Magnesium in PDB 8xom: Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate

All present enzymatic activity of Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate:
7.6.2.2; 7.6.2.3;

The binding sites of Magnesium atom in the Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate (pdb code 8xom). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate, PDB code: 8xom:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Human ABCC4 in Complex with Anp-Bound in NBD1 and Methotrexate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1418 b:265.7 occ:1.00 O2B A:ANP1401 2.0 270.2 1.0 N A:SER452 2.1 262.2 1.0 O2A A:ANP1401 2.1 262.2 1.0 PA A:ANP1401 2.4 266.0 1.0 O1A A:ANP1401 2.5 267.6 1.0 CA A:SER452 2.6 259.8 1.0 CB A:SER452 2.7 262.9 1.0 C A:LYS451 2.8 258.8 1.0 O3A A:ANP1401 2.9 268.8 1.0 PB A:ANP1401 3.0 275.3 1.0 OG A:SER452 3.1 262.4 1.0 CB A:LYS451 3.4 263.4 1.0 CA A:LYS451 3.4 261.9 1.0 N A:LYS451 3.6 265.9 1.0 OD2 A:ASP559 3.7 268.2 1.0 O A:LYS451 3.7 252.4 1.0 O1B A:ANP1401 3.8 272.8 1.0 O5' A:ANP1401 4.0 263.3 1.0 C A:SER452 4.0 256.1 1.0 N3B A:ANP1401 4.3 283.2 1.0 C A:GLY450 4.5 267.1 1.0 N A:SER453 4.6 257.7 1.0 C5' A:ANP1401 4.7 261.0 1.0 CG A:ASP559 4.8 270.2 1.0 CG A:LYS451 4.9 261.3 1.0 O A:SER452 4.9 252.6 1.0 O A:GLY450 5.0 264.8 1.0

Y.Zhu, X.Xing, F.Wang, L.Chen, C.Zhong, X.Lu, Z.Yu, Y.Yang, Y.Yao, Q.Song, S.Han, Z.Liu, P.Zhang. The Atp-Bound Inward-Open Conformation of ABCC4 Reveals Asymmetric Atp Binding For Substrate Transport. Febs Lett. 2024.
ISSN: ISSN 0014-5793
PubMed: 38886124
DOI: 10.1002/1873-3468.14955