Magnesium in PDB 6pen: Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide

All present enzymatic activity of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide:
5.6.1.1;

The structure of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide also contains other interesting chemical elements:

Fluorine

(F)

12 atoms

The binding sites of Magnesium atom in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide (pdb code 6pen). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide, PDB code: 6pen:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg703 b:84.4 occ:1.00 O2B A:ADP701 2.1 84.4 1.0 OG1 A:THR389 2.2 84.7 1.0 F1 A:BEF702 2.2 84.4 1.0 O1B A:ADP701 2.7 84.4 1.0 PB A:ADP701 2.8 84.4 1.0 OD2 A:ASP441 2.9 86.2 1.0 BE A:BEF702 3.3 84.4 1.0 O3B A:ADP701 3.4 84.4 1.0 CB A:THR389 3.4 84.7 1.0 CG A:ASP441 3.7 86.2 1.0 OD1 A:ASP441 3.7 86.2 1.0 NH2 B:ARG499 4.0 73.7 1.0 N A:THR389 4.2 84.7 1.0 O3A A:ADP701 4.2 84.4 1.0 F3 A:BEF702 4.3 84.4 1.0 CA A:THR389 4.4 84.7 1.0 NH2 B:ARG498 4.4 76.5 1.0 F2 A:BEF702 4.4 84.4 1.0 CG2 A:THR389 4.4 84.7 1.0 OD1 B:ASP470 4.4 80.3 1.0 O1A A:ADP701 4.8 84.4 1.0 CB A:LYS388 5.0 81.1 1.0 OD2 B:ASP470 5.0 80.3 1.0

Magnesium binding site 2 out of 4 in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg703 b:63.5 occ:1.00 O2B B:ADP701 2.1 63.5 1.0 OG1 B:THR389 2.1 64.2 1.0 F1 B:BEF702 2.2 63.5 1.0 PB B:ADP701 2.9 63.5 1.0 O1B B:ADP701 2.9 63.5 1.0 OD2 B:ASP441 3.0 69.0 1.0 BE B:BEF702 3.4 63.5 1.0 CB B:THR389 3.5 64.2 1.0 O3B B:ADP701 3.5 63.5 1.0 OD1 B:ASP441 3.6 69.0 1.0 CG B:ASP441 3.7 69.0 1.0 N B:THR389 4.0 64.2 1.0 CA B:THR389 4.2 64.2 1.0 F3 B:BEF702 4.3 63.5 1.0 O3A B:ADP701 4.4 63.5 1.0 F2 B:BEF702 4.4 63.5 1.0 CG2 B:THR389 4.5 64.2 1.0 OD2 C:ASP470 4.8 65.2 1.0 NH2 C:ARG498 4.8 64.0 1.0 NH2 C:ARG499 4.9 61.6 1.0 CB B:LYS388 4.9 63.5 1.0 OD1 C:ASP470 4.9 65.2 1.0

Magnesium binding site 3 out of 4 in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg703 b:57.4 occ:1.00 O2B C:ADP701 2.1 57.4 1.0 OG1 C:THR389 2.2 54.6 1.0 F1 C:BEF702 2.2 57.4 1.0 PB C:ADP701 2.9 57.4 1.0 O1B C:ADP701 2.9 57.4 1.0 OD2 C:ASP441 3.0 60.9 1.0 OD1 C:ASP441 3.2 60.9 1.0 BE C:BEF702 3.3 57.4 1.0 O3B C:ADP701 3.4 57.4 1.0 CB C:THR389 3.5 54.6 1.0 CG C:ASP441 3.5 60.9 1.0 N C:THR389 3.9 54.6 1.0 CA C:THR389 4.1 54.6 1.0 F3 C:BEF702 4.3 57.4 1.0 O3A C:ADP701 4.4 57.4 1.0 F2 C:BEF702 4.4 57.4 1.0 CG2 C:THR389 4.5 54.6 1.0 NH2 D:ARG499 4.6 60.6 1.0 CB C:LYS388 4.6 53.6 1.0 C C:LYS388 4.9 53.6 1.0 NH2 D:ARG498 4.9 60.5 1.0 CB C:ALA485 4.9 55.7 1.0 CB C:ASP441 5.0 60.9 1.0

Magnesium binding site 4 out of 4 in the Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of Spastin Hexamer (Whole Model) in Complex with Substrate Peptide within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg703 b:78.4 occ:1.00 OG1 D:THR389 1.9 80.3 1.0 O2B D:ADP701 2.0 78.4 1.0 F1 D:BEF702 2.0 78.4 1.0 O1B D:ADP701 2.4 78.4 1.0 PB D:ADP701 2.7 78.4 1.0 BE D:BEF702 3.2 78.4 1.0 CB D:THR389 3.2 80.3 1.0 O3B D:ADP701 3.4 78.4 1.0 OD2 D:ASP441 3.7 75.2 1.0 F3 D:BEF702 3.8 78.4 1.0 N D:THR389 3.8 80.3 1.0 CA D:THR389 4.1 80.3 1.0 OD1 D:ASP441 4.1 75.2 1.0 O3A D:ADP701 4.1 78.4 1.0 CG2 D:THR389 4.2 80.3 1.0 CG D:ASP441 4.3 75.2 1.0 F2 D:BEF702 4.5 78.4 1.0 NH2 E:ARG499 4.6 98.5 1.0 O1A D:ADP701 4.6 78.4 1.0 O2A D:ADP701 4.6 78.4 1.0 PA D:ADP701 4.7 78.4 1.0 CB D:LYS388 4.8 73.6 1.0 C D:LYS388 4.9 73.6 1.0

H.Han, H.L.Schubert, J.Mccullough, N.Monroe, W.I.Sundquist, C.P.Hill. Structure of the Microtubule Severing Protein Spastin Bound to A Glutamte-Rich Peptide Implies A Hand-Over-Hand Mechanism of Substrate Translocation. To Be Published.