Magnesium in PDB 1azt: Gs-Alpha Complexed with Gtp-Gamma-S

The structure of Gs-Alpha Complexed with Gtp-Gamma-S, PDB code: 1azt was solved by J.J.G.Tesmer, S.R.Sprang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	88.300, 96.500, 133.400, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 28.2

The binding sites of Magnesium atom in the Gs-Alpha Complexed with Gtp-Gamma-S (pdb code 1azt). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Gs-Alpha Complexed with Gtp-Gamma-S, PDB code: 1azt:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Gs-Alpha Complexed with Gtp-Gamma-S


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Gs-Alpha Complexed with Gtp-Gamma-S within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:14.5 occ:1.00 O A:HOH415 1.9 10.9 1.0 OG A:SER54 2.0 28.2 1.0 O2G A:GSP413 2.4 36.5 1.0 OG1 A:THR204 2.4 25.6 1.0 O2B A:GSP413 2.5 18.5 1.0 CB A:THR204 3.1 25.1 1.0 OD2 A:ASP223 3.3 29.8 1.0 CB A:SER54 3.3 19.9 1.0 OD1 A:ASP223 3.6 30.3 1.0 PG A:GSP413 3.6 31.0 1.0 PB A:GSP413 3.6 20.8 1.0 O3B A:GSP413 3.8 27.3 1.0 N A:SER54 3.8 14.6 1.0 CG A:ASP223 3.8 31.4 1.0 CG2 A:THR204 3.9 22.8 1.0 O A:VAL224 3.9 39.6 1.0 CA A:SER54 4.1 12.4 1.0 CA A:THR204 4.3 28.8 1.0 O3G A:GSP413 4.3 30.1 1.0 CB A:LYS53 4.4 11.1 1.0 N A:THR204 4.4 28.3 1.0 O1B A:GSP413 4.6 21.1 1.0 CE A:LYS53 4.6 7.2 1.0 O A:HOH435 4.6 37.5 1.0 C A:LYS53 4.8 16.8 1.0 O3A A:GSP413 4.8 19.5 1.0 O1 A:PO4406 4.8 85.1 1.0 O1A A:GSP413 4.8 26.5 1.0 O A:VAL202 4.8 30.2 1.0 O A:HOH438 4.9 31.8 1.0

Magnesium binding site 2 out of 2 in the Gs-Alpha Complexed with Gtp-Gamma-S


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Gs-Alpha Complexed with Gtp-Gamma-S within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg406 b:47.5 occ:1.00 OG B:SER54 1.9 21.6 1.0 OG1 B:THR204 1.9 21.4 1.0 O2G B:GSP411 2.6 28.3 1.0 O2B B:GSP411 2.7 13.6 1.0 CB B:THR204 2.9 24.2 1.0 O B:HOH413 3.0 13.2 1.0 OD2 B:ASP223 3.0 27.4 1.0 CB B:SER54 3.2 12.8 1.0 CG2 B:THR204 3.3 19.6 1.0 OD1 B:ASP223 3.4 18.6 1.0 CG B:ASP223 3.6 25.7 1.0 O B:VAL224 3.7 28.9 1.0 N B:SER54 3.8 4.8 1.0 PG B:GSP411 3.9 29.2 1.0 CA B:SER54 3.9 9.8 1.0 PB B:GSP411 4.1 6.6 1.0 CA B:THR204 4.2 26.9 1.0 O3B B:GSP411 4.4 16.0 1.0 O3G B:GSP411 4.5 27.6 1.0 N B:THR204 4.6 26.3 1.0 O2A B:GSP411 4.7 16.0 1.0 CB B:LYS53 4.7 2.0 1.0 C B:VAL224 4.8 27.8 1.0 O1B B:GSP411 4.8 10.8 1.0 C B:LYS53 4.9 3.3 1.0 O1 B:PO4408 4.9 59.6 1.0

R.K.Sunahara, J.J.Tesmer, A.G.Gilman, S.R.Sprang. Crystal Structure of the Adenylyl Cyclase Activator Gsalpha Science V. 278 1943 1997.
ISSN: ISSN 0036-8075
PubMed: 9395396
DOI: 10.1126/SCIENCE.278.5345.1943