Magnesium in PDB 8tbg: Tricomplex of Rmc-7977, Hras Wt, and Cypa

All present enzymatic activity of Tricomplex of Rmc-7977, Hras Wt, and Cypa:
5.2.1.8;

The structure of Tricomplex of Rmc-7977, Hras Wt, and Cypa, PDB code: 8tbg was solved by A.Chen, A.C.A.Tomlinson, J.E.Knox, J.K.Yano, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.29 / 1.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	65.45, 80.86, 128.209, 90, 90, 90
R / Rfree (%)	16.1 / 19.2

The binding sites of Magnesium atom in the Tricomplex of Rmc-7977, Hras Wt, and Cypa (pdb code 8tbg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Tricomplex of Rmc-7977, Hras Wt, and Cypa, PDB code: 8tbg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Tricomplex of Rmc-7977, Hras Wt, and Cypa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tricomplex of Rmc-7977, Hras Wt, and Cypa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:11.7 occ:1.00 O1G A:GNP201 2.0 12.9 1.0 OG A:SER17 2.0 11.4 1.0 O2B A:GNP201 2.0 11.5 1.0 OG1 A:THR35 2.1 12.5 1.0 O A:HOH360 2.1 13.7 1.0 O A:HOH324 2.1 12.5 1.0 CB A:SER17 3.1 12.2 1.0 CB A:THR35 3.1 12.9 1.0 PG A:GNP201 3.2 12.9 1.0 PB A:GNP201 3.2 12.2 1.0 N3B A:GNP201 3.4 12.7 1.0 N A:THR35 3.8 13.0 1.0 N A:SER17 3.8 11.9 1.0 CA A:SER17 4.0 11.9 1.0 OD2 A:ASP57 4.1 13.3 1.0 CA A:THR35 4.1 12.6 1.0 O2G A:GNP201 4.1 14.2 1.0 O2A A:GNP201 4.1 14.3 1.0 O A:HOH376 4.2 27.2 1.0 OD1 A:ASP57 4.2 12.4 1.0 CG2 A:THR35 4.2 15.6 1.0 O1B A:GNP201 4.2 12.0 1.0 O3A A:GNP201 4.3 12.9 1.0 O3G A:GNP201 4.3 14.4 1.0 O A:ASP33 4.4 14.8 1.0 O A:THR58 4.4 14.2 1.0 CG A:ASP57 4.5 12.2 1.0 PA A:GNP201 4.5 12.7 1.0 O1A A:GNP201 4.6 12.7 1.0 C A:PRO34 4.7 14.2 1.0 CB A:LYS16 4.8 12.4 1.0 C A:LYS16 4.9 11.3 1.0 CA A:PRO34 4.9 14.5 1.0 CE A:LYS16 4.9 12.4 1.0

Magnesium binding site 2 out of 2 in the Tricomplex of Rmc-7977, Hras Wt, and Cypa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Tricomplex of Rmc-7977, Hras Wt, and Cypa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:9.8 occ:1.00 O1G B:GNP201 2.0 10.7 1.0 O2B B:GNP201 2.0 9.7 1.0 O B:HOH341 2.0 12.8 1.0 OG1 B:THR35 2.1 11.5 1.0 OG B:SER17 2.1 10.9 1.0 O B:HOH358 2.1 11.1 1.0 CB B:THR35 3.1 12.2 1.0 CB B:SER17 3.1 11.3 1.0 PB B:GNP201 3.2 9.8 1.0 PG B:GNP201 3.2 10.4 1.0 N3B B:GNP201 3.4 10.7 1.0 N B:THR35 3.7 10.7 1.0 N B:SER17 3.8 10.5 1.0 CA B:THR35 4.0 10.9 1.0 CA B:SER17 4.0 10.1 1.0 OD2 B:ASP57 4.1 14.1 1.0 O2G B:GNP201 4.1 11.6 1.0 O2A B:GNP201 4.1 11.0 1.0 CG2 B:THR35 4.2 12.4 1.0 OD1 B:ASP57 4.2 13.8 1.0 O B:HOH337 4.2 28.3 1.0 O1B B:GNP201 4.3 10.2 1.0 O3A B:GNP201 4.3 10.6 1.0 O3G B:GNP201 4.3 11.2 1.0 O B:ASP33 4.4 11.3 1.0 O B:THR58 4.5 12.0 1.0 CG B:ASP57 4.5 11.4 1.0 PA B:GNP201 4.5 10.3 1.0 O1A B:GNP201 4.7 11.4 1.0 C B:PRO34 4.7 12.5 1.0 CB B:LYS16 4.8 10.7 1.0 C B:LYS16 4.9 9.6 1.0 CA B:PRO34 4.9 11.6 1.0 CE B:LYS16 5.0 12.0 1.0

A.C.A.Tomlinson, A.Chen, J.E.Knox, J.K.Yano. Concurrent Inhibition of Oncogenic and Wild-Type Ras-Gtp For Cancer Therapy To Be Published.