Atomistry » Magnesium » PDB 8uee-8un9 » 8umf
Atomistry »
  Magnesium »
    PDB 8uee-8un9 »
      8umf »

Magnesium in PDB 8umf: Structure of PSCAS9 in Complex with Grna and Dna in Product State

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of PSCAS9 in Complex with Grna and Dna in Product State (pdb code 8umf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 7 binding sites of Magnesium where determined in the Structure of PSCAS9 in Complex with Grna and Dna in Product State, PDB code: 8umf:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7;

Magnesium binding site 1 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 1 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1501

b:41.1
occ:1.00
OP2 C:DC43 2.1 42.3 1.0
OD1 A:ASP10 2.4 34.8 1.0
OE1 A:GLU690 2.6 43.5 1.0
CD A:GLU690 3.1 43.5 1.0
OE2 A:GLU690 3.3 43.5 1.0
CG A:ASP10 3.4 34.8 1.0
P C:DC43 3.6 42.3 1.0
OD2 A:ASP10 3.8 34.8 1.0
ND2 A:ASN692 4.0 41.3 1.0
CG A:GLU690 4.1 43.5 1.0
OD1 A:ASN692 4.1 41.3 1.0
O A:MET11 4.2 35.4 1.0
O5' C:DC43 4.2 42.3 1.0
C5' C:DC43 4.4 42.3 1.0
CB A:GLU690 4.5 43.5 1.0
CG A:ASN692 4.5 41.3 1.0
OP1 C:DC43 4.5 42.3 1.0
CB A:ASP10 4.6 34.8 1.0
CA A:ASP10 4.7 34.8 1.0
MG A:MG1502 4.8 34.8 1.0
N A:MET11 4.9 35.4 1.0
C A:ASP10 5.0 34.8 1.0

Magnesium binding site 2 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 2 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1502

b:34.8
occ:1.00
OD2 A:ASP10 2.1 34.8 1.0
OP1 C:DC43 2.1 42.3 1.0
ND1 A:HIS944 2.3 39.0 1.0
CE1 A:HIS944 3.1 39.0 1.0
P C:DC43 3.3 42.3 1.0
CG A:ASP10 3.3 34.8 1.0
CG A:HIS944 3.3 39.0 1.0
O A:TYR15 3.5 38.9 1.0
OP2 C:DC43 3.5 42.3 1.0
CB A:HIS944 3.7 39.0 1.0
OD1 A:ASP10 4.0 34.8 1.0
OP2 C:DT44 4.1 46.1 1.0
O A:MET11 4.2 35.4 1.0
OE1 A:GLN939 4.2 44.3 1.0
CA A:HIS944 4.2 39.0 1.0
O5' C:DC43 4.2 42.3 1.0
C5' C:DC43 4.2 42.3 1.0
NE2 A:HIS944 4.3 39.0 1.0
O A:ASP10 4.3 34.8 1.0
CB A:ASP10 4.4 34.8 1.0
CD2 A:HIS944 4.4 39.0 1.0
C A:TYR15 4.6 38.9 1.0
CA A:GLY12 4.7 35.8 1.0
OD2 A:ASP947 4.7 43.0 1.0
C A:MET11 4.8 35.4 1.0
C A:ASP10 4.8 34.8 1.0
MG A:MG1501 4.8 41.1 1.0
N A:GLY17 4.9 31.4 1.0

Magnesium binding site 3 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 3 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1503

