Magnesium in PDB 8zyj: Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form

All present enzymatic activity of Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form:
7.2.2.13;

The structure of Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Fluorine	(F)	3 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form (pdb code 8zyj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form, PDB code: 8zyj:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Human Testis-Specific Na+,K+-Atpase ALPHA4 in Ouabain-Bound Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1101 b:36.7 occ:1.00 OD2 A:BFD384 1.9 32.3 1.0 F1 A:BFD384 1.9 48.0 1.0 O A:HOH1277 2.0 36.0 1.0 O A:THR386 2.0 33.0 1.0 O A:HOH1204 2.0 39.4 1.0 OD1 A:ASP723 2.2 35.0 1.0 CG A:BFD384 2.9 32.1 1.0 C A:THR386 3.2 33.2 1.0 CG A:ASP723 3.3 24.5 1.0 BE A:BFD384 3.3 24.2 1.0 OD1 A:BFD384 3.6 39.1 1.0 OD2 A:ASP723 3.7 38.7 1.0 OD2 A:ASP727 3.8 49.7 1.0 OG1 A:THR388 3.9 35.5 1.0 N A:GLY724 4.0 39.9 1.0 CA A:THR386 4.0 20.9 1.0 CB A:BFD384 4.1 23.2 1.0 N A:THR386 4.1 34.9 1.0 N A:GLY387 4.2 22.8 1.0 CB A:THR386 4.4 26.6 1.0 CA A:GLY387 4.4 15.0 1.0 N A:ASP723 4.4 36.5 1.0 F2 A:BFD384 4.4 42.0 1.0 O A:SER224 4.5 38.2 1.0 F3 A:BFD384 4.5 48.4 1.0 CB A:ASP723 4.6 33.5 1.0 CA A:GLY724 4.6 22.2 1.0 OD1 A:ASN726 4.6 36.7 1.0 N A:THR388 4.6 31.2 1.0 C A:GLY387 4.7 31.5 1.0 C A:LYS385 4.8 27.2 1.0 CA A:ASP723 4.8 30.0 1.0 C A:ASP723 4.8 33.6 1.0 CB A:THR388 4.9 26.8 1.0 CG A:ASP727 4.9 43.7 1.0

K.Abe, J.Mcdermott, H.V.Madapally, P.Marimuthu, C.C.Gopalasingam, C.Gerle, H.Shigematsu, H.Khandelia, G.Blanco. Molecular Structure of the Na+,K+-Atpase ALPHA4BETA1 Isoform in Its Ouabain-Bound Conformation. Int J Mol Sci V. 25 2024.
ISSN: ESSN 1422-0067
DOI: 10.3390/IJMS252212397