0 N B:ASP444 4.5 23.4 1.0 HD13 B:LEU271 4.5 38.7 1.0 CG2 B:THR492 4.7 22.3 1.0 HG21 B:THR492 4.8 23.0 1.0 CA B:ASP445 4.9 15.1 1.0 OD2 B:ASP444 4.9 21.0 1.0

Magnesium binding site 4 out of 5 in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg801 b:48.7 occ:1.00 OD1 E:ASP444 2.1 68.3 1.0 HB2 E:ASP445 2.4 59.6 1.0 H E:ASP445 2.9 60.8 1.0 H E:ASP444 3.0 63.7 1.0 CB E:ASP445 3.1 59.7 1.0 HB3 E:ASP445 3.1 60.3 1.0 N E:ASP445 3.2 62.6 1.0 O E:SER442 3.2 66.8 1.0 HB2 E:SER443 3.3 64.0 1.0 CG E:ASP444 3.3 65.7 1.0 N E:ASP444 3.6 63.4 1.0 HG E:SER442 3.7 63.9 1.0 CA E:ASP445 3.7 60.6 1.0 C E:ASP444 3.8 63.1 1.0 CA E:ASP444 4.0 61.5 1.0 OD2 E:ASP444 4.1 65.2 1.0 CB E:SER443 4.2 63.6 1.0 HA E:ASP445 4.2 60.3 1.0 CB E:ASP444 4.2 63.2 1.0 OG E:SER442 4.3 66.7 1.0 CG E:ASP445 4.3 62.0 1.0 C E:SER442 4.4 62.7 1.0 C E:SER443 4.5 63.5 1.0 HB3 E:SER443 4.5 64.0 1.0 HB3 E:ASP444 4.6 63.5 1.0 O E:ASP444 4.7 63.7 1.0 OD2 E:ASP445 4.7 64.0 1.0 CA E:SER443 4.8 64.4 1.0 C E:ASP445 4.9 58.3 1.0 HA E:ASP444 5.0 63.3 1.0

Magnesium binding site 5 out of 5 in the Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S))


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Influenza B Polymerase Pseudo-Symmetrical Apo-Dimer (Flupol(E) |Flupol(S)) within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg802 b:55.1 occ:1.00 OD1 E:ASP445 2.3 60.3 1.0 O E:GLY304 2.8 53.6 1.0 CG E:ASP445 3.1 62.0 1.0 OD2 E:ASP445 3.2 64.0 1.0 HA E:PHE491 3.6 60.9 1.0 HG23 E:THR303 4.0 57.7 1.0 O E:GLU490 4.0 59.1 1.0 C E:GLY304 4.0 50.4 1.0 HB2 E:ASP305 4.0 56.1 1.0 HG22 E:THR303 4.1 57.6 1.0 H E:SER493 4.1 64.1 1.0 H E:PHE446 4.2 55.9 1.0 O E:PHE446 4.3 60.5 1.0 HG2 E:GLU490 4.4 59.2 1.0 H E:THR492 4.4 62.5 1.0 HB3 E:GLU490 4.4 59.5 1.0 H E:GLY304 4.5 52.2 1.0 CA E:PHE491 4.5 61.3 1.0 HA E:ASP305 4.5 56.4 1.0 CB E:ASP445 4.5 59.7 1.0 CG2 E:THR303 4.5 58.1 1.0 C E:GLU490 4.5 59.6 1.0 CD E:GLU490 4.5 60.0 1.0 HA E:ASP445 4.6 60.3 1.0 HD1 E:PHE491 4.6 60.4 1.0 OE2 E:GLU490 4.6 56.5 1.0 N E:GLY304 4.6 50.6 1.0 OE1 E:GLU490 4.7 59.8 1.0 N E:PHE491 4.8 61.0 1.0 N E:THR492 4.8 64.4 1.0 HB2 E:ASP445 4.8 59.6 1.0 N E:PHE446 4.8 53.9 1.0 CG E:GLU490 4.9 60.5 1.0 CB E:ASP305 4.9 55.4 1.0 CA E:GLY304 4.9 49.8 1.0 C E:PHE491 5.0 60.7 1.0 N E:ASP305 5.0 56.2 1.0 HG21 E:THR303 5.0 57.5 1.0 CA E:ASP305 5.0 56.1 1.0

B.Arragain, T.Krischuns, M.Pelosse, P.Drncova, M.Blackledge, N.Naffakh, S.Cusack. Structures of Influenza A and B Replication Complexes Give Insight Into Avian to Human Host Adaptation and Reveal A Role of ANP32 As An Electrostatic Chaperone For the Apo-Polymerase. Nat Commun V. 15 6910 2024.
ISSN: ESSN 2041-1723
PubMed: 39160148
DOI: 10.1038/S41467-024-51007-3