Magnesium in PDB 8gj0: E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1)

All present enzymatic activity of E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1):
2.7.7.7;

The structure of E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1) also contains other interesting chemical elements:

Aluminium	(Al)	3 atoms
Fluorine	(F)	12 atoms
Zinc	(Zn)	4 atoms

The binding sites of Magnesium atom in the E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1) (pdb code 8gj0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1), PDB code: 8gj0:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg702 b:72.2 occ:1.00 F1 B:ALF703 2.1 100.1 1.0 OG1 B:THR52 2.1 78.6 1.0 O2B B:ADP701 2.1 108.1 1.0 F4 B:ALF703 2.5 95.6 1.0 HB B:THR52 2.9 86.6 1.0 O2A B:ADP701 2.9 105.3 1.0 CB B:THR52 3.0 81.4 1.0 AL B:ALF703 3.2 90.1 1.0 PB B:ADP701 3.3 81.2 1.0 H B:THR52 3.4 86.9 1.0 PA B:ADP701 3.6 105.5 1.0 O1B B:ADP701 3.6 84.9 1.0 O3A B:ADP701 3.7 75.9 1.0 HG21 B:THR52 3.8 86.5 1.0 O1A B:ADP701 3.8 105.5 1.0 OD2 B:ASP126 3.9 112.3 1.0 OE2 C:GLU144 4.0 139.1 1.0 CG2 B:THR52 4.0 84.8 1.0 N B:THR52 4.0 79.0 1.0 CA B:THR52 4.1 82.0 1.0 OE1 C:GLU144 4.2 139.5 1.0 HH22 B:ARG11 4.4 100.0 1.0 HB2 B:LYS51 4.4 90.3 1.0 F3 B:ALF703 4.5 83.9 1.0 CD C:GLU144 4.5 141.2 1.0 HG23 B:THR52 4.5 86.3 1.0 HA B:THR52 4.6 86.3 1.0 O3B B:ADP701 4.6 89.3 1.0 HH22 C:ARG169 4.6 111.5 1.0 HE2 B:LYS51 4.7 90.5 1.0 HG22 B:THR52 4.7 86.6 1.0 F2 B:ALF703 4.7 84.8 1.0 HH12 C:ARG169 4.7 110.4 1.0 CG B:ASP126 4.8 110.4 1.0 OD1 B:ASP126 4.9 114.4 1.0

Magnesium binding site 2 out of 3 in the E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg702 b:110.7 occ:1.00 OG1 C:THR52 2.1 87.3 1.0 F1 D:ALF701 2.1 112.7 1.0 O2B C:ADP701 2.1 124.2 1.0 F4 D:ALF701 2.6 123.2 1.0 HB C:THR52 3.0 91.2 1.0 CB C:THR52 3.1 80.1 1.0 AL D:ALF701 3.2 114.3 1.0 PB C:ADP701 3.3 117.1 1.0 H C:THR52 3.6 92.8 1.0 O1B C:ADP701 3.6 111.8 1.0 O1A C:ADP701 3.7 124.2 1.0 HG21 C:THR52 3.7 93.2 1.0 OD2 C:ASP126 3.7 135.4 1.0 O2A C:ADP701 3.9 110.1 1.0 HH22 D:ARG169 4.0 95.5 1.0 CG2 C:THR52 4.0 90.3 1.0 PA C:ADP701 4.0 114.6 1.0 O3A C:ADP701 4.0 103.1 1.0 N C:THR52 4.2 85.6 1.0 CA C:THR52 4.2 80.8 1.0 OE1 D:GLU144 4.4 115.5 1.0 OD1 C:ASP126 4.5 131.8 1.0 HH21 D:ARG169 4.5 96.4 1.0 F3 D:ALF701 4.5 103.0 1.0 CG C:ASP126 4.5 134.4 1.0 O3B C:ADP701 4.5 113.5 1.0 HA C:THR52 4.6 91.8 1.0 HG23 C:THR52 4.6 91.5 1.0 NH2 D:ARG169 4.6 101.5 1.0 HB2 C:LYS51 4.7 102.1 1.0 HH22 C:ARG215 4.7 103.6 1.0 OE2 D:GLU144 4.7 118.3 1.0 HG22 C:THR52 4.7 92.7 1.0 HE3 C:LYS51 4.7 102.2 1.0 HH22 C:ARG11 4.8 114.5 1.0 F2 D:ALF701 4.8 100.8 1.0 HH12 C:ARG215 4.9 105.5 1.0

Magnesium binding site 3 out of 3 in the E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of E. Coli Clamp Loader with Open Clamp on Primed Template Dna (Form 1) within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg703 b:109.8 occ:1.00 HG1 D:THR52 1.8 84.2 1.0 O2B D:ADP702 2.0 120.8 1.0 F1 D:ALF704 2.1 121.0 1.0 OG1 D:THR52 2.1 81.5 1.0 O3A D:ADP702 2.8 102.9 1.0 F3 D:ALF704 2.9 106.2 1.0 PB D:ADP702 2.9 113.1 1.0 HB D:THR52 3.1 85.0 1.0 CB D:THR52 3.2 85.9 1.0 AL D:ALF704 3.4 107.4 1.0 H D:THR52 3.5 84.4 1.0 O1B D:ADP702 3.7 94.9 1.0 HH22 D:ARG215 3.7 101.1 1.0 OD2 D:ASP126 3.7 118.0 1.0 HG21 D:THR52 3.9 82.5 1.0 OE2 E:GLU133 3.9 116.9 1.0 HH22 D:ARG11 4.1 92.1 1.0 CG2 D:THR52 4.1 78.7 1.0 N D:THR52 4.1 82.9 1.0 O3B D:ADP702 4.2 111.7 1.0 OE1 E:GLU133 4.2 115.7 1.0 PA D:ADP702 4.2 91.0 1.0 CA D:THR52 4.3 78.8 1.0 NH2 D:ARG215 4.4 102.4 1.0 O2A D:ADP702 4.4 101.5 1.0 HB2 D:LYS51 4.4 82.2 1.0 OD1 D:ASP126 4.5 120.8 1.0 CD E:GLU133 4.5 115.2 1.0 CG D:ASP126 4.5 118.1 1.0 HH21 D:ARG215 4.5 100.3 1.0 F4 D:ALF704 4.5 104.7 1.0 HG23 D:THR52 4.6 82.8 1.0 HE2 D:LYS51 4.6 85.2 1.0 HH21 D:ARG11 4.7 93.4 1.0 HA D:THR52 4.7 82.0 1.0 NH2 D:ARG11 4.7 99.8 1.0 HG22 D:THR52 4.8 83.5 1.0 OE2 D:GLU127 4.9 103.8 1.0 O5' D:ADP702 4.9 108.2 1.0 HH22 E:ARG158 5.0 96.8 1.0 F2 D:ALF704 5.0 97.5 1.0

Z.-Q.Xu, A.J.Oakley, N.E.Dixon. Structural Characterisation of the Complete Cycle of Sliding Clamp Loading in E. Coli To Be Published.