Magnesium in PDB 9duv: Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin

The binding sites of Magnesium atom in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin (pdb code 9duv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin, PDB code: 9duv:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:65.4 occ:1.00 OE1 A:GLN137 3.1 76.4 1.0 OD2 A:ASP154 3.7 80.0 1.0 CD A:GLN137 3.8 71.7 1.0 NE2 A:GLN137 3.9 71.8 1.0 O2B A:ADP401 4.1 89.4 1.0 O1A A:ADP401 4.1 83.6 1.0 OD1 A:ASP154 4.2 75.8 1.0 O3B A:ADP401 4.2 70.7 1.0 CA A:GLY156 4.3 67.8 1.0 O3A A:ADP401 4.3 85.5 1.0 CG A:ASP154 4.4 75.9 1.0 PB A:ADP401 4.5 90.9 1.0 OD2 A:ASP11 4.5 81.0 1.0 CG2 A:VAL339 4.6 60.3 1.0 PA A:ADP401 4.8 97.9 1.0 CB A:VAL339 5.0 60.5 1.0

Magnesium binding site 2 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:67.8 occ:1.00 NE2 B:GLN137 3.2 78.6 1.0 O2B B:ADP401 3.3 93.6 1.0 OE1 B:GLN137 3.7 76.3 1.0 CD B:GLN137 3.8 78.1 1.0 O3B B:ADP401 3.9 65.3 1.0 PB B:ADP401 4.0 91.1 1.0 O1A B:ADP401 4.0 87.6 1.0 OD2 B:ASP154 4.0 83.6 1.0 O3A B:ADP401 4.2 89.2 1.0 OD2 B:ASP11 4.4 85.6 1.0 CA B:GLY156 4.4 75.5 1.0 OD1 B:ASP154 4.5 76.9 1.0 OD1 B:ASP11 4.6 83.2 1.0 CA B:GLY13 4.7 82.3 1.0 CG B:ASP154 4.7 78.5 1.0 PA B:ADP401 4.7 100.6 1.0 CG2 B:VAL339 4.8 65.8 1.0 CG B:ASP11 5.0 83.5 1.0

Magnesium binding site 3 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:64.2 occ:1.00 OE1 C:GLN137 3.0 87.8 1.0 O2B C:ADP401 3.5 93.4 1.0 CD C:GLN137 3.7 81.8 1.0 OD2 C:ASP154 3.7 83.2 1.0 NE2 C:GLN137 3.8 81.2 1.0 O3B C:ADP401 4.1 68.7 1.0 OD1 C:ASP154 4.2 77.0 1.0 PB C:ADP401 4.2 90.2 1.0 O1A C:ADP401 4.2 93.1 1.0 OD2 C:ASP11 4.3 79.2 1.0 O3A C:ADP401 4.3 82.5 1.0 CA C:GLY156 4.4 80.0 1.0 CG C:ASP154 4.4 79.5 1.0 CG2 C:VAL339 4.5 65.0 1.0 OD1 C:ASP11 4.8 78.6 1.0 CB C:VAL339 4.8 63.3 1.0 PA C:ADP401 4.9 102.1 1.0 CG C:GLN137 4.9 66.3 1.0 CG C:ASP11 5.0 79.8 1.0

Magnesium binding site 4 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg402 b:67.0 occ:1.00 OE1 D:GLN137 3.0 87.1 1.0 OD2 D:ASP154 3.5 82.5 1.0 CD D:GLN137 3.7 81.4 1.0 NE2 D:GLN137 3.8 79.1 1.0 O2B D:ADP401 3.8 93.2 1.0 OD1 D:ASP154 4.0 77.3 1.0 O3B D:ADP401 4.2 64.5 1.0 CA D:GLY156 4.2 70.0 1.0 CG D:ASP154 4.2 81.3 1.0 O1A D:ADP401 4.3 86.3 1.0 O3A D:ADP401 4.4 82.6 1.0 PB D:ADP401 4.4 88.8 1.0 CG2 D:VAL339 4.5 61.9 1.0 OD2 D:ASP11 4.6 76.3 1.0 PA D:ADP401 4.9 92.3 1.0 N D:GLY156 4.9 65.5 1.0 CG D:GLN137 4.9 68.5 1.0 CB D:VAL339 5.0 63.6 1.0

Magnesium binding site 5 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg402 b:62.9 occ:1.00 O2B E:ADP401 2.3 96.0 1.0 OE1 E:GLN137 3.3 91.3 1.0 PB E:ADP401 3.7 87.7 1.0 NE2 E:GLN137 3.7 83.3 1.0 CD E:GLN137 3.8 86.2 1.0 OD2 E:ASP154 3.9 86.2 1.0 O1A E:ADP401 3.9 86.7 1.0 O3A E:ADP401 4.1 75.1 1.0 OD2 E:ASP11 4.3 79.1 1.0 OD1 E:ASP154 4.3 81.9 1.0 CA E:GLY156 4.4 80.7 1.0 O3B E:ADP401 4.5 66.0 1.0 CG E:ASP154 4.6 81.3 1.0 CG2 E:VAL339 4.6 61.5 1.0 PA E:ADP401 4.6 83.8 1.0 O1B E:ADP401 4.7 87.3 1.0 OD1 E:ASP11 4.9 84.9 1.0 CA E:GLY13 4.9 76.3 1.0 CB E:VAL339 5.0 61.2 1.0

Magnesium binding site 6 out of 6 in the Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Cryo-Em Structure of Recombinant R254H ACTA1 Phalloidin-Stabilized F- Actin within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg402 b:57.9 occ:1.00 OE1 F:GLN137 3.2 89.0 1.0 CD F:GLN137 3.8 78.2 1.0 OD2 F:ASP154 3.9 77.2 1.0 NE2 F:GLN137 3.9 74.6 1.0 O2B F:ADP401 3.9 93.4 1.0 CA F:GLY156 4.3 74.8 1.0 OD1 F:ASP154 4.4 79.0 1.0 OD2 F:ASP11 4.4 80.6 1.0 CG2 F:VAL339 4.6 66.6 1.0 CG F:ASP154 4.6 74.5 1.0 O1B F:ADP401 4.6 82.8 1.0 PB F:ADP401 4.9 83.6 1.0 OD1 F:ASP11 4.9 79.5 1.0 CB F:VAL339 4.9 60.3 1.0

A.Garg, S.Jansen, L.Greenberg, R.Zhang, K.J.Lavine, M.J.Greenberg. Dilated Cardiomyopathy-Associated Skeletal Muscle Actin (ACTA1) Mutation R256H Disrupts Actin Structure and Function and Causes Cardiomyocyte Hypocontractility To Be Published.