Magnesium in PDB 8u1l: Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State

The binding sites of Magnesium atom in the Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State (pdb code 8u1l). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State, PDB code: 8u1l:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg802 b:227.6 occ:1.00 O3A A:ATP801 2.0 213.6 1.0 O1G A:ATP801 2.1 213.6 1.0 N A:GLY132 2.4 227.7 1.0 O3G A:ATP801 2.4 213.6 1.0 PG A:ATP801 2.5 213.6 1.0 CA A:GLY132 2.5 227.7 1.0 PA A:ATP801 2.6 213.6 1.0 OE1 A:GLU42 2.7 232.6 1.0 PB A:ATP801 2.7 213.6 1.0 O3B A:ATP801 2.8 213.6 1.0 O2B A:ATP801 2.9 213.6 1.0 O2A A:ATP801 3.0 213.6 1.0 C A:GLY132 3.5 227.7 1.0 CD A:GLU42 3.5 232.6 1.0 N A:PHE133 3.6 209.4 1.0 C A:VAL131 3.7 226.2 1.0 O1A A:ATP801 3.7 213.6 1.0 N A:VAL131 3.9 226.2 1.0 O5' A:ATP801 3.9 213.6 1.0 O2G A:ATP801 4.0 213.6 1.0 OE2 A:GLU42 4.0 232.6 1.0 O1B A:ATP801 4.0 213.6 1.0 OD1 A:ASN46 4.3 236.9 1.0 CA A:VAL131 4.4 226.2 1.0 CG A:GLU42 4.5 232.6 1.0 O A:GLY132 4.5 227.7 1.0 N A:GLY130 4.5 230.0 1.0 C A:GLY130 4.5 230.0 1.0 O A:VAL131 4.6 226.2 1.0 ND2 A:ASN46 4.6 236.9 1.0 CA A:GLY130 4.7 230.0 1.0 CG A:ASN46 4.9 236.9 1.0 CA A:PHE133 4.9 209.4 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of the RAF1-HSP90-CDC37 Complex in the Closed State within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg802 b:183.3 occ:1.00 O3G B:ATP801 1.9 169.4 1.0 PA B:ATP801 2.3 169.4 1.0 O2B B:ATP801 2.3 169.4 1.0 O2A B:ATP801 2.3 169.4 1.0 PG B:ATP801 2.4 169.4 1.0 O2G B:ATP801 2.5 169.4 1.0 O3A B:ATP801 2.5 169.4 1.0 PB B:ATP801 2.6 169.4 1.0 O3B B:ATP801 2.6 169.4 1.0 OE1 B:GLU42 2.7 162.2 1.0 N B:GLY132 2.9 181.1 1.0 CA B:GLY132 2.9 181.1 1.0 O1A B:ATP801 3.3 169.4 1.0 C B:GLY132 3.7 181.1 1.0 N B:PHE133 3.8 171.9 1.0 O5' B:ATP801 3.8 169.4 1.0 CD B:GLU42 3.8 162.2 1.0 O1G B:ATP801 3.9 169.4 1.0 O1B B:ATP801 4.0 169.4 1.0 C B:VAL131 4.2 170.2 1.0 OD1 B:ASN46 4.2 175.4 1.0 NH1 B:ARG391 4.3 160.8 1.0 N B:VAL131 4.3 170.2 1.0 OE2 B:GLU42 4.5 162.2 1.0 N B:GLY130 4.6 176.9 1.0 CA B:GLY127 4.6 172.2 1.0 C B:GLY130 4.7 176.9 1.0 O B:GLY132 4.7 181.1 1.0 CA B:GLY130 4.8 176.9 1.0 ND2 B:ASN46 4.8 175.4 1.0 CG B:GLU42 4.8 162.2 1.0 C5' B:ATP801 4.9 169.4 1.0 CA B:VAL131 4.9 170.2 1.0 CG B:ASN46 4.9 175.4 1.0 CA B:PHE133 5.0 171.9 1.0

L.I.Finci, M.Chakrabarti, G.Gulten, J.Finney, C.Grose, T.Fox, R.Yang, D.V.Nissley, F.Mccormick, D.Esposito, T.E.Balius, D.K.Simanshu. Structural Dynamics of RAF1-HSP90-CDC37 and HSP90 Complexes Reveal Asymmetric Client Interactions and Key Structural Elements. Commun Biol V. 7 260 2024.
ISSN: ESSN 2399-3642
PubMed: 38431713
DOI: 10.1038/S42003-024-05959-3