Magnesium in PDB 1aus: Activated Unliganded Spinach Rubisco

All present enzymatic activity of Activated Unliganded Spinach Rubisco:
4.1.1.39;

The structure of Activated Unliganded Spinach Rubisco, PDB code: 1aus was solved by T.C.Taylor, I.Andersson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.00 / 2.20
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	157.400, 158.700, 203.000, 90.00, 90.00, 90.00
R / Rfree (%)	21.7 / 23.9

The binding sites of Magnesium atom in the Activated Unliganded Spinach Rubisco (pdb code 1aus). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Activated Unliganded Spinach Rubisco, PDB code: 1aus:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Activated Unliganded Spinach Rubisco


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Activated Unliganded Spinach Rubisco within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg476 b:17.9 occ:1.00 O L:HOH623 2.2 23.8 1.0 O L:HOH588 2.3 20.0 1.0 OD1 L:ASP203 2.3 14.8 1.0 O2 L:FMT477 2.3 16.0 1.0 O L:HOH570 2.3 31.6 1.0 OE1 L:GLU204 2.4 13.3 1.0 C L:FMT477 3.2 15.7 1.0 O1 L:FMT477 3.3 15.0 1.0 CG L:ASP203 3.3 12.4 1.0 CD L:GLU204 3.4 10.9 1.0 OE2 L:GLU204 3.7 11.3 1.0 OD2 L:ASP203 4.0 14.3 1.0 NE2 L:HIS294 4.1 9.2 1.0 N L:GLU204 4.2 9.7 1.0 CB L:ASP203 4.3 10.4 1.0 CA L:ASP203 4.3 9.0 1.0 O L:HOH654 4.3 40.4 1.0 O L:HOH655 4.4 28.6 1.0 NZ L:LYS177 4.4 15.5 1.0 NZ L:LYS201 4.4 13.5 1.0 CG2 L:THR173 4.6 14.5 1.0 CG L:GLU204 4.8 11.1 1.0 C L:ASP203 4.8 9.9 1.0 CE L:LYS177 4.8 15.2 1.0 CE1 L:HIS294 4.9 9.0 1.0 CD2 L:HIS294 4.9 9.2 1.0

Magnesium binding site 2 out of 4 in the Activated Unliganded Spinach Rubisco


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Activated Unliganded Spinach Rubisco within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg476 b:17.9 occ:1.00 OD1 M:ASP203 2.3 14.8 1.0 O2 M:FMT477 2.3 16.0 1.0 OE1 M:GLU204 2.4 13.3 1.0 C M:FMT477 3.2 15.7 1.0 O1 M:FMT477 3.3 15.0 1.0 CG M:ASP203 3.3 12.4 1.0 CD M:GLU204 3.4 10.9 1.0 OE2 M:GLU204 3.7 11.3 1.0 OD2 M:ASP203 4.0 14.3 1.0 NE2 M:HIS294 4.1 9.2 1.0 N M:GLU204 4.2 9.7 1.0 CB M:ASP203 4.3 10.4 1.0 CA M:ASP203 4.3 9.0 1.0 NZ M:LYS177 4.4 15.5 1.0 NZ M:LYS201 4.4 13.5 1.0 CG2 M:THR173 4.6 14.5 1.0 CG M:GLU204 4.8 11.1 1.0 C M:ASP203 4.8 9.9 1.0 CE M:LYS177 4.8 15.2 1.0 CE1 M:HIS294 4.9 9.0 1.0 CD2 M:HIS294 4.9 9.2 1.0

Magnesium binding site 3 out of 4 in the Activated Unliganded Spinach Rubisco


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Activated Unliganded Spinach Rubisco within 5.0Å range: probe atom residue distance (Å) B Occ N:Mg476 b:17.9 occ:1.00 OD1 N:ASP203 2.3 14.8 1.0 O2 N:FMT477 2.3 16.0 1.0 OE1 N:GLU204 2.4 13.3 1.0 C N:FMT477 3.2 15.7 1.0 O1 N:FMT477 3.3 15.0 1.0 CG N:ASP203 3.3 12.4 1.0 CD N:GLU204 3.4 10.9 1.0 OE2 N:GLU204 3.7 11.3 1.0 OD2 N:ASP203 4.0 14.3 1.0 NE2 N:HIS294 4.1 9.2 1.0 N N:GLU204 4.2 9.7 1.0 CB N:ASP203 4.3 10.4 1.0 CA N:ASP203 4.3 9.0 1.0 NZ N:LYS177 4.4 15.5 1.0 NZ N:LYS201 4.4 13.5 1.0 CG2 N:THR173 4.6 14.5 1.0 CG N:GLU204 4.8 11.1 1.0 C N:ASP203 4.8 9.9 1.0 CE N:LYS177 4.8 15.2 1.0 CE1 N:HIS294 4.9 9.0 1.0 CD2 N:HIS294 4.9 9.2 1.0

Magnesium binding site 4 out of 4 in the Activated Unliganded Spinach Rubisco


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Activated Unliganded Spinach Rubisco within 5.0Å range: probe atom residue distance (Å) B Occ O:Mg476 b:17.9 occ:1.00 OD1 O:ASP203 2.3 14.8 1.0 O2 O:FMT477 2.3 16.0 1.0 OE1 O:GLU204 2.4 13.3 1.0 C O:FMT477 3.2 15.7 1.0 O1 O:FMT477 3.3 15.0 1.0 CG O:ASP203 3.3 12.4 1.0 CD O:GLU204 3.4 10.9 1.0 OE2 O:GLU204 3.7 11.3 1.0 OD2 O:ASP203 4.0 14.3 1.0 NE2 O:HIS294 4.1 9.2 1.0 N O:GLU204 4.2 9.7 1.0 CB O:ASP203 4.3 10.4 1.0 CA O:ASP203 4.3 9.0 1.0 NZ O:LYS177 4.4 15.5 1.0 NZ O:LYS201 4.4 13.5 1.0 CG2 O:THR173 4.6 14.5 1.0 CG O:GLU204 4.8 11.1 1.0 C O:ASP203 4.8 9.9 1.0 CE O:LYS177 4.8 15.2 1.0 CE1 O:HIS294 4.9 9.0 1.0 CD2 O:HIS294 4.9 9.2 1.0

T.C.Taylor, I.Andersson. Structure of A Product Complex of Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Biochemistry V. 36 4041 1997.
ISSN: ISSN 0006-2960
PubMed: 9092835
DOI: 10.1021/BI962818W