Magnesium in PDB 9e8n: NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase

All present enzymatic activity of NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase:
3.4.25.1;

The structure of NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase (pdb code 9e8n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase, PDB code: 9e8n:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:50.0 occ:1.00 O3G E:ATP501 1.9 20.0 1.0 OD2 E:ASP233 1.9 91.4 1.0 OG1 E:THR181 2.0 87.2 1.0 OD1 E:ASP233 2.1 91.4 1.0 CG E:ASP233 2.4 91.4 1.0 CB E:THR181 3.3 87.2 1.0 PG E:ATP501 3.3 20.0 1.0 CG2 E:THR181 3.6 87.2 1.0 O2B E:ATP501 3.6 20.0 1.0 O2G E:ATP501 3.8 20.0 1.0 CB E:ASP233 3.9 91.4 1.0 O1G E:ATP501 4.1 20.0 1.0 CA E:THR181 4.2 87.2 1.0 O3B E:ATP501 4.3 20.0 1.0 O1A E:ATP501 4.4 20.0 1.0 N E:THR181 4.4 87.2 1.0 ND2 F:ASN315 4.4 104.7 1.0 PB E:ATP501 4.5 20.0 1.0 NH1 F:ARG347 4.6 88.1 1.0 CA E:ASP233 4.9 91.4 1.0 OD1 F:ASN315 4.9 104.7 1.0 CG E:GLU234 5.0 89.8 1.0 N E:GLU234 5.0 89.8 1.0

Magnesium binding site 2 out of 2 in the NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of NUB1/FAT10-Processing Human 26S Proteasome Bound to TXNL1 with RPT3 at Top of Spiral Staircase within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg502 b:66.8 occ:1.00 O3G F:ATP501 2.1 51.2 1.0 OG1 F:THR234 2.2 69.6 1.0 O2G F:ATP501 2.9 51.2 1.0 OD2 F:ASP286 2.9 80.0 1.0 PG F:ATP501 3.0 51.2 1.0 OD1 F:ASP286 3.0 80.0 1.0 CG F:ASP286 3.3 80.0 1.0 O2B F:ATP501 3.5 51.2 1.0 CB F:THR234 3.6 69.6 1.0 NH1 A:ARG336 3.7 58.6 1.0 O1G F:ATP501 3.9 51.2 1.0 O3B F:ATP501 4.1 51.2 1.0 O2A F:ATP501 4.2 51.2 1.0 CG2 F:THR234 4.3 69.6 1.0 PB F:ATP501 4.4 51.2 1.0 N F:THR234 4.4 69.6 1.0 CA F:THR234 4.5 69.6 1.0 O A:ASP307 4.7 67.0 1.0 NH1 A:ARG333 4.7 59.6 1.0 NZ F:LYS233 4.8 64.3 1.0 CB F:ASP286 4.8 80.0 1.0 CZ A:ARG336 4.9 58.6 1.0 ND2 F:ASN333 4.9 63.9 1.0

C.Arkinson, C.L.Gee, Z.Zhang, K.C.Dong, A.Martin. Structural Landscape of Aaa+ Atpase Motor States in the Substrate-Degrading Human 26S Proteasome Reveals Conformation-Specific Binding of TXNL1. Biorxiv 2024.
ISSN: ISSN 2692-8205
PubMed: 39574680
DOI: 10.1101/2024.11.08.622731