Magnesium in PDB 8tbi: Tricomplex of Rmc-7977, Nras Wt, and Cypa

All present enzymatic activity of Tricomplex of Rmc-7977, Nras Wt, and Cypa:
3.6.5.2; 5.2.1.8;

The structure of Tricomplex of Rmc-7977, Nras Wt, and Cypa, PDB code: 8tbi was solved by A.C.A.Tomlinson, A.Chen, J.E.Knox, J.K.Yano, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.86 / 1.59
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	65.49, 84.27, 126.04, 90, 90, 90
R / Rfree (%)	18.1 / 20.8

The binding sites of Magnesium atom in the Tricomplex of Rmc-7977, Nras Wt, and Cypa (pdb code 8tbi). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Tricomplex of Rmc-7977, Nras Wt, and Cypa, PDB code: 8tbi:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Tricomplex of Rmc-7977, Nras Wt, and Cypa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tricomplex of Rmc-7977, Nras Wt, and Cypa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:25.7 occ:1.00 O2B A:GNP201 2.0 23.0 1.0 OG1 A:THR35 2.0 21.9 1.0 O A:HOH307 2.1 22.6 1.0 OG A:SER17 2.1 24.3 1.0 O A:HOH335 2.1 24.3 1.0 O1G A:GNP201 2.1 24.5 1.0 CB A:SER17 3.0 24.6 1.0 CB A:THR35 3.1 21.1 1.0 PB A:GNP201 3.2 25.1 1.0 PG A:GNP201 3.2 24.9 1.0 N3B A:GNP201 3.4 27.5 1.0 N A:THR35 3.8 24.7 1.0 N A:SER17 3.8 23.1 1.0 OD2 A:ASP57 4.0 27.1 1.0 CA A:SER17 4.0 23.6 1.0 OD1 A:ASP57 4.0 25.1 1.0 CA A:THR35 4.1 22.8 1.0 O2A A:GNP201 4.1 27.1 1.0 O A:HOH347 4.1 48.5 1.0 O2G A:GNP201 4.1 25.1 1.0 CG2 A:THR35 4.2 23.2 1.0 O1B A:GNP201 4.3 23.2 1.0 O3A A:GNP201 4.3 25.1 1.0 O3G A:GNP201 4.3 26.4 1.0 O A:ASP33 4.4 30.6 1.0 CG A:ASP57 4.4 23.9 1.0 O A:THR58 4.5 22.9 1.0 PA A:GNP201 4.5 26.0 1.0 O1A A:GNP201 4.6 27.9 1.0 C A:PRO34 4.8 26.6 1.0 CB A:LYS16 4.8 20.4 1.0 C A:LYS16 4.9 23.0 1.0 CE A:LYS16 4.9 23.2 1.0 CA A:PRO34 5.0 29.3 1.0

Magnesium binding site 2 out of 2 in the Tricomplex of Rmc-7977, Nras Wt, and Cypa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Tricomplex of Rmc-7977, Nras Wt, and Cypa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:18.9 occ:1.00 O1G B:GNP201 2.0 20.3 1.0 O2B B:GNP201 2.0 18.0 1.0 O B:HOH356 2.1 20.6 1.0 OG B:SER17 2.1 16.7 1.0 O B:HOH337 2.1 19.0 1.0 OG1 B:THR35 2.1 17.0 1.0 CB B:THR35 3.1 17.6 1.0 CB B:SER17 3.1 16.1 1.0 PB B:GNP201 3.2 20.2 1.0 PG B:GNP201 3.2 20.8 1.0 N3B B:GNP201 3.4 20.8 1.0 N B:THR35 3.7 18.4 1.0 N B:SER17 3.9 15.9 1.0 CA B:THR35 4.0 18.6 1.0 O2G B:GNP201 4.1 23.3 1.0 CA B:SER17 4.1 16.4 1.0 O2A B:GNP201 4.1 19.9 1.0 OD2 B:ASP57 4.1 22.0 1.0 O B:HOH320 4.2 46.5 1.0 OD1 B:ASP57 4.2 20.8 1.0 CG2 B:THR35 4.2 18.4 1.0 O1B B:GNP201 4.2 20.5 1.0 O3G B:GNP201 4.3 20.0 1.0 O3A B:GNP201 4.3 20.1 1.0 O B:ASP33 4.4 20.6 1.0 O B:THR58 4.4 20.5 1.0 CG B:ASP57 4.5 19.3 1.0 PA B:GNP201 4.5 20.5 1.0 O1A B:GNP201 4.6 21.1 1.0 C B:PRO34 4.7 20.6 1.0 CB B:LYS16 4.8 16.9 1.0 O B:HOH457 4.8 52.4 1.0 CE B:LYS16 4.8 18.1 1.0 CA B:PRO34 4.8 22.8 1.0 C B:LYS16 4.9 16.8 1.0

A.C.A.Tomlinson, A.Chen, J.E.Knox, J.K.Yano. Concurrent Inhibition of Oncogenic and Wild-Type Ras-Gtp For Cancer Therapy To Be Published.