Magnesium in PDB 9duu: Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin

The binding sites of Magnesium atom in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin (pdb code 9duu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin, PDB code: 9duu:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:40.6 occ:1.00 O1B A:ADP401 2.1 53.2 1.0 OE1 A:GLN137 3.0 45.8 1.0 PB A:ADP401 3.2 50.3 1.0 O2B A:ADP401 3.5 37.8 1.0 CD A:GLN137 3.9 41.1 1.0 O3B A:ADP401 4.0 41.0 1.0 OD2 A:ASP154 4.0 45.9 1.0 O2A A:ADP401 4.1 44.6 1.0 CA A:GLY156 4.1 32.3 1.0 NE2 A:GLN137 4.2 42.9 1.0 O3A A:ADP401 4.5 44.3 1.0 OD1 A:ASP154 4.5 43.1 1.0 CG A:ASP154 4.7 45.4 1.0 CA A:GLY13 4.7 35.3 1.0 OD2 A:ASP11 4.8 48.5 1.0 N A:GLY156 4.9 22.5 1.0 PA A:ADP401 5.0 49.6 1.0

Magnesium binding site 2 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:29.3 occ:1.00 O1B B:ADP401 2.2 49.0 1.0 OE1 B:GLN137 2.9 53.6 1.0 PB B:ADP401 3.5 49.7 1.0 CD B:GLN137 3.8 45.0 1.0 O2B B:ADP401 3.9 40.7 1.0 O2A B:ADP401 4.0 49.4 1.0 OD2 B:ASP154 4.0 46.9 1.0 NE2 B:GLN137 4.1 41.5 1.0 CA B:GLY156 4.4 32.3 1.0 OD1 B:ASP154 4.4 40.8 1.0 O3B B:ADP401 4.4 40.0 1.0 OD2 B:ASP11 4.5 48.4 1.0 O3A B:ADP401 4.6 49.3 1.0 CG B:ASP154 4.6 43.9 1.0 OD1 B:ASP11 4.7 42.9 1.0 CG2 B:VAL339 4.8 31.3 1.0 CA B:GLY13 4.8 37.0 1.0 PA B:ADP401 4.9 56.0 1.0

Magnesium binding site 3 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:27.3 occ:1.00 O1B C:ADP401 2.4 46.7 1.0 OE1 C:GLN137 2.7 54.8 1.0 CD C:GLN137 3.6 48.8 1.0 OD2 C:ASP154 3.6 47.9 1.0 PB C:ADP401 3.7 38.4 1.0 NE2 C:GLN137 3.8 46.6 1.0 OD1 C:ASP154 4.0 44.0 1.0 O2B C:ADP401 4.0 37.7 1.0 O2A C:ADP401 4.2 43.0 1.0 CG C:ASP154 4.2 46.6 1.0 OD2 C:ASP11 4.4 44.2 1.0 CA C:GLY156 4.4 36.1 1.0 O3B C:ADP401 4.5 31.6 1.0 CG2 C:VAL339 4.6 31.7 1.0 OD1 C:ASP11 4.8 44.8 1.0 O3A C:ADP401 4.8 45.1 1.0 CG C:GLN137 4.9 34.7 1.0 CB C:VAL339 4.9 33.0 1.0

Magnesium binding site 4 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg402 b:35.8 occ:1.00 O1B D:ADP401 2.2 51.0 1.0 OE1 D:GLN137 2.8 52.6 1.0 PB D:ADP401 3.5 42.6 1.0 OD2 D:ASP154 3.6 45.2 1.0 CD D:GLN137 3.8 46.1 1.0 O2B D:ADP401 3.9 42.0 1.0 O2A D:ADP401 4.1 37.5 1.0 NE2 D:GLN137 4.1 45.8 1.0 OD1 D:ASP154 4.2 43.0 1.0 CA D:GLY156 4.3 37.7 1.0 O3B D:ADP401 4.3 33.3 1.0 CG D:ASP154 4.3 47.2 1.0 OD2 D:ASP11 4.5 44.0 1.0 O3A D:ADP401 4.7 38.4 1.0 CG2 D:VAL339 4.8 27.4 1.0 OD1 D:ASP11 4.9 48.6 1.0 N D:GLY156 5.0 27.2 1.0

Magnesium binding site 5 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg402 b:34.0 occ:1.00 O3B E:ADP401 2.3 54.6 1.0 OE1 E:GLN137 3.0 57.5 1.0 PB E:ADP401 3.3 48.0 1.0 O1B E:ADP401 3.3 45.8 1.0 O2A E:ADP401 3.6 45.6 1.0 CD E:GLN137 3.9 48.3 1.0 OD2 E:ASP154 4.1 46.0 1.0 NE2 E:GLN137 4.2 45.0 1.0 OD2 E:ASP11 4.2 43.0 1.0 CA E:GLY156 4.3 47.6 1.0 O2B E:ADP401 4.3 34.1 1.0 O3A E:ADP401 4.4 43.6 1.0 OD1 E:ASP154 4.5 46.8 1.0 PA E:ADP401 4.7 55.5 1.0 CA E:GLY13 4.7 37.3 1.0 OD1 E:ASP11 4.7 42.6 1.0 CG E:ASP154 4.8 48.0 1.0 CG2 E:VAL339 4.9 30.5 1.0 CG E:ASP11 4.9 40.5 1.0

Magnesium binding site 6 out of 6 in the Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Cryo-Em Structure of Recombinant Wildtype ACTA1 Phalloidin-Stabilized F-Actin within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg402 b:32.9 occ:1.00 O1B F:ADP401 2.3 50.4 1.0 OE1 F:GLN137 2.8 54.9 1.0 PB F:ADP401 3.6 44.9 1.0 OD2 F:ASP154 3.7 47.8 1.0 CD F:GLN137 3.8 44.8 1.0 O2A F:ADP401 4.0 42.5 1.0 O2B F:ADP401 4.0 39.0 1.0 OD1 F:ASP154 4.1 45.2 1.0 NE2 F:GLN137 4.2 38.0 1.0 CG F:ASP154 4.3 46.7 1.0 OD2 F:ASP11 4.4 44.4 1.0 O3B F:ADP401 4.5 34.9 1.0 CA F:GLY156 4.5 36.0 1.0 CG2 F:VAL339 4.5 30.7 1.0 O3A F:ADP401 4.7 42.3 1.0 OD1 F:ASP11 4.9 48.2 1.0 CB F:GLN137 4.9 38.0 1.0 CG F:GLN137 4.9 34.2 1.0 CB F:VAL339 5.0 26.9 1.0 PA F:ADP401 5.0 48.4 1.0

A.Garg, S.Jansen, L.Greenberg, R.Zhang, K.J.Lavine, M.J.Greenberg. Dilated Cardiomyopathy-Associated Skeletal Muscle Actin (ACTA1) Mutation R256H Disrupts Actin Structure and Function and Causes Cardiomyocyte Hypocontractility To Be Published.