Magnesium in PDB 8sfo: Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site.

All present enzymatic activity of Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site.:
3.1.21.1; 4.6.1.22;

The binding sites of Magnesium atom in the Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site. (pdb code 8sfo). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site., PDB code: 8sfo:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1401 b:68.1 occ:1.00 OD1 A:ASP908 2.1 65.3 1.0 OE1 A:GLU993 2.5 62.4 1.0 OP1 D:DC31 2.9 82.9 1.0 CG A:ASP908 3.1 59.2 1.0 CD A:GLU993 3.2 61.4 1.0 OE2 A:GLU993 3.2 63.4 1.0 MG A:MG1402 3.3 73.7 1.0 O3' D:DC30 3.4 78.0 1.0 OD2 A:ASP908 3.6 60.3 1.0 P D:DC31 3.8 96.5 1.0 OG1 A:THR1070 3.9 58.6 1.0 CB A:ASP908 4.1 49.2 1.0 OG A:SER1071 4.2 73.2 1.0 O A:ARG909 4.4 58.8 1.0 C4' D:DC30 4.5 72.7 1.0 C3' D:DC30 4.5 74.4 1.0 CA A:ASP908 4.5 47.9 1.0 O5' D:DC31 4.5 88.7 1.0 CG A:GLU993 4.6 52.5 1.0 N A:ARG909 4.7 54.2 1.0 CA A:GLY1266 4.8 51.4 1.0 C5' D:DC30 4.9 73.5 1.0 CG1 A:ILE1270 4.9 42.4 1.0 C A:GLY1266 5.0 50.9 1.0 O A:GLY1266 5.0 58.7 1.0

Magnesium binding site 2 out of 2 in the Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1402 b:73.7 occ:1.00 OP1 D:DC31 2.1 82.9 1.0 OD2 A:ASP908 2.3 60.3 1.0 OD1 A:ASP1263 2.6 65.9 1.0 OD1 A:ASP908 2.9 65.3 1.0 CG A:ASP908 3.0 59.2 1.0 P D:DC31 3.3 96.5 1.0 MG A:MG1401 3.3 68.1 1.0 O5' D:DC31 3.4 88.7 1.0 CG A:ASP1263 3.5 67.7 1.0 OG A:SER1071 3.6 73.2 1.0 OD2 A:ASP1263 3.9 71.2 1.0 O A:ARG909 4.2 58.8 1.0 O3' D:DC30 4.2 78.0 1.0 OP2 D:DC31 4.4 86.1 1.0 CA A:ASP1263 4.4 53.4 1.0 CB A:ASP908 4.5 49.2 1.0 CB A:ASP1263 4.5 60.9 1.0 NH1 A:ARG1226 4.6 79.8 1.0 C5' D:DC31 4.7 80.2 1.0 CB A:SER1071 4.9 65.2 1.0 C A:ARG909 4.9 55.5 1.0

I.Strohkendl, D.W.Taylor. Wt Crispr-CAS12A with A 20BP R-Loop and Nontarget Strand in the Ruvc Active Site. To Be Published.