b:34.7
occ:1.00
OD1 A:ASP756 2.1 33.4 1.0
OP2 D:DT34 2.2 31.4 1.0
OD1 A:ASN786 2.2 37.8 1.0
O3' E:DC35 2.3 34.0 1.0
CG A:ASP756 3.3 33.4 1.0
CG A:ASN786 3.4 37.8 1.0
P D:DT34 3.5 31.4 1.0
C3' E:DC35 3.6 34.0 1.0
O A:LYS789 3.7 42.4 1.0
O A:ASN786 3.8 37.8 1.0
O5' D:DT34 3.9 31.4 1.0
CB A:ASP756 4.1 33.4 1.0
ND2 A:ASN786 4.1 37.8 1.0
OD2 A:ASP756 4.2 33.4 1.0
C4' E:DC35 4.2 34.0 1.0
O A:HIS757 4.2 29.5 1.0
CD A:LYS789 4.4 42.4 1.0
CA A:ASP756 4.4 33.4 1.0
C5' E:DC35 4.4 34.0 1.0
NZ A:LYS789 4.4 42.4 1.0
N A:HIS757 4.5 29.5 1.0
CA A:ASN786 4.5 37.8 1.0
CG A:LYS789 4.5 42.4 1.0
CB A:ASN786 4.6 37.8 1.0
C A:ASN786 4.6 37.8 1.0
CB A:LYS789 4.7 42.4 1.0
C2' E:DC35 4.7 34.0 1.0
OP1 D:DT34 4.7 31.4 1.0
C A:LYS789 4.8 42.4 1.0
C A:ASP756 4.9 33.4 1.0

Magnesium binding site 4 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 4 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg201

b:15.6
occ:1.00
O4 B:U69 3.6 28.9 1.0
C4 B:U69 4.4 28.9 1.0
O6 B:G70 4.5 28.6 1.0
O4 B:U26 4.6 40.0 1.0
C5 B:U69 4.6 28.9 1.0

Magnesium binding site 5 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 5 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg202

b:20.3
occ:1.00
OP1 B:A114 3.7 29.3 1.0
OP2 B:A114 4.0 29.3 1.0
OP2 B:C97 4.1 27.7 1.0
O5' B:A114 4.1 29.3 1.0
P B:A114 4.1 29.3 1.0
OP2 B:U115 4.2 28.3 1.0
O6 B:G99 4.2 30.2 1.0
N7 B:G98 4.4 28.8 1.0
O6 B:G98 4.4 28.8 1.0
C5 B:C97 4.5 27.7 1.0
NH2 A:ARG279 4.5 22.2 1.0
N4 B:C116 4.8 27.5 1.0
C5' B:A114 4.8 29.3 1.0
N4 B:C97 4.9 27.7 1.0
C4 B:C97 4.9 27.7 1.0

Magnesium binding site 6 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 6 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg203

b:33.6
occ:1.00
O6 B:G105 3.6 31.9 1.0
N7 B:G105 4.2 31.9 1.0
O4 B:U104 4.2 27.3 1.0
C6 B:G105 4.4 31.9 1.0
C4 B:U104 4.5 27.3 1.0
C5 B:G105 4.7 31.9 1.0
N6 B:A106 4.7 37.0 1.0
C5 B:U104 4.9 27.3 1.0

Magnesium binding site 7 out of 7 in 8umf

Go back to Magnesium Binding Sites List in 8umf
Magnesium binding site 7 out of 7 in the Structure of PSCAS9 in Complex with Grna and Dna in Product State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Structure of PSCAS9 in Complex with Grna and Dna in Product State within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg204

b:23.1
occ:1.00
OP2 B:A94 3.7 32.6 1.0
OP2 B:C95 3.8 31.4 1.0
NH2 A:ARG329 4.0 25.7 1.0
OP2 B:C116 4.1 27.5 1.0
O5' B:A94 4.3 32.6 1.0
P B:A94 4.3 32.6 1.0
CZ A:ARG329 4.3 25.7 1.0
C4' B:U115 4.3 28.3 1.0
OP1 B:C116 4.4 27.5 1.0
NE A:ARG329 4.5 25.7 1.0
OP1 B:A94 4.5 32.6 1.0
C5 B:C95 4.5 31.4 1.0
P B:C116 4.5 27.5 1.0
O3' B:U115 4.5 28.3 1.0
C5' B:U115 4.8 28.3 1.0
C3' B:U115 5.0 28.3 1.0
NH1 A:ARG329 5.0 25.7 1.0

Reference:

J.P.K.Bravo, D.W.Taylor. Structure-Guided Engineering of PSCAS9 Yields A High Fidelity and Activity Enzyme For in Vivo Gene Editing To Be Published.
Page generated: Thu Oct 31 22:12:51 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